Fitness for 5 genes in Escherichia coli BW25113

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500 ntyehZ and bglX are separated by 210 nucleotidesbglX and dld are separated by 195 nucleotidesdld and pbpG are separated by 37 nucleotidespbpG and yohC are separated by 173 nucleotides b2131: yehZ - predicted transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 2,216,586 to 2,217,503 yehZ b2132: bglX - beta-D-glucoside glucohydrolase, periplasmic (NCBI), at 2,217,714 to 2,220,011 bglX b2133: dld - D-lactate dehydrogenase, FAD-binding, NADH independent (NCBI), at 2,220,207 to 2,221,922 dld b2134: pbpG - penicillin-binding protein 7 (VIMSS), at 2,221,960 to 2,222,892 pbpG b2135: yohC - orf, hypothetical protein (VIMSS), at 2,223,066 to 2,223,653 yohC
Group Condition b2131 b2132 b2133 b2134 b2135
yehZ bglX dld pbpG yohC
stress Aluminum chloride 5 mM -0.2 -0.8 -0.6 -1.2 +0.5
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.3 -0.9 -0.2 -0.5 -0.3
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.4 -1.5 -0.1 +0.2 -0.2
phage RB68 +0.2 +0.1 -0.1 -1.0 -1.1
motility inner cut, LB soft agar motility assay -0.6 -0.1 +0.1 -0.5 -0.6
carbon source carbon source D-Glucose 0.25mM -1.0 +0.1 -0.2 -0.3 -0.3
stress Cisplatin 0.1 mg/ml -0.9 -0.4 -0.3 +0.2 -0.2
carbon source D-Lactate (C) -0.3 -0.1 -1.4 +0.2 +0.1
stress LB Aerobic with Ramoplanin 0.000125 mM +0.3 +0.8 -0.3 -2.2 -0.0
phage Ste41 +0.6 +0.1 -0.4 -1.4 -0.3
carbon source D-Lactate (C) -0.1 -0.1 -1.3 +0.3 -0.1
phage RB68 +0.4 -0.1 -0.2 -0.3 -0.9
stress LB Aerobic with Ramoplanin 0.000125 mM +0.1 +1.1 -0.3 -1.7 -0.3
stress Benzalkonium Chloride 0.004 mg/ml -1.7 -1.1 +0.1 +1.5 +0.3
carbon source carbon source D-Mannose 5mM -0.5 +0.2 -0.1 +0.5 -0.8
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.1 +0.3 +0.3 -0.7 -0.5
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.0 -0.1 -1.1 +0.7 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.4 -0.2 -1.0 -0.2 +0.4
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.0 -0.2 -1.2 +0.8 +0.1
stress 1-ethyl-3-methylimidazolium chloride 80 mM +0.1 -1.1 +0.2 +0.7 -0.1
phage T7_phage 0.00015625 MOI TC4 +0.6 +0.5 -0.1 -1.1 -0.2
stress LB Aerobic with Irgasan 0.0000003125 mM -0.7 +0.1 -0.3 +0.0 +0.8
stress Chlorite 1.25 mM -0.6 -0.1 +0.3 +0.7 -0.5
stress Trimethoprim 0.005 mM +0.8 -0.3 +0.5 -0.6 +0.2
stress Doxycycline hyclate 0.001 mg/ml -0.3 +0.4 -0.1 +0.9 -0.3
stress octanol 0.08 vol% -0.5 +0.2 +0.2 +1.1 -0.0
retron_ec_48_control retron_Ec_48_control +0.3 +0.3 +0.4 +0.8 -0.2
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.1 +1.1 +0.2 +1.7 -0.3
stress Bacitracin 1 mg/ml +0.2 +0.7 -0.0 +1.3 +0.4
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.5 +1.3 +0.1 +1.6 -0.2
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