Fitness for 5 genes in Escherichia coli BW25113

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500 ntmglA and mglB are separated by 60 nucleotidesmglB and galS are separated by 279 nucleotidesgalS and yeiB are separated by 141 nucleotidesyeiB and folE are separated by 16 nucleotides b2149: mglA - fused methyl-galactoside transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 2,235,791 to 2,237,311 mglA b2150: mglB - methyl-galactoside transporter subunit (NCBI), at 2,237,372 to 2,238,370 mglB b2151: galS - DNA-binding transcriptional repressor (NCBI), at 2,238,650 to 2,239,690 galS b2152: yeiB - conserved inner membrane protein (NCBI), at 2,239,832 to 2,240,989 yeiB b2153: folE - GTP cyclohydrolase I (NCBI), at 2,241,006 to 2,241,674 folE
Group Condition b2149 b2150 b2151 b2152 b2153
mglA mglB galS yeiB folE
carbon source succinate (C) +0.4 -0.5 -1.8 -0.5 N.D.
carbon source L-Malic (C) +0.4 -0.1 -2.0 -0.4 N.D.
carbon source carbon source D-Mannose 5mM -1.0 -0.4 +0.5 -1.2 N.D.
carbon source carbon source D-Mannose 1mM -1.2 -0.3 +0.3 -0.7 N.D.
carbon source D-Galacturonic Acid (C) M9 -0.6 -1.0 +0.2 -0.4 N.D.
carbon source succinate (C) +0.3 -0.4 -1.4 -0.3 N.D.
carbon source L-Malic (C) +0.4 -0.0 -1.7 -0.2 N.D.
stress Ciprofloxacin Hydrochloride 0.0024 mM -0.5 -0.6 +0.2 -0.6 N.D.
carbon source D-Glucosamine Hydrochloride (C) -0.2 -1.0 +0.3 -0.5 N.D.
carbon source D-Galacturonic Acid (C) MOPS -0.3 -0.8 +0.2 -0.5 N.D.
stress Ciprofloxacin Hydrochloride 0.0003 mM +0.1 +0.0 -0.8 -0.7 N.D.
carbon source Glycolic Acid (C) -0.3 -0.5 +0.2 -0.7 N.D.
carbon source acetate (C) -0.2 -0.8 +0.5 -0.7 N.D.
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.1 -1.1 -0.3 +0.3 N.D.
stress 2-nitrobenzenesulfonyl fluoride +0.2 -0.6 -0.7 +0.2 N.D.
phage RB68 -0.5 -1.1 +0.2 +0.5 N.D.
carbon source D-Lactate (C) -0.1 -0.5 +0.4 -0.5 N.D.
phage Ste41 -0.5 -0.7 +0.2 +0.3 N.D.
phage Ste41 -0.2 -0.9 -0.3 +0.7 N.D.
carbon source D-Trehalose (C) -0.3 -0.7 +0.7 -0.4 N.D.
carbon source D-Trehalose (C) -0.3 -0.8 +0.9 -0.2 N.D.
stress Benzalkonium Chloride 0.004 mg/ml -0.4 -0.7 +0.7 +0.2 N.D.
carbon source carbon source D-Mannose 0.5mM -0.8 +0.2 +0.4 +0.1 N.D.
carbon source D-Galactose (C) -0.8 -1.4 +2.3 -0.1 N.D.
carbon source D-Galactose (C) -0.6 -0.9 +2.2 -0.1 N.D.
phage T7_phage 0.00015625 MOI TC4 +0.3 +0.8 -0.0 +0.6 N.D.
predation 6 hr rep2_HD100_10fold_dilution_grewON +0.3 +0.6 +0.1 +0.7 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.5 +1.8 -0.1 -0.2 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.6 +2.3 +0.2 +0.0 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.7 +2.3 +0.1 +0.4 N.D.
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