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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
cheA and motB are separated by 4 nucleotides
motB and motA overlap by 4 nucleotides
motA and flhC are separated by 126 nucleotides
flhC and flhD are separated by 2 nucleotides
b1888: cheA - fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein (NCBI), at 1,971,384 to 1,973,348
cheA
b1889: motB - flagellar motor protein MotB (NCBI), at 1,973,353 to 1,974,279
motB
b1890: motA - flagellar motor protein MotA (NCBI), at 1,974,276 to 1,975,163
motA
b1891: flhC - DNA-binding transcriptional dual regulator with FlhD (NCBI), at 1,975,290 to 1,975,868
flhC
b1892: flhD - DNA-binding transcriptional dual regulator with FlhC (RefSeq), at 1,975,871 to 1,976,221
flhD
Group
Condition
b1888
b1889
b1890
b1891
b1892
cheA
motB
motA
flhC
flhD
motility
outer cut, LB soft agar motility assay
-5.3
-6.4
-6.7
-1.1
-6.0
motility
outer cut, LB soft agar motility assay
-1.8
-0.7
-2.0
-1.3
-0.4
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-0.9
-1.9
-1.2
-1.0
-0.8
motility
inner cut, LB soft agar motility assay
-1.2
-1.7
+0.4
-2.2
-0.8
ecofab
LB in EcoFAB_3.5mL
-0.9
-1.1
-1.0
-1.1
-1.3
motility
inner cut, LB soft agar motility assay
-1.0
-0.0
-1.2
-1.4
-1.2
motility
inner cut, LB soft agar motility assay
-1.2
-1.2
+0.3
-1.3
-1.4
motility
inner cut, LB soft agar motility assay
-0.6
-0.4
-1.1
-1.4
-0.9
stress
LB Anaerobic with Norfloxacin 0.0000000390625 mM
-0.8
-0.8
-0.8
-1.0
-0.9
lb
LB, Anaerobic
-0.6
-1.0
-0.9
-0.6
-1.2
stress
LB Anaerobic with Zinc Pyrithione 0.0000025 mM
-0.6
-1.0
-0.7
-0.9
-1.2
stress
LB Anaerobic with Sulfamonomethoxine 0.00003125 mM
-0.6
-1.0
-1.1
-0.5
-0.9
motility
outer cut, LB soft agar motility assay
-0.5
-0.9
-1.3
-0.4
-0.7
ecofab
LB 0.5x in EcoFAB_3.5mL
-0.7
-0.4
-1.0
-0.7
-0.9
phage
HSDP4
-0.2
+0.4
-1.5
-0.4
-1.0
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.1
-1.2
-0.2
-0.7
-0.5
stress
LB Anaerobic with Sulfamonomethoxine 0.0000625 mM
-0.3
-0.7
-0.6
-0.5
-0.4
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-0.6
-0.6
+0.2
-0.3
-0.9
phage
T7_phage 0.00015625 MOI TC4
+0.5
-0.5
-0.8
-0.7
+0.5
phage
T7_phage 0.0015625 MOI TC2
-0.1
-1.4
-0.1
+0.6
+0.3
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.0
-0.1
+0.7
+0.2
-1.5
phage
Ste41
+0.4
-0.2
+0.5
-1.1
+0.4
phage
Phage_N4_cage_3_mouse_1_Cecum_40 hr
+0.6
-0.7
+0.7
-0.4
+0.4
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.2
-0.6
+0.3
+1.2
+0.8
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.6
-0.0
-0.5
+0.8
+1.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.3
+0.1
+0.9
+1.0
-0.3
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.6
+0.4
+0.2
+1.4
+1.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.3
-0.1
+0.4
+1.2
+0.8
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.6
-0.5
+0.7
+1.5
+0.8
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.2
-0.4
+0.7
+1.7
+1.1
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