Fitness for 5 genes in Escherichia coli BW25113

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500 ntmanZ and yobD are separated by 54 nucleotidesyobD and b1821 are separated by 428 nucleotidesb1821 and rrmA overlap by 4 nucleotidesrrmA and cspC are separated by 165 nucleotides b1819: manZ - mannose-specific enzyme IID component of PTS (NCBI), at 1,901,919 to 1,902,770 manZ b1820: yobD - hypothetical protein (NCBI), at 1,902,825 to 1,903,283 yobD b1821: b1821 - orf, hypothetical protein (VIMSS), at 1,903,712 to 1,904,278 b1821 b1822: rrmA - 23S rRNA m1G745 methyltransferase (NCBI), at 1,904,275 to 1,905,084 rrmA b1823: cspC - stress protein, member of the CspA-family (NCBI), at 1,905,250 to 1,905,459 cspC
Group Condition b1819 b1820 b1821 b1822 b1823
manZ yobD b1821 rrmA cspC
carbon source carbon source D-Mannose 1mM -5.6 -1.3 -0.1 +0.5 -5.7
carbon source carbon source D-Mannose 1mM -7.1 -0.8 -0.3 -0.1 +1.2
carbon source carbon source D-Mannose 0.5mM -5.4 -1.0 +0.1 +0.0 -0.4
carbon source carbon source D-Mannose 0.5mM -6.8 -0.9 -0.4 +1.0 +1.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.5 -0.3 -0.4 -0.4 -4.3
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.3 -0.1 +0.4 +0.5 -5.0
carbon source D-Glucosamine Hydrochloride (C) -4.1 -0.5 +0.1 -0.2 +0.8
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.0 -0.0 +0.9 +0.2 -5.0
phage R1_N4_T2_phage8 +0.4 -0.2 +0.4 -0.5 -4.0
carbon source Ying_sugar7 rep C; time point 3 +0.5 +0.1 -4.3 -0.2 +1.0
carbon source Ying_sugar7 rep A; time point 3 +0.3 +0.4 -3.9 +0.0 +1.5
carbon source Ying_sugar7 rep B; time point 3 +0.2 +0.2 -3.5 +0.2 +1.4
carbon source Ying_AminoAcid20 rep B; time point 3 -0.1 +0.2 -3.3 +0.9 +1.2
carbon source Ying_AminoAcid20 rep C; time point 3 +0.2 +0.2 -2.4 +1.0 +1.8
carbon source Ying_AminoAcid20 rep A; time point 3 +0.5 -0.0 -2.7 +1.3 +1.9
carbon source L-Malic (C) -0.5 +0.1 +0.3 +1.5 +3.4
carbon source succinate (C) -0.2 -0.1 +0.6 +1.6 +3.0
M9 Acetate 0.5% aerobic +0.2 -0.1 +0.1 +1.3 +3.4
M9 Acetate 0.5% aerobic -0.2 -0.0 +0.1 +1.7 +3.5
carbon source a-Ketoglutaric (C) +0.2 +0.1 +0.2 +1.7 +2.9
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) +0.3 +0.4 +0.1 +1.1 +3.3
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) +0.4 +0.4 -0.2 +1.2 +3.4
carbon source L-Malic (C) +0.4 +0.0 +0.2 +1.6 +3.5
carbon source acetate (C) +0.2 +0.0 +0.6 +1.7 +3.2
nitrogen source L-Arginine (N) +0.3 -0.1 +0.4 +1.8 +4.0
nitrogen source L-Arginine (N) +0.4 +0.1 +0.4 +2.0 +3.9
nitrogen source Gly-Glu (N) -0.1 +0.1 +0.3 +1.0 +5.5
nitrogen source Gly-Glu (N) +0.3 +0.1 +0.3 +1.2 +5.4
carbon source Acetate (C) -0.3 -0.2 +0.5 +2.3 +5.7
carbon source Acetate (C) -0.2 -0.3 +0.2 +2.3 +5.8
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