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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yohP and dusC are separated by 372 nucleotides
dusC and yohJ are separated by 238 nucleotides
yohJ and yohK overlap by 4 nucleotides
yohK and cdd are separated by 129 nucleotides
b4679: yohP - expressed protein (RefSeq), at 2,227,004 to 2,227,087
yohP
b2140: dusC - tRNA-dihydrouridine synthase C (NCBI), at 2,227,460 to 2,228,407
dusC
b2141: yohJ - hypothetical protein (NCBI), at 2,228,646 to 2,229,044
yohJ
b2142: yohK - predicted inner membrane protein (NCBI), at 2,229,041 to 2,229,736
yohK
b2143: cdd - cytidine deaminase (NCBI), at 2,229,866 to 2,230,750
cdd
Group
Condition
b4679
b2140
b2141
b2142
b2143
yohP
dusC
yohJ
yohK
cdd
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
+0.4
+0.3
-4.6
-2.9
-0.0
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.9
+0.3
-0.3
-4.3
-0.5
stress
Spectinomycin 0.0125 mg/ml
-0.4
+0.0
-0.9
-0.2
-4.1
stress
1-ethyl-3-methylimidazolium chloride 40 mM
-0.1
+0.2
-0.1
-0.4
-4.6
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-3.5
-0.6
-0.9
-0.2
+0.7
stress
1-ethyl-3-methylimidazolium chloride 80 mM
-0.6
+0.0
+0.3
-0.4
-3.7
stress
1-ethyl-3-methylimidazolium acetate 30 mM
-0.5
+0.0
-0.5
-0.1
-3.1
stress
Benzalkonium Chloride 0.004 mg/ml
-1.0
+0.5
-0.4
-1.5
-1.4
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.6
-0.1
-1.3
-0.4
-0.9
stress
1-ethyl-3-methylimidazolium acetate 60 mM
-0.2
+0.0
-0.3
-0.2
-2.5
balo
Mixed culture, Halobacteriovorax-10
-1.2
-0.1
-0.9
+0.2
-1.0
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-2.8
-0.4
+0.1
-0.1
+0.6
predation
6 hr rep1_HD100_10fold_dilution_grewON
-2.6
+0.3
+0.5
-0.7
+0.0
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+1.1
-0.1
-3.4
-0.3
+0.4
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.1
+0.6
-1.0
-1.9
+0.4
carbon source
Cytidine 0.41115 mM (C)
+0.1
+0.2
+0.3
+0.0
-2.6
carbon source
Cytidine 0.41115 mM (C)
+0.6
-0.1
-0.0
-0.2
-2.1
phage
T7_phage 0.00015625 MOI TC4
-1.6
-0.6
-0.2
+0.3
+0.5
stress
Aluminum chloride 5 mM
-0.5
+0.1
-1.9
+0.8
+0.1
motility
outer cut, LB soft agar motility assay
+0.9
-0.7
-0.6
-0.2
-0.7
carbon source
carbon source D-Mannose 1mM
-2.2
+0.1
-0.0
+0.5
+0.4
carbon source
carbon source D-Mannose 0.5mM
-1.5
-0.0
+0.8
+0.3
-0.5
carbon source
Cytidine 0.41115 mM (C)
+0.6
-0.0
+0.4
+0.1
-1.8
carbon source
carbon source D-Glucose 5mM
-2.2
+0.1
+0.7
+0.7
+0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.4
+0.4
-0.5
+0.7
-1.0
carbon source
acetate (C)
+0.9
+0.1
+0.9
+0.5
-0.7
carbon source
carbon source D-Glucose 0.25mM
-0.2
+0.2
+1.5
-0.0
+1.1
carbon source
Glycolic Acid (C)
+0.6
+0.2
+1.3
+1.6
-0.4
phage
Papa dilution 10-1 cacl2 maltose
+0.7
+0.5
+0.4
+1.1
+0.9
carbon source
Glycolic Acid (C)
+0.9
+0.3
+1.9
+1.3
-0.3
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