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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
rfbX and rfbC overlap by 4 nucleotides
rfbC and rfbA overlap by 1 nucleotides
rfbA and rfbD are separated by 57 nucleotides
rfbD and rfbB overlap by 1 nucleotides
b2037: rfbX - predicted polisoprenol-linked O-antigen transporter (NCBI), at 2,106,361 to 2,107,608
rfbX
b2038: rfbC - dTDP-4-deoxyrhamnose-3,5-epimerase (NCBI), at 2,107,605 to 2,108,162
rfbC
b2039: rfbA - glucose-1-phosphate thymidylyltransferase (NCBI), at 2,108,162 to 2,109,043
rfbA
b2040: rfbD - dTDP-4-dehydrorhamnose reductase subunit, NAD(P)-binding, of dTDP-L-rhamnose synthase (NCBI), at 2,109,101 to 2,110,000
rfbD
b2041: rfbB - dTDP-glucose 4,6 dehydratase, NAD(P)-binding (NCBI), at 2,110,000 to 2,111,085
rfbB
Group
Condition
b2037
b2038
b2039
b2040
b2041
rfbX
rfbC
rfbA
rfbD
rfbB
phage
T7_phage 0.00015625 MOI TC2
-4.6
-6.5
-0.3
-5.9
-0.9
phage
T7_phage 0.000015625 MOI TC3
-1.4
-6.4
-0.0
-6.1
-0.3
phage
T7_phage 0.000015625 MOI TC2
-1.0
-6.4
-0.2
-5.8
-0.1
phage
T7_phage 0.0015625 MOI TC1
-2.5
-5.4
+0.2
-5.0
-0.6
phage
T7_phage 0.00015625 MOI TC1
-0.6
-6.3
-0.1
-6.1
-0.2
phage
T7_phage 0.000015625 MOI TC4
-0.5
-6.1
-0.1
-6.3
-0.1
phage
T7_phage 0.000015625 MOI TC5
-0.2
-6.5
-0.0
-6.4
+0.1
phage
T7_phage 0.000015625 MOI TC1
-0.4
-6.3
-0.0
-6.1
-0.1
phage
T7_phage_31.25_MOI
+0.1
-6.6
+0.1
-6.2
+0.1
phage
T7_phage 0.00015625 MOI TC4
-2.6
-4.8
+0.8
-4.3
-0.3
phage
phage
-0.4
-5.4
+0.2
-5.0
+0.1
phage
T7_phage_3.125_MOI
+0.0
-5.9
-0.0
-3.9
+0.2
phage
T7_phage 0.0000015625 MOI TC5
-0.2
-4.7
-0.1
-4.2
+0.0
phage
T7_phage 0.0015625 MOI TC2
-1.1
-3.6
+0.2
-2.0
-0.4
stress
Benzalkonium Chloride 0.004 mg/ml
+1.5
-2.8
+0.9
-3.0
-1.5
phage
T7_phage 0.00015625 MOI TC3
-1.0
-2.4
+0.3
-1.7
-0.1
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.6
-1.5
+0.9
-1.6
-1.6
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
-0.5
-2.0
-0.2
-1.4
+0.1
phage
Phage_N4_cage_3_mouse_1_Cecum_40 hr
-0.1
-2.0
-0.1
-1.9
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-2.9
+0.7
-1.7
+0.6
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-2.3
+0.3
-1.9
+0.4
+0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-2.7
+0.7
-1.6
+0.4
+0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-2.0
+1.0
-1.8
+0.2
+0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-2.3
+0.6
-1.2
+0.5
+0.5
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.9
+1.0
-1.7
+0.8
+0.6
stress
octanol 0.08 vol%
+0.7
-1.6
+1.5
-1.2
-0.2
stress
Trimethoprim 0.00125 mM
-3.4
+1.5
-2.6
+2.1
+1.7
stress
Spectinomycin 0.0125 mg/ml
-2.1
+1.0
-0.3
+0.6
+0.2
stress
Trimethoprim 0.0025 mM
-2.7
+1.4
-2.4
+2.1
+1.9
stress
Bacitracin 1 mg/ml
+1.6
-0.6
+1.4
-0.4
-0.9
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