Fitness for 5 genes in Escherichia coli BW25113

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500 ntb1815 and yoaE are separated by 3 nucleotidesyoaE and manX are separated by 462 nucleotidesmanX and manY are separated by 62 nucleotidesmanY and manZ are separated by 12 nucleotides b1815: b1815 - orf, hypothetical protein (VIMSS), at 1,896,451 to 1,898,049 b1815 b1816: yoaE - fused predicted membrane protein/conserved protein (NCBI), at 1,898,053 to 1,899,609 yoaE b1817: manX - fused mannose-specific PTS enzymes: IIA component/IIB component (NCBI), at 1,900,072 to 1,901,043 manX b1818: manY - mannose-specific enzyme IIC component of PTS (NCBI), at 1,901,106 to 1,901,906 manY b1819: manZ - mannose-specific enzyme IID component of PTS (NCBI), at 1,901,919 to 1,902,770 manZ
Group Condition b1815 b1816 b1817 b1818 b1819
b1815 yoaE manX manY manZ
carbon source carbon source D-Mannose 0.5mM -0.1 +0.2 -7.8 -7.0 -6.8
carbon source carbon source D-Mannose 1mM +0.3 +0.4 -5.3 -7.4 -7.1
carbon source carbon source D-Mannose 0.5mM +0.2 +0.5 -3.4 -7.5 -5.4
carbon source carbon source D-Mannose 1mM +1.2 +0.1 -3.9 -4.7 -5.6
carbon source D-Glucosamine Hydrochloride (C) +0.3 +0.0 -3.9 -3.8 -4.1
carbon source carbon source D-Mannose 20mM +0.4 +0.4 -1.4 -7.6 -1.6
carbon source D-Mannose (C) +0.0 -0.0 -3.1 -3.6 -2.5
carbon source D-Glucosamine Hydrochloride (C) +0.2 -0.1 -3.7 -1.8 -3.1
carbon source D-Mannose (C) -0.0 +0.2 -2.4 -2.4 -3.0
carbon source carbon source D-Mannose 5mM +0.3 +0.4 -4.5 -0.1 -3.5
carbon source carbon source D-Mannose 5mM +0.3 +0.2 -3.5 -1.2 -2.9
phage Δdbn_10 -0.0 -0.2 -0.8 -3.1 -0.9
carbon source carbon source D-Mannose 20mM +0.2 +0.1 -1.3 -2.2 -1.6
stress LB Anaerobic with Amoxicillin 0.0000078125 mM -0.2 +0.0 -0.5 -3.0 -0.3
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM -0.3 -0.3 -0.3 -2.7 -0.3
stress Vanillin 5 mM +0.4 +0.2 -0.5 -3.3 -0.6
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.4 +0.4 +0.7 -3.6 -0.7
phage Phage_N4_cage_4_mouse_1_Cecum_40 hr +0.1 +0.2 -1.0 -1.7 -1.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.0 +0.2 -0.8 -1.8 -1.0
phage T7_phage 0.00015625 MOI TC4 -0.4 +0.1 -0.2 -2.8 -0.1
control PBS_cage_2_mouse_1_Cecum_40 hr +0.1 +0.3 -1.5 -0.9 -1.2
stress Chlorite 1.25 mM -0.2 +0.6 -1.5 -0.9 -1.1
phage N4_phage 0.009375 MOI TC2 +0.5 -0.5 +0.0 -2.0 -0.8
phage Phage_N4_cage_3_mice _Feces_40 hr +0.2 +0.4 -0.7 -1.9 -0.7
stress Ciprofloxacin Hydrochloride 0.0024 mM +0.4 -0.2 +0.2 -2.7 -0.2
stress Trimethoprim 0.005 mM -0.2 +1.0 -0.9 -1.3 -1.0
control PBS_cage_2_mice _Feces_40 hr +0.2 +0.7 -1.0 -0.7 -1.0
stress Benzalkonium Chloride 0.004 mg/ml +0.4 +0.5 -0.7 +0.9 -1.3
phage Ste41 -0.5 +0.4 -1.0 +1.7 -0.2
stress Aluminum chloride 5 mM +0.1 +0.4 +0.9 +1.0 +1.3
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