Fitness for 5 genes in Escherichia coli BW25113

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500 ntyegU and yegV overlap by 4 nucleotidesyegV and yegW overlap by 27 nucleotidesyegW and yegX are separated by 51 nucleotidesyegX and thiD are separated by 64 nucleotides b2099: yegU - predicted hydrolase (NCBI), at 2,178,117 to 2,179,121 yegU b2100: yegV - predicted kinase (NCBI), at 2,179,118 to 2,180,083 yegV b2101: yegW - predicted DNA-binding transcriptional regulator (NCBI), at 2,180,057 to 2,180,803 yegW b2102: yegX - orf, hypothetical protein (VIMSS), at 2,180,855 to 2,181,673 yegX b2103: thiD - phosphomethylpyrimidine kinase (NCBI), at 2,181,738 to 2,182,538 thiD
Group Condition b2099 b2100 b2101 b2102 b2103
yegU yegV yegW yegX thiD
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.3 -0.9 -5.1 +0.2 +1.1
carbon source D-Galacturonic Acid (C) M9 -0.1 +0.1 -0.1 -0.2 -3.9
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.5 +0.4 -4.8 +0.2 +0.5
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.8 -0.5 -3.7 -0.3 -0.2
carbon source Glycolic Acid (C) -0.0 -0.2 -0.1 +0.1 -3.5
carbon source D-Serine (C) +0.1 -0.5 -0.1 -0.1 -3.1
phage T7_phage 0.0015625 MOI TC2 -0.4 -0.9 -0.9 -0.5 -0.9
nitrogen source D-Alanine (N) -0.2 -0.1 +0.1 -0.1 -3.2
carbon source D-Glucose-6-Phosphate (C) +0.1 -0.2 +0.0 -0.2 -2.9
carbon source D-Galacturonic Acid (C) MOPS +0.0 +0.3 +0.3 -0.0 -3.7
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.3 +0.1 -3.0 +0.2 -0.0
carbon source Glycolic Acid (C) +0.1 +0.1 +0.1 +0.0 -3.4
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.5 -0.4 -2.9 +0.3 +0.5
carbon source D-Glucuronic Acid (C) +0.3 -0.1 +0.1 -0.2 -2.9
protonated/deuterated protonated glucose in D2O +0.1 +0.0 +0.1 +0.2 -3.0
carbon source a-Ketoglutaric (C) -0.1 -0.1 +0.4 -0.0 -2.7
carbon source a-Ketoglutaric (C) +0.1 -0.1 +0.5 -0.3 -2.7
protonated/deuterated deuterated glucose in D2O +0.3 -0.1 +0.3 +0.2 -3.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 -0.1 -3.3 +0.4 +0.4
protonated/deuterated deuterated glucose in D2O +0.0 +0.1 +0.3 +0.2 -2.6
carbon source acetate (C) +0.2 -0.6 +1.3 -0.1 -2.5
carbon source acetate (C) +0.1 -0.3 +1.2 -0.1 -2.1
carbon source Inosine 0.3728 mM (C) -0.3 -0.1 +3.9 -0.1 -0.3
carbon source Ying_Nucleobase14 rep B; time point 2 +0.2 +0.1 +3.0 +0.0 -0.1
carbon source Ying_Nucleobase14 rep A; time point 2 +0.4 +0.0 +3.1 +0.2 -0.0
carbon source Ying_Nucleobase14 rep A; time point 3 +0.2 -0.1 +3.9 +0.0 -0.1
carbon source Ying_Nucleobase14 rep C; time point 3 +0.1 -0.1 +3.7 +0.1 +0.1
carbon source Ying_Nucleobase14 rep B; time point 3 +0.3 +0.1 +3.8 +0.0 +0.2
carbon source Inosine 0.3728 mM (C) -0.1 +0.2 +4.6 +0.1 -0.2
carbon source Inosine 0.3728 mM (C) -0.0 -0.2 +5.9 +0.1 -0.0
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