Fitness for 5 genes in Escherichia coli BW25113

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500 ntdusC and yohJ are separated by 238 nucleotidesyohJ and yohK overlap by 4 nucleotidesyohK and cdd are separated by 129 nucleotidescdd and sanA are separated by 149 nucleotides b2140: dusC - tRNA-dihydrouridine synthase C (NCBI), at 2,227,460 to 2,228,407 dusC b2141: yohJ - hypothetical protein (NCBI), at 2,228,646 to 2,229,044 yohJ b2142: yohK - predicted inner membrane protein (NCBI), at 2,229,041 to 2,229,736 yohK b2143: cdd - cytidine deaminase (NCBI), at 2,229,866 to 2,230,750 cdd b2144: sanA - hypothetical protein (NCBI), at 2,230,900 to 2,231,619 sanA
Group Condition b2140 b2141 b2142 b2143 b2144
dusC yohJ yohK cdd sanA
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.3 -0.3 -4.3 -0.5 -2.8
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.3 -4.6 -2.9 -0.0 -0.3
stress Spectinomycin 0.0125 mg/ml +0.0 -0.9 -0.2 -4.1 -0.1
stress M9 with Methotrexate 1.98 mM 48 hr -0.1 -0.6 -0.1 -0.1 -4.1
stress 1-ethyl-3-methylimidazolium chloride 40 mM +0.2 -0.1 -0.4 -4.6 +0.2
stress M9 with Methotrexate 1.98 mM 48 hr -0.1 -0.3 -0.0 -0.2 -3.8
stress LB Anaerobic with Spiramycin 0.000125 mM +0.1 -0.6 -1.0 -0.7 -1.7
stress M9 with Methotrexate 1.98 mM 24 hr +0.0 -0.6 -0.1 -0.0 -3.0
stress M9 with Methotrexate 1.98 mM 24 hr +0.1 -0.4 -0.1 +0.2 -3.3
stress 1-ethyl-3-methylimidazolium acetate 30 mM +0.0 -0.5 -0.1 -3.1 +0.2
stress 1-ethyl-3-methylimidazolium chloride 80 mM +0.0 +0.3 -0.4 -3.7 +0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.1 -3.4 -0.3 +0.4 -0.1
stress M9 with Methotrexate 1.98 mM 24 hr +0.1 -0.3 -0.0 +0.2 -3.2
stress LB Aerobic with Ramoplanin 0.000125 mM -0.2 -0.1 +0.4 -0.8 -2.2
stress Benzalkonium Chloride 0.004 mg/ml +0.5 -0.4 -1.5 -1.4 +0.1
carbon source Cytidine 0.41115 mM (C) +0.2 +0.3 +0.0 -2.6 -0.6
carbon source acetate (C) +0.1 +0.9 +0.5 -0.7 -3.3
carbon source acetate (C) +0.0 +0.2 +0.4 -0.6 -2.6
stress Trimethoprim 0.00125 mM -0.1 -0.0 +0.3 +0.4 -2.8
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.6 -1.0 -1.9 +0.4 -0.1
carbon source D-Ribose (C) +0.1 +0.4 +0.2 -0.7 -2.1
stress Trimethoprim 0.0025 mM -0.2 -0.5 +0.2 +0.9 -2.4
carbon source a-Ketoglutaric (C) -0.0 +0.7 +0.4 -0.3 -2.5
carbon source Acetate (C) +0.1 +0.8 +0.4 -0.4 -1.8
carbon source Glycolic Acid (C) +0.3 +1.9 +1.3 -0.3 -3.1
carbon source Glycolic Acid (C) +0.2 +1.3 +1.6 -0.4 -2.4
phage Papa dilution 10-1 cacl2 maltose +0.5 +0.4 +1.1 +0.9 +0.8
carbon source carbon source D-Glucose 20mM +0.1 +0.0 -0.1 +0.3 +3.5
carbon source carbon source D-Glucose 20mM +0.1 +0.4 +0.0 -0.3 +3.7
carbon source carbon source D-Glucose 5mM +0.1 +0.7 +0.7 +0.2 +3.0
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