Fitness for 5 genes in Escherichia coli BW25113

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500 ntyecN and yecO are separated by 40 nucleotidesyecO and yecP overlap by 4 nucleotidesyecP and bisZ are separated by 164 nucleotidesbisZ and torY are separated by 24 nucleotides b1869: yecN - orf, hypothetical protein (VIMSS), at 1,950,290 to 1,950,685 yecN b1870: yecO - predicted methyltransferase (NCBI), at 1,950,726 to 1,951,469 yecO b1871: yecP - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 1,951,466 to 1,952,437 yecP b1872: bisZ - biotin sulfoxide reductase 2 (VIMSS), at 1,952,602 to 1,955,031 bisZ b1873: torY - TMAO reductase III (TorYZ), cytochrome c-type subunit (NCBI), at 1,955,056 to 1,956,156 torY
Group Condition b1869 b1870 b1871 b1872 b1873
yecN yecO yecP bisZ torY
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.1 N.D. -1.0 -0.7 -0.8
phage RB68 -0.4 N.D. -1.1 -0.4 -0.3
carbon source acetate (C) -0.5 N.D. -1.6 -0.0 -0.0
protonated/deuterated deuterated glucose in D2O -0.4 N.D. -1.5 -0.1 -0.1
carbon source acetate (C) -0.6 N.D. -1.5 -0.1 +0.1
phage RB68 -0.1 N.D. -1.3 -0.5 -0.1
carbon source pyruvate (C) -0.3 N.D. -1.6 +0.0 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.4 N.D. -1.2 -0.1 -0.2
phage Ste41 +0.2 N.D. -1.4 -0.6 -0.1
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.0 N.D. -1.4 -0.1 -0.3
sulfur source M63 no sulfur + 5 mM sulfate -0.1 N.D. -1.5 -0.0 -0.1
stress LB Aerobic with Irgasan 0.0000003125 mM -0.6 N.D. -1.1 +0.1 -0.1
stress LB Aerobic with Nitrofurantoin 0.00003125 mM -0.4 N.D. -1.1 +0.2 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.1 N.D. -1.6 +0.4 -0.3
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.2 N.D. -1.5 +0.3 -0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.6 N.D. -0.8 -0.2 +0.1
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.2 N.D. -1.6 -0.0 +0.0
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.4 N.D. -1.3 -0.1 -0.3
predation 6 hr rep2_HD100_10fold_dilution_grewON +0.1 N.D. -1.4 -0.1 +0.2
sulfur source M63 no sulfur + 5 mM L-cys +0.3 N.D. -1.6 +0.1 +0.1
msc 3hr_MSC_rep2_lowinoculum -1.0 N.D. +0.5 -0.2 -0.2
control PBS_cage_2_mouse_1_Cecum_40 hr -0.3 N.D. -1.1 +0.1 +0.5
phage T7_phage 0.0015625 MOI TC2 -1.0 N.D. +0.8 +0.1 -0.5
stress LB Aerobic with Ramoplanin 0.000125 mM +0.5 N.D. -1.1 +0.2 +0.1
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.6 N.D. -0.5 +0.0 +0.8
phage T7_phage 0.00015625 MOI TC4 +0.7 N.D. -0.5 +0.4 -0.1
carbon source carbon source D-Mannose 1mM -0.2 N.D. +0.4 -0.4 +0.7
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.7 N.D. +0.9 -0.6 -0.3
stress Trimethoprim 0.005 mM -0.0 N.D. +0.6 -0.3 +0.8
stress Benzalkonium Chloride 0.004 mg/ml +0.3 N.D. +1.3 +0.1 +0.2
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