Fitness for 5 genes in Escherichia coli BW25113

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500 ntrfbD and rfbB overlap by 1 nucleotidesrfbB and galF are separated by 372 nucleotidesgalF and wcaM are separated by 174 nucleotideswcaM and wcaL are separated by 10 nucleotides b2040: rfbD - dTDP-4-dehydrorhamnose reductase subunit, NAD(P)-binding, of dTDP-L-rhamnose synthase (NCBI), at 2,109,101 to 2,110,000 rfbD b2041: rfbB - dTDP-glucose 4,6 dehydratase, NAD(P)-binding (NCBI), at 2,110,000 to 2,111,085 rfbB b2042: galF - predicted subunit with GalU (NCBI), at 2,111,458 to 2,112,351 galF b2043: wcaM - predicted colanic acid biosynthesis protein (NCBI), at 2,112,526 to 2,113,920 wcaM b2044: wcaL - predicted glycosyl transferase (NCBI), at 2,113,931 to 2,115,151 wcaL
Group Condition b2040 b2041 b2042 b2043 b2044
rfbD rfbB galF wcaM wcaL
phage T7_phage 0.00015625 MOI TC2 -5.9 -0.9 -0.9 +0.1 +0.4
phage T7_phage 0.000015625 MOI TC1 -6.1 -0.1 -0.1 -0.1 -0.7
phage T7_phage 0.000015625 MOI TC4 -6.3 -0.1 -0.2 +0.1 -0.1
phage T7_phage 0.00015625 MOI TC1 -6.1 -0.2 -0.1 -0.0 -0.2
phage T7_phage 0.000015625 MOI TC3 -6.1 -0.3 -0.4 +0.0 +0.2
phage T7_phage 0.000015625 MOI TC5 -6.4 +0.1 +0.1 -0.0 -0.3
phage T7_phage 0.000015625 MOI TC2 -5.8 -0.1 -0.2 +0.1 -0.2
phage T7_phage_31.25_MOI -6.2 +0.1 +0.2 +0.0 -0.2
phage T7_phage 0.0015625 MOI TC1 -5.0 -0.6 -0.8 +0.1 +0.2
phage phage -5.0 +0.1 -0.0 -0.2 +0.4
stress Benzalkonium Chloride 0.004 mg/ml -3.0 -1.5 -0.0 +0.2 -0.2
phage T7_phage 0.0000015625 MOI TC5 -4.2 +0.0 -0.0 -0.1 +0.0
phage T7_phage 0.00015625 MOI TC4 -4.3 -0.3 -0.5 +0.1 +0.8
phage T7_phage 0.0015625 MOI TC2 -2.0 -0.4 -0.4 -0.1 -0.9
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -1.6 -1.6 +0.4 +0.1 -0.7
control PBS_cage_1_mice _Feces_40 hr -1.5 -0.2 -0.2 -0.0 -1.4
phage T7_phage_3.125_MOI -3.9 +0.2 +0.1 +0.1 +0.1
phage T7_phage 0.00015625 MOI TC3 -1.7 -0.1 -1.0 +0.2 -0.7
phage Laval1374 -0.6 -0.0 +0.2 +0.2 -2.5
phage Laval1374 -0.5 +0.0 +0.1 +0.1 -2.4
control PBS_cage_1_mice _Feces_22 hr -1.1 -0.1 -0.1 -0.1 -1.2
control PBS_cage_1_mice _Feces_16 hr -1.0 -0.1 +0.0 -0.0 -1.4
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr -1.9 +0.1 -0.3 +0.3 -0.4
phage Phage_N4_cage_3_mice _Feces_40 hr -1.3 +0.1 -0.3 +0.2 -0.6
control PBS_cage_2_mouse_3_Cecum_40 hr -1.6 +0.1 +0.4 +0.2 +0.4
carbon source carbon source D-Glucose 0.25mM -0.6 -0.2 -0.7 -0.1 +1.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.8 +0.6 +0.7 -0.2 -0.5
stress Trimethoprim 0.005 mM +1.3 +0.7 +0.5 +0.3 -0.3
stress Trimethoprim 0.0025 mM +2.1 +1.9 +1.1 +0.1 -0.5
stress Trimethoprim 0.00125 mM +2.1 +1.7 +1.0 +0.0 +0.0
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