Fitness for 5 genes in Escherichia coli BW25113

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500 ntnudB and aspS are separated by 117 nucleotidesaspS and yecD are separated by 309 nucleotidesyecD and yecE overlap by 4 nucleotidesyecE and yecN are separated by 52 nucleotides b1865: nudB - dATP pyrophosphohydrolase (NCBI), at 1,946,204 to 1,946,656 nudB b1866: aspS - aspartyl-tRNA synthetase (NCBI), at 1,946,774 to 1,948,546 aspS b1867: yecD - orf, hypothetical protein (VIMSS), at 1,948,856 to 1,949,422 yecD b1868: yecE - hypothetical protein (NCBI), at 1,949,419 to 1,950,237 yecE b1869: yecN - orf, hypothetical protein (VIMSS), at 1,950,290 to 1,950,685 yecN
Group Condition b1865 b1866 b1867 b1868 b1869
nudB aspS yecD yecE yecN
carbon source Ying_Nucleobase14 rep A; time point 2 -7.4 N.D. +0.1 +0.2 -0.5
gfp_control GFP_control -7.2 N.D. +0.1 -0.4 +0.1
carbon source Ying_sugar7 rep A; time point 3 -7.2 N.D. +0.2 -0.2 -0.3
mzaabcde mzaABCDE -7.0 N.D. +0.2 +0.1 -0.7
mzaabcde mzaABCDE -6.9 N.D. -0.2 -0.5 +0.2
carbon source Ying_sugar7 rep C; time point 3 -7.1 N.D. +0.3 -0.1 -0.4
carbon source Ying_Nucleobase14 rep A; time point 3 -7.1 N.D. +0.1 +0.2 -0.3
carbon source Ying_sugar7 rep B; time point 2 -7.3 N.D. +0.1 +0.2 -0.2
carbon source Ying_OrganicAcid7 rep A; time point 2 -7.4 N.D. +0.3 -0.0 -0.0
carbon source Ying_sugar7 rep B; time point 3 -7.2 N.D. +0.3 +0.2 -0.2
mzaabcde mzaABCDE -6.9 N.D. -0.3 +0.2 +0.1
carbon source carbon source D-Glucose 0.25mM -5.7 N.D. -0.0 -0.9 +0.0
carbon source carbon source D-Glucose 0.5mM -6.4 N.D. -0.2 -0.1 +0.2
motility outer cut, LB soft agar motility assay -6.3 N.D. +0.6 -0.3 -0.5
carbon source carbon source D-Glucose 20mM -6.1 N.D. -0.3 +0.5 -0.5
carbon source carbon source D-Mannose 1mM -6.1 N.D. -0.3 +0.0 +0.1
predation 6 hr rep1_HD100_10fold_dilution_grewON -6.0 N.D. +0.4 -0.1 -0.6
carbon source carbon source D-Mannose 1mM -5.8 N.D. +0.2 -0.4 -0.2
control PBS_cage_2_mouse_1_Cecum_40 hr -6.8 N.D. +0.4 +0.6 -0.3
carbon source carbon source D-Glucose 20mM -6.1 N.D. -0.0 +0.1 +0.0
carbon source carbon source D-Glucose 5mM -5.6 N.D. +0.6 +0.0 -0.3
carbon source carbon source D-Mannose 0.5mM -5.8 N.D. +0.5 +0.2 -0.0
carbon source carbon source D-Mannose 5mM -5.4 N.D. +0.2 -0.1 +0.3
carbon source Ying_Nucleobase14 rep C; time point 3 -5.1 N.D. +0.1 +0.3 -0.2
carbon source Ying_Nucleobase14 rep C; time point 2 -5.1 N.D. +0.3 +0.2 -0.3
carbon source succinate (C) -4.8 N.D. +0.4 +0.4 -0.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM +4.5 N.D. +0.4 -0.9 -0.1
phage T7_phage 0.0015625 MOI TC2 +4.8 N.D. +0.3 +0.7 -1.0
stress Cephalothin 0.01 mg/ml +5.9 N.D. +0.1 +0.0 +0.3
stress Benzalkonium Chloride 0.004 mg/ml +6.3 N.D. +0.0 -0.1 +0.3
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