Fitness for 5 genes in Escherichia coli BW25113

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500 ntfliF and fliG overlap by 8 nucleotidesfliG and fliH overlap by 8 nucleotidesfliH and fliI overlap by 1 nucleotidesfliI and fliJ are separated by 18 nucleotides b1938: fliF - flagellar M-ring protein (NCBI), at 2,011,253 to 2,012,911 fliF b1939: fliG - flagellar motor switch protein G (NCBI), at 2,012,904 to 2,013,899 fliG b1940: fliH - flagellar biosynthesis; export of flagellar proteins? (VIMSS), at 2,013,892 to 2,014,578 fliH b1941: fliI - flagellum-specific ATP synthase (NCBI), at 2,014,578 to 2,015,951 fliI b1942: fliJ - flagellar biosynthesis chaperone (NCBI), at 2,015,970 to 2,016,413 fliJ
Group Condition b1938 b1939 b1940 b1941 b1942
fliF fliG fliH fliI fliJ
motility outer cut, LB soft agar motility assay -6.1 -7.4 -5.4 -4.8 -3.8
motility outer cut, LB soft agar motility assay -2.9 -1.7 -2.5 -2.0 -4.1
motility inner cut, LB soft agar motility assay -0.5 -1.0 -1.1 -2.0 -1.0
carbon source carbon source D-Glucose 0.25mM +0.7 -0.7 +0.1 -0.8 -4.8
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.0 -0.4 -0.3 -0.1 -4.6
carbon source carbon source D-Glucose 20mM +0.5 -0.0 -0.9 -0.1 -4.9
lb LB, Anaerobic -0.9 -1.4 -0.7 -0.6 -1.7
carbon source carbon source D-Mannose 0.5mM +0.3 -0.0 -0.4 +0.0 -4.8
carbon source carbon source D-Glucose 5mM +0.2 -0.3 +0.3 -0.2 -4.6
stress LB Anaerobic with Zinc Pyrithione 0.0000025 mM -1.1 -0.8 -1.2 -0.4 -1.1
stress LB Anaerobic with Norfloxacin 0.0000000390625 mM -0.5 -0.6 -0.5 -0.8 -2.2
phage T7_phage 0.00015625 MOI TC3 -0.8 -1.8 -1.0 -0.7 -0.1
ecofab LB in EcoFAB_3.5mL -1.2 -0.6 -1.4 -0.9 -0.2
control PBS_cage_2_mouse_3_Cecum_40 hr +0.2 +0.2 +0.1 -0.3 -4.5
phage T7_phage 0.0015625 MOI TC2 -0.0 -0.1 -0.4 -1.1 -2.7
motility inner cut, LB soft agar motility assay -0.2 -1.5 -0.9 -0.9 -0.7
motility inner cut, LB soft agar motility assay -0.7 -0.7 -1.1 -2.2 +0.7
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.6 -1.3 -0.9 -0.7 -0.3
stress LB Anaerobic with Sulfamonomethoxine 0.00003125 mM -0.6 -1.4 -0.8 -0.7 -0.2
stress Aluminum chloride 5 mM -0.1 -1.1 +0.2 -0.2 -2.4
motility inner cut, LB soft agar motility assay -0.7 -0.7 -1.2 -1.6 +1.2
phage Ste41 +0.4 -0.9 +0.1 -0.4 -2.0
motility outer cut, LB soft agar motility assay -1.9 -0.4 -0.7 -1.3 +1.6
stress 3-methyl-1-butanol 1.25 vol% -0.1 +0.5 -0.2 +0.2 -2.7
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.4 -1.5 -0.6 -0.4 +0.9
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.9 -1.3 -0.3 -0.9 +0.2
carbon source carbon source D-Glucose 1mM +0.5 -0.1 +0.7 -0.1 -2.1
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.6 -0.9 -1.0 -0.2 +1.4
phage Ste41 -0.2 -1.4 +0.1 +0.2 +1.5
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.2 +0.4 +1.0 +0.4 +1.8
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