Fitness for 5 genes in Escherichia coli BW25113

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500 ntyeaX and rnd are separated by 53 nucleotidesrnd and fadD are separated by 69 nucleotidesfadD and yeaY are separated by 204 nucleotidesyeaY and yeaZ are separated by 39 nucleotides b1803: yeaX - predicted oxidoreductase (NCBI), at 1,883,869 to 1,884,834 yeaX b1804: rnd - ribonuclease D (NCBI), at 1,884,888 to 1,886,015 rnd b1805: fadD - acyl-CoA synthase (NCBI), at 1,886,085 to 1,887,770 fadD b1806: yeaY - predicted lipoprotein (NCBI), at 1,887,975 to 1,888,556 yeaY b1807: yeaZ - predicted peptidase (NCBI), at 1,888,596 to 1,889,291 yeaZ
Group Condition b1803 b1804 b1805 b1806 b1807
yeaX rnd fadD yeaY yeaZ
stress LB Aerobic with Ramoplanin 0.000125 mM +0.1 -2.0 -1.4 -0.5 N.D.
predation 6 hr rep2_HD100_10fold_dilution_grewON +0.0 -2.4 -0.7 +0.5 N.D.
phage RB68 +0.2 -1.9 -0.1 -0.5 N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.7 -1.6 -0.2 +0.4 N.D.
motility outer cut, LB soft agar motility assay -0.1 -2.4 +0.3 +0.1 N.D.
stress LB Aerobic with Ramoplanin 0.000125 mM -0.1 -1.4 -1.2 +0.7 N.D.
phage Lambda_ci1857 +0.1 -2.1 +0.3 -0.3 N.D.
motility outer cut, LB soft agar motility assay -0.2 -2.7 +0.7 +0.3 N.D.
stress 3-methyl-1-butanol 1.25 vol% +0.6 -1.3 -0.5 -0.6 N.D.
motility inner cut, LB soft agar motility assay +0.6 -2.0 +0.3 -0.2 N.D.
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.7 -1.2 +0.4 -0.9 N.D.
stress octanol 0.08 vol% -0.7 -1.8 +1.1 +0.6 N.D.
carbon source Glycolic Acid (C) -0.1 +1.8 -2.1 -0.0 N.D.
carbon source carbon source D-Glucose 0.25mM -0.3 -1.4 +0.6 +0.8 N.D.
stress 2-Furfuraldehyde 0.125 vol% +0.1 -1.6 +0.2 +1.0 N.D.
motility inner cut, LB soft agar motility assay -0.4 -1.3 +0.6 +1.1 N.D.
carbon source Glycolic Acid (C) -0.1 +1.9 -1.7 -0.0 N.D.
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.2 -1.2 +1.3 +0.4 N.D.
stress Bacitracin 1 mg/ml +0.1 -1.2 +1.5 +0.4 N.D.
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.6 -0.8 +1.4 +0.2 N.D.
stress Benzalkonium Chloride 0.004 mg/ml +0.4 -1.2 +1.4 +0.8 N.D.
phage T7_phage 0.00015625 MOI TC4 +0.5 +0.2 +1.8 -0.8 N.D.
carbon source L-Malic (C) +0.1 +1.9 -0.4 +0.4 N.D.
stress Trimethoprim 0.00125 mM +0.1 +2.8 -0.4 +0.1 N.D.
stress Trimethoprim 0.0025 mM -0.2 +3.1 -0.2 -0.0 N.D.
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.1 +2.4 -0.0 +0.3 N.D.
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.3 +2.6 -0.1 +0.2 N.D.
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +1.1 +2.4 +1.2 -1.0 N.D.
stress Trimethoprim 0.005 mM +0.5 +3.5 -0.1 -0.2 N.D.
stress LB Aerobic with Irgasan 0.0000003125 mM -0.0 -0.4 +7.7 +4.7 N.D.
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