Fitness for 5 genes in Escherichia coli BW25113

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500 ntftn and yecH are separated by 37 nucleotidesyecH and tyrP are separated by 190 nucleotidestyrP and yecA are separated by 61 nucleotidesyecA and leuZ are separated by 195 nucleotides b1905: ftn - ferritin iron storage protein (cytoplasmic) (NCBI), at 1,986,740 to 1,987,237 ftn b1906: yecH - hypothetical protein (NCBI), at 1,987,275 to 1,987,514 yecH b1907: tyrP - tyrosine transporter (NCBI), at 1,987,705 to 1,988,916 tyrP b1908: yecA - conserved metal-binding protein (NCBI), at 1,988,978 to 1,989,643 yecA b1909: leuZ - tRNA-Leu (NCBI), at 1,989,839 to 1,989,925 leuZ
Group Condition b1905 b1906 b1907 b1908 b1909
ftn yecH tyrP yecA leuZ
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -0.5 N.D. -2.4 -1.4 N.D.
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.2 N.D. -3.4 +0.2 N.D.
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -0.5 N.D. -1.3 -1.4 N.D.
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM -1.1 N.D. -1.4 -0.7 N.D.
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -1.4 N.D. -1.3 -0.4 N.D.
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.0 N.D. -3.1 +0.2 N.D.
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.5 N.D. -1.4 -0.8 N.D.
M9 Acetate 0.5% aerobic -1.0 N.D. -1.0 -0.5 N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.8 N.D. -1.4 -0.3 N.D.
phage Δdbn_10 +0.3 N.D. -1.6 -1.1 N.D.
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -1.2 N.D. -1.3 +0.2 N.D.
carbon source Glycolic Acid (C) -1.3 N.D. -0.9 -0.2 N.D.
carbon source Glycolic Acid (C) -1.1 N.D. -1.0 -0.2 N.D.
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.4 N.D. -1.0 -0.9 N.D.
stress LB Anaerobic with Norfloxacin 0.0000000390625 mM -0.6 N.D. -0.9 -0.7 N.D.
stress LB Aerobic with Benzethonium chloride 0.000015625 mM -1.0 N.D. -0.3 -0.8 N.D.
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -0.4 N.D. -0.9 -0.8 N.D.
carbon source carbon source D-Glucose 0.25mM -0.1 N.D. -0.3 -1.5 N.D.
M9 Acetate 0.5% aerobic -1.0 N.D. -0.6 -0.4 N.D.
stress LB Aerobic with Novobiocin 0.0000625 mM -0.6 N.D. -0.8 -0.5 N.D.
stress LB Anaerobic with Irgasan 0.000000625 mM -0.8 N.D. -0.3 -0.9 N.D.
stress LB Aerobic with 8-hydroxy-nitroquinoline 0.0000025 mM -0.5 N.D. -0.7 -0.8 N.D.
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -1.0 N.D. -0.9 +0.3 N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.6 N.D. -1.2 +0.2 N.D.
stress Cisplatin 0.1 mg/ml +0.2 N.D. -1.6 +0.1 N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.2 N.D. -1.3 +1.0 N.D.
control PBS_cage_2_mouse_2_Cecum_40 hr -0.4 N.D. -0.6 +1.4 N.D.
control PBS_cage_2_mice _Feces_40 hr -0.2 N.D. -0.6 +1.6 N.D.
control PBS_cage_2_mouse_1_Cecum_40 hr -0.5 N.D. -0.0 +1.5 N.D.
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.5 N.D. +0.3 +1.3 N.D.
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