Fitness for 5 genes in Escherichia coli BW25113

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500 ntyeiM and yeiN are separated by 93 nucleotidesyeiN and yeiC overlap by 13 nucleotidesyeiC and fruA are separated by 422 nucleotidesfruA and fruK are separated by 16 nucleotides b2164: yeiM - predicted nucleoside transporter (NCBI), at 2,254,107 to 2,255,357 yeiM b2165: yeiN - hypothetical protein (NCBI), at 2,255,451 to 2,256,389 yeiN b2166: yeiC - predicted kinase (NCBI), at 2,256,377 to 2,257,318 yeiC b2167: fruA - fused fructose-specific PTS enzymes: IIBcomponent/IIC components (NCBI), at 2,257,741 to 2,259,432 fruA b2168: fruK - 1-phosphofructokinase (NCBI), at 2,259,449 to 2,260,387 fruK
Group Condition b2164 b2165 b2166 b2167 b2168
yeiM yeiN yeiC fruA fruK
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr +0.2 -0.8 +0.4 -0.3 -6.6
control PBS_cage_1_mouse_1_Cecum_40 hr +0.1 -1.1 +0.0 +0.2 -6.3
control PBS_cage_1_mouse_2_Cecum_40 hr +0.1 +0.6 +0.2 -0.4 -6.7
carbon source carbon source D-Mannose 1mM +0.0 -1.6 +0.0 -0.5 -4.0
msc 22hr_MSC_rep1_highinoculum -0.0 -0.4 -0.3 -0.2 -5.0
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.4 -0.3 -0.4 -0.2 -4.4
carbon source Ying_OrganicAcid7 rep B; time point 3 -0.1 +0.4 -0.2 -0.1 -5.3
carbon source D-Serine (C) -0.0 +0.0 +0.0 +0.1 -5.4
carbon source Acetate (C) -0.4 +0.3 -0.2 +0.1 -5.0
carbon source Ying_OrganicAcid7 rep A; time point 3 -0.2 +0.6 -0.1 -0.1 -5.1
phage T7_phage 0.0015625 MOI TC2 +0.2 -0.4 -0.3 -0.4 -3.9
carbon source carbon source D-Mannose 0.5mM +0.4 -0.6 -0.6 -0.1 -3.7
msc 22hr_mediaonly_rep1_lowinoculum -0.1 +0.4 +0.0 -0.1 -4.8
carbon source pyruvate (C) -0.1 -0.0 -0.0 -0.0 -4.5
phage Δdbn_10 +0.2 -0.9 -0.1 -0.4 -3.3
phage T7_phage 0.0000015625 MOI TC3 +0.1 +0.1 -0.4 -0.2 -4.1
carbon source carbon source D-Mannose 1mM -0.2 -0.0 -0.2 -0.4 -3.7
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=None +0.0 -0.3 -0.2 -0.1 -3.5
carbon source carbon source D-Glucose 0.25mM +0.5 -0.9 -0.1 +0.1 -3.7
stress LB Aerobic with Ramoplanin 0.000125 mM -0.1 -0.7 -0.8 +0.1 -2.4
carbon source D-Fructose (C) +0.1 +0.0 +0.0 -1.3 -2.9
carbon source D,L-Lactate (C) +0.3 -0.1 -0.0 +0.0 -4.1
motility outer cut, LB soft agar motility assay +0.4 -0.5 -0.4 -0.2 -3.2
carbon source Ying_Nucleobase14 rep C; time point 2 -0.1 +0.1 -0.1 -0.1 -3.7
carbon source D-Fructose (C) +0.1 +0.1 -0.0 -1.6 -2.2
carbon source Ying_Others16 rep B; time point 2 +0.0 +0.5 +0.2 +0.0 -4.4
stress Chlorite 0.4 mM +0.2 -0.1 -0.2 +0.1 -3.4
carbon source D-Serine (C) -0.1 +0.4 -0.1 +0.1 -3.6
control PBS_cage_2_mice _Feces_16 hr -0.1 +0.5 +0.1 -0.1 -3.6
stress Chlorite 1.25 mM +0.6 -0.2 -0.2 +1.6 -2.6
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