Fitness for 5 genes in Escherichia coli BW25113

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500 ntmanX and manY are separated by 62 nucleotidesmanY and manZ are separated by 12 nucleotidesmanZ and yobD are separated by 54 nucleotidesyobD and b1821 are separated by 428 nucleotides b1817: manX - fused mannose-specific PTS enzymes: IIA component/IIB component (NCBI), at 1,900,072 to 1,901,043 manX b1818: manY - mannose-specific enzyme IIC component of PTS (NCBI), at 1,901,106 to 1,901,906 manY b1819: manZ - mannose-specific enzyme IID component of PTS (NCBI), at 1,901,919 to 1,902,770 manZ b1820: yobD - hypothetical protein (NCBI), at 1,902,825 to 1,903,283 yobD b1821: b1821 - orf, hypothetical protein (VIMSS), at 1,903,712 to 1,904,278 b1821
Group Condition b1817 b1818 b1819 b1820 b1821
manX manY manZ yobD b1821
carbon source carbon source D-Mannose 0.5mM -7.8 -7.0 -6.8 -0.9 -0.4
carbon source carbon source D-Mannose 1mM -5.3 -7.4 -7.1 -0.8 -0.3
carbon source carbon source D-Mannose 0.5mM -3.4 -7.5 -5.4 -1.0 +0.1
carbon source carbon source D-Mannose 1mM -3.9 -4.7 -5.6 -1.3 -0.1
carbon source D-Glucosamine Hydrochloride (C) -3.9 -3.8 -4.1 -0.5 +0.1
carbon source carbon source D-Mannose 20mM -1.4 -7.6 -1.6 +0.1 +0.5
carbon source D-Mannose (C) -3.1 -3.6 -2.5 -0.4 +0.4
carbon source carbon source D-Mannose 5mM -4.5 -0.1 -3.5 -0.2 -0.9
carbon source D-Glucosamine Hydrochloride (C) -3.7 -1.8 -3.1 -0.6 +0.4
carbon source D-Mannose (C) -2.4 -2.4 -3.0 -0.4 -0.3
carbon source carbon source D-Mannose 5mM -3.5 -1.2 -2.9 -0.3 +0.2
carbon source carbon source D-Mannose 20mM -1.3 -2.2 -1.6 -0.0 -0.8
phage N4_phage 0.009375 MOI TC2 +0.0 -2.0 -0.8 -0.4 -1.8
stress Trimethoprim 0.005 mM -0.9 -1.3 -1.0 -0.3 -1.5
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.7 -3.6 -0.7 -0.3 -1.0
stress Vanillin 5 mM -0.5 -3.3 -0.6 +0.3 -0.5
phage Δdbn_10 -0.8 -3.1 -0.9 +0.1 +0.2
control PBS_cage_2_mouse_1_Cecum_40 hr -1.5 -0.9 -1.2 -0.6 -0.2
control PBS_cage_2_mouse_2_Cecum_40 hr -1.3 -0.6 -0.8 -0.6 -0.8
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -0.8 -1.8 -1.0 -0.8 +0.2
stress LB Anaerobic with Amoxicillin 0.0000078125 mM -0.5 -3.0 -0.3 -0.3 +0.2
stress Chlorite 1.25 mM -1.5 -0.9 -1.1 -0.8 +0.3
phage Phage_N4_cage_4_mouse_1_Cecum_40 hr -1.0 -1.7 -1.1 -0.1 +0.2
carbon source Ying_sugar7 rep C; time point 3 +0.0 +0.4 +0.5 +0.1 -4.3
carbon source Ying_sugar7 rep A; time point 3 +0.1 +0.1 +0.3 +0.4 -3.9
carbon source Ying_sugar7 rep B; time point 3 -0.1 +0.3 +0.2 +0.2 -3.5
carbon source Ying_sugar7 rep C; time point 2 +0.1 +0.1 +0.4 +0.2 -3.5
stress Ciprofloxacin Hydrochloride 0.0024 mM +0.2 -2.7 -0.2 +0.2 +0.8
carbon source Ying_AminoAcid20 rep A; time point 3 +0.1 +0.7 +0.5 -0.0 -2.7
nophagecontrol R1_N4_T1_phage0 +0.6 +1.5 +0.1 +0.3 +1.5
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