Fitness for 5 genes in Escherichia coli BW25113

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500 ntccmH and ccmG overlap by 4 nucleotidesccmG and ccmF overlap by 4 nucleotidesccmF and ccmE overlap by 4 nucleotidesccmE and ccmD overlap by 4 nucleotides b2194: ccmH - heme lyase, CcmH subunit (NCBI), at 2,289,380 to 2,290,432 ccmH b2195: ccmG - periplasmic thioredoxin of cytochrome c-type biogenesis (NCBI), at 2,290,429 to 2,290,986 ccmG b2196: ccmF - heme lyase, CcmF subunit (NCBI), at 2,290,983 to 2,292,926 ccmF b2197: ccmE - periplasmic heme chaperone (NCBI), at 2,292,923 to 2,293,402 ccmE b2198: ccmD - cytochrome c biogenesis protein (NCBI), at 2,293,399 to 2,293,608 ccmD
Group Condition b2194 b2195 b2196 b2197 b2198
ccmH ccmG ccmF ccmE ccmD
phage T7_phage 0.0015625 MOI TC2 +0.2 -6.0 +0.2 -0.2 +0.1
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.3 -0.5 -0.8 -0.7 -0.5
gfp_control GFP_control -0.2 -0.4 -0.2 -0.2 -1.2
stress Cisplatin 0.1 mg/ml -0.1 -1.5 +0.4 -0.4 -0.7
msc 22hr_MSC_rep1_highinoculum -0.3 -0.1 -0.2 +0.3 -1.4
stress Tetracycline 0.0008 mg/ml -0.4 -0.8 -0.1 -0.6 +0.3
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.4 -0.7 -0.0 -0.8 +0.4
mzaabcde mzaABCDE -0.1 +0.4 -0.3 -0.1 -1.5
carbon source carbon source D-Mannose 0.5mM -0.3 -0.5 +0.4 -0.7 -0.5
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.4 -0.1 +0.4 +0.4 -1.4
phage R2_N4_T1_phage10 +0.0 +0.7 -0.7 -1.0 +0.0
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.2 +0.9 -0.2 +0.3 -1.7
control PBS_cage_1_mouse_1_Cecum_40 hr -0.4 -0.9 -0.4 -0.0 +0.8
phage T7_phage 0.00015625 MOI TC3 +0.2 -0.8 +0.3 -0.8 +0.3
stress LB Anaerobic with Ampicillin 0.00000195313 mM +0.2 +0.5 -0.4 +0.4 -0.8
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.3 -1.4 +0.3 +0.0 +0.6
phage Ste41 -0.5 -1.1 +0.5 +0.6 +0.4
stress Cephalothin 0.01 mg/ml +0.2 -1.2 +0.2 -0.1 +0.8
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.2 +0.5 +0.5 -0.9 +0.3
stress Benzalkonium Chloride 0.004 mg/ml -0.0 -1.3 +0.3 +0.5 +0.8
phage RB68 -0.1 -0.6 +0.5 +0.3 +0.7
stress LB Aerobic with Ramoplanin 0.000125 mM +0.3 +1.1 -0.3 +0.2 -0.4
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.2 -0.5 +0.1 +1.1 +0.7
stress Ciprofloxacin Hydrochloride 0.0003 mM +0.4 +0.9 -0.2 -0.5 +0.9
stress 1-ethyl-3-methylimidazolium acetate 60 mM +0.1 +0.8 -0.2 -0.2 +1.0
control PBS_cage_1_mice _Feces_22 hr -0.4 +0.8 -0.1 -0.2 +1.6
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.2 +0.2 +0.0 +0.8 +1.1
control PBS_cage_1_mice _Feces_40 hr -0.3 +0.8 -0.2 -0.1 +1.7
phage N4_phage 0.009375 MOI TC2 +0.1 +1.1 +0.2 +0.1 +1.0
control PBS_cage_1_mice _Feces_16 hr -0.2 +1.2 +0.2 -0.5 +2.2
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