Fitness for 5 genes in Escherichia coli BW25113

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500 ntrfbA and rfbD are separated by 57 nucleotidesrfbD and rfbB overlap by 1 nucleotidesrfbB and galF are separated by 372 nucleotidesgalF and wcaM are separated by 174 nucleotides b2039: rfbA - glucose-1-phosphate thymidylyltransferase (NCBI), at 2,108,162 to 2,109,043 rfbA b2040: rfbD - dTDP-4-dehydrorhamnose reductase subunit, NAD(P)-binding, of dTDP-L-rhamnose synthase (NCBI), at 2,109,101 to 2,110,000 rfbD b2041: rfbB - dTDP-glucose 4,6 dehydratase, NAD(P)-binding (NCBI), at 2,110,000 to 2,111,085 rfbB b2042: galF - predicted subunit with GalU (NCBI), at 2,111,458 to 2,112,351 galF b2043: wcaM - predicted colanic acid biosynthesis protein (NCBI), at 2,112,526 to 2,113,920 wcaM
Group Condition b2039 b2040 b2041 b2042 b2043
rfbA rfbD rfbB galF wcaM
phage T7_phage 0.00015625 MOI TC2 -0.3 -5.9 -0.9 -0.9 +0.1
phage T7_phage 0.000015625 MOI TC3 -0.0 -6.1 -0.3 -0.4 +0.0
phage T7_phage 0.000015625 MOI TC4 -0.1 -6.3 -0.1 -0.2 +0.1
phage T7_phage 0.00015625 MOI TC1 -0.1 -6.1 -0.2 -0.1 -0.0
phage T7_phage 0.000015625 MOI TC1 -0.0 -6.1 -0.1 -0.1 -0.1
phage T7_phage 0.000015625 MOI TC2 -0.2 -5.8 -0.1 -0.2 +0.1
phage T7_phage 0.000015625 MOI TC5 -0.0 -6.4 +0.1 +0.1 -0.0
phage T7_phage 0.0015625 MOI TC1 +0.2 -5.0 -0.6 -0.8 +0.1
phage T7_phage_31.25_MOI +0.1 -6.2 +0.1 +0.2 +0.0
phage phage +0.2 -5.0 +0.1 -0.0 -0.2
phage T7_phage 0.0000015625 MOI TC5 -0.1 -4.2 +0.0 -0.0 -0.1
phage T7_phage 0.00015625 MOI TC4 +0.8 -4.3 -0.3 -0.5 +0.1
phage T7_phage_3.125_MOI -0.0 -3.9 +0.2 +0.1 +0.1
stress Benzalkonium Chloride 0.004 mg/ml +0.9 -3.0 -1.5 -0.0 +0.2
phage T7_phage 0.0015625 MOI TC2 +0.2 -2.0 -0.4 -0.4 -0.1
phage T7_phage 0.00015625 MOI TC3 +0.3 -1.7 -0.1 -1.0 +0.2
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr -0.1 -1.9 +0.1 -0.3 +0.3
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.9 -1.6 -1.6 +0.4 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.6 +0.4 +0.1 -0.4 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.9 +0.4 +0.3 +0.1 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.7 +0.6 +0.1 +0.1 -0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.8 +0.2 +0.3 +0.5 -0.1
control PBS_cage_2_mouse_3_Cecum_40 hr +0.1 -1.6 +0.1 +0.4 +0.2
stress octanol 0.08 vol% +1.5 -1.2 -0.2 +0.4 -0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.2 +0.5 +0.5 +0.4 -0.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.7 +0.8 +0.6 +0.7 -0.2
stress Bacitracin 1 mg/ml +1.4 -0.4 -0.9 +1.0 -0.0
stress Trimethoprim 0.00125 mM -2.6 +2.1 +1.7 +1.0 +0.0
stress Trimethoprim 0.005 mM -0.6 +1.3 +0.7 +0.5 +0.3
stress Trimethoprim 0.0025 mM -2.4 +2.1 +1.9 +1.1 +0.1
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