Fitness for 5 genes in Escherichia coli BW25113

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500 ntfadD and yeaY are separated by 204 nucleotidesyeaY and yeaZ are separated by 39 nucleotidesyeaZ and yoaA are separated by 57 nucleotidesyoaA and b1809 are separated by 131 nucleotides b1805: fadD - acyl-CoA synthase (NCBI), at 1,886,085 to 1,887,770 fadD b1806: yeaY - predicted lipoprotein (NCBI), at 1,887,975 to 1,888,556 yeaY b1807: yeaZ - predicted peptidase (NCBI), at 1,888,596 to 1,889,291 yeaZ b1808: yoaA - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 1,889,349 to 1,891,259 yoaA b1809: b1809 - orf, hypothetical protein (VIMSS), at 1,891,391 to 1,891,735 b1809
Group Condition b1805 b1806 b1807 b1808 b1809
fadD yeaY yeaZ yoaA b1809
carbon source Glycolic Acid (C) -2.1 -0.0 N.D. +0.0 +0.0
phage N4_phage 0.009375 MOI TC2 -1.4 +0.5 N.D. -0.5 -0.6
phage RB68 -0.1 -0.5 N.D. -0.7 -0.6
phage N4_phage 0.0009375 MOI TC2 -0.0 -1.1 N.D. -0.3 -0.5
carbon source Glycolic Acid (C) -1.7 -0.0 N.D. -0.2 +0.1
stress LB Aerobic with Ramoplanin 0.000125 mM -1.4 -0.5 N.D. +0.5 -0.4
stress Chloride 750 mM +0.1 -0.9 N.D. -0.6 -0.3
stress Cisplatin 0.1 mg/ml +0.6 -0.7 N.D. -0.2 -0.8
stress Irgasan 0.0024 mM -1.1 +0.3 N.D. -0.3 +0.2
stress LB Anaerobic with Levofloxacin 0.0000000625 mM +0.2 -0.3 N.D. -1.0 +0.4
carbon source Dodecanol (C) -1.2 +0.2 N.D. +0.1 +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.3 +0.1 N.D. -0.8 +0.6
stress LB Aerobic with Ramoplanin 0.000125 mM -1.2 +0.7 N.D. +0.4 +0.2
stress 2,4-Dinitrophenol 2 mM +1.1 -0.3 N.D. -0.5 -0.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +1.2 -1.0 N.D. +0.0 +0.0
stress Spectinomycin 0.0125 mg/ml +0.5 +0.4 N.D. +0.2 -0.7
phage N4_phage 0.09375 MOI TC1 -0.2 +1.1 N.D. +0.2 -0.3
phage T7_phage 0.00015625 MOI TC4 +1.8 -0.8 N.D. +0.2 -0.2
stress LB Anaerobic with Nalidixic 0.0000078125 mM +0.3 -0.5 N.D. +1.2 +0.2
stress Nalidixic 0.006 mg/ml +0.3 +0.1 N.D. +1.4 -0.0
stress Nalidixic 0.06 mM +0.4 +0.2 N.D. +1.0 +0.3
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +1.4 +0.2 N.D. +0.2 +0.1
motility outer cut, LB soft agar motility assay +0.7 +0.3 N.D. +0.7 +0.2
motility inner cut, LB soft agar motility assay +0.6 +1.1 N.D. +0.3 -0.1
stress LB Anaerobic with Nalidixic 0.0000078125 mM +0.3 +0.3 N.D. +1.1 +0.2
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +1.3 +0.4 N.D. +0.2 +0.3
stress Bacitracin 1 mg/ml +1.5 +0.4 N.D. +0.2 +0.2
stress octanol 0.08 vol% +1.1 +0.6 N.D. +0.8 -0.2
stress Benzalkonium Chloride 0.004 mg/ml +1.4 +0.8 N.D. +0.5 +0.4
stress LB Aerobic with Irgasan 0.0000003125 mM +7.7 +4.7 N.D. -0.1 -0.3
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