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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
yehZ and bglX are separated by 210 nucleotides
bglX and dld are separated by 195 nucleotides
dld and pbpG are separated by 37 nucleotides
pbpG and yohC are separated by 173 nucleotides
b2131: yehZ - predicted transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 2,216,586 to 2,217,503
yehZ
b2132: bglX - beta-D-glucoside glucohydrolase, periplasmic (NCBI), at 2,217,714 to 2,220,011
bglX
b2133: dld - D-lactate dehydrogenase, FAD-binding, NADH independent (NCBI), at 2,220,207 to 2,221,922
dld
b2134: pbpG - penicillin-binding protein 7 (VIMSS), at 2,221,960 to 2,222,892
pbpG
b2135: yohC - orf, hypothetical protein (VIMSS), at 2,223,066 to 2,223,653
yohC
Group
Condition
b2131
b2132
b2133
b2134
b2135
yehZ
bglX
dld
pbpG
yohC
stress
Aluminum chloride 5 mM
-0.2
-0.8
-0.6
-1.2
+0.5
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.3
-0.9
-0.2
-0.5
-0.3
stress
LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.4
-1.5
-0.1
+0.2
-0.2
phage
RB68
+0.2
+0.1
-0.1
-1.0
-1.1
motility
inner cut, LB soft agar motility assay
-0.6
-0.1
+0.1
-0.5
-0.6
carbon source
carbon source D-Glucose 0.25mM
-1.0
+0.1
-0.2
-0.3
-0.3
stress
Cisplatin 0.1 mg/ml
-0.9
-0.4
-0.3
+0.2
-0.2
carbon source
D-Lactate (C)
-0.3
-0.1
-1.4
+0.2
+0.1
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.3
+0.8
-0.3
-2.2
-0.0
phage
Ste41
+0.6
+0.1
-0.4
-1.4
-0.3
carbon source
D-Lactate (C)
-0.1
-0.1
-1.3
+0.3
-0.1
phage
RB68
+0.4
-0.1
-0.2
-0.3
-0.9
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.1
+1.1
-0.3
-1.7
-0.3
stress
Benzalkonium Chloride 0.004 mg/ml
-1.7
-1.1
+0.1
+1.5
+0.3
carbon source
carbon source D-Mannose 5mM
-0.5
+0.2
-0.1
+0.5
-0.8
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-0.1
+0.3
+0.3
-0.7
-0.5
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.0
-0.1
-1.1
+0.7
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.4
-0.2
-1.0
-0.2
+0.4
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.0
-0.2
-1.2
+0.8
+0.1
stress
1-ethyl-3-methylimidazolium chloride 80 mM
+0.1
-1.1
+0.2
+0.7
-0.1
phage
T7_phage 0.00015625 MOI TC4
+0.6
+0.5
-0.1
-1.1
-0.2
stress
LB Aerobic with Irgasan 0.0000003125 mM
-0.7
+0.1
-0.3
+0.0
+0.8
stress
Chlorite 1.25 mM
-0.6
-0.1
+0.3
+0.7
-0.5
stress
Trimethoprim 0.005 mM
+0.8
-0.3
+0.5
-0.6
+0.2
stress
Doxycycline hyclate 0.001 mg/ml
-0.3
+0.4
-0.1
+0.9
-0.3
stress
octanol 0.08 vol%
-0.5
+0.2
+0.2
+1.1
-0.0
retron_ec_48_control
retron_Ec_48_control
+0.3
+0.3
+0.4
+0.8
-0.2
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.1
+1.1
+0.2
+1.7
-0.3
stress
Bacitracin 1 mg/ml
+0.2
+0.7
-0.0
+1.3
+0.4
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.5
+1.3
+0.1
+1.6
-0.2
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