Fitness for 5 genes in Escherichia coli BW25113

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500 ntcheA and motB are separated by 4 nucleotidesmotB and motA overlap by 4 nucleotidesmotA and flhC are separated by 126 nucleotidesflhC and flhD are separated by 2 nucleotides b1888: cheA - fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein (NCBI), at 1,971,384 to 1,973,348 cheA b1889: motB - flagellar motor protein MotB (NCBI), at 1,973,353 to 1,974,279 motB b1890: motA - flagellar motor protein MotA (NCBI), at 1,974,276 to 1,975,163 motA b1891: flhC - DNA-binding transcriptional dual regulator with FlhD (NCBI), at 1,975,290 to 1,975,868 flhC b1892: flhD - DNA-binding transcriptional dual regulator with FlhC (RefSeq), at 1,975,871 to 1,976,221 flhD
Group Condition b1888 b1889 b1890 b1891 b1892
cheA motB motA flhC flhD
motility outer cut, LB soft agar motility assay -5.3 -6.4 -6.7 -1.1 -6.0
motility outer cut, LB soft agar motility assay -1.8 -0.7 -2.0 -1.3 -0.4
stress LB Anaerobic with Cephalothin 0.0000078125 mM -0.9 -1.9 -1.2 -1.0 -0.8
motility inner cut, LB soft agar motility assay -1.2 -1.7 +0.4 -2.2 -0.8
ecofab LB in EcoFAB_3.5mL -0.9 -1.1 -1.0 -1.1 -1.3
motility inner cut, LB soft agar motility assay -1.0 -0.0 -1.2 -1.4 -1.2
motility inner cut, LB soft agar motility assay -1.2 -1.2 +0.3 -1.3 -1.4
motility inner cut, LB soft agar motility assay -0.6 -0.4 -1.1 -1.4 -0.9
stress LB Anaerobic with Norfloxacin 0.0000000390625 mM -0.8 -0.8 -0.8 -1.0 -0.9
lb LB, Anaerobic -0.6 -1.0 -0.9 -0.6 -1.2
stress LB Anaerobic with Zinc Pyrithione 0.0000025 mM -0.6 -1.0 -0.7 -0.9 -1.2
stress LB Anaerobic with Sulfamonomethoxine 0.00003125 mM -0.6 -1.0 -1.1 -0.5 -0.9
motility outer cut, LB soft agar motility assay -0.5 -0.9 -1.3 -0.4 -0.7
ecofab LB 0.5x in EcoFAB_3.5mL -0.7 -0.4 -1.0 -0.7 -0.9
phage HSDP4 -0.2 +0.4 -1.5 -0.4 -1.0
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.1 -1.2 -0.2 -0.7 -0.5
stress LB Anaerobic with Sulfamonomethoxine 0.0000625 mM -0.3 -0.7 -0.6 -0.5 -0.4
stress LB Anaerobic with Furaltadone 0.000015625 mM -0.6 -0.6 +0.2 -0.3 -0.9
phage T7_phage 0.00015625 MOI TC4 +0.5 -0.5 -0.8 -0.7 +0.5
phage T7_phage 0.0015625 MOI TC2 -0.1 -1.4 -0.1 +0.6 +0.3
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.0 -0.1 +0.7 +0.2 -1.5
phage Ste41 +0.4 -0.2 +0.5 -1.1 +0.4
phage Phage_N4_cage_3_mouse_1_Cecum_40 hr +0.6 -0.7 +0.7 -0.4 +0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.2 -0.6 +0.3 +1.2 +0.8
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.6 -0.0 -0.5 +0.8 +1.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 +0.1 +0.9 +1.0 -0.3
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.6 +0.4 +0.2 +1.4 +1.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.3 -0.1 +0.4 +1.2 +0.8
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.6 -0.5 +0.7 +1.5 +0.8
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.2 -0.4 +0.7 +1.7 +1.1
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