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Fitness for 5 genes in
Escherichia coli BW25113
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
yeaV and yeaW are separated by 31 nucleotides
yeaW and yeaX are separated by 55 nucleotides
yeaX and rnd are separated by 53 nucleotides
rnd and fadD are separated by 69 nucleotides
b1801: yeaV - predicted transporter (NCBI), at 1,881,212 to 1,882,657
yeaV
b1802: yeaW - predicted 2Fe-2S cluster-containing protein (NCBI), at 1,882,689 to 1,883,813
yeaW
b1803: yeaX - predicted oxidoreductase (NCBI), at 1,883,869 to 1,884,834
yeaX
b1804: rnd - ribonuclease D (NCBI), at 1,884,888 to 1,886,015
rnd
b1805: fadD - acyl-CoA synthase (NCBI), at 1,886,085 to 1,887,770
fadD
Group
Condition
b1801
b1802
b1803
b1804
b1805
yeaV
yeaW
yeaX
rnd
fadD
motility
outer cut, LB soft agar motility assay
-0.2
-1.3
-0.2
-2.7
+0.7
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.6
+0.6
+0.1
-2.0
-1.4
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.5
-0.1
-0.1
-1.4
-1.2
predation
6 hr rep2_HD100_10fold_dilution_grewON
-0.2
+0.1
+0.0
-2.4
-0.7
phage
Lambda_ci1857
-0.2
-0.0
+0.1
-2.1
+0.3
motility
outer cut, LB soft agar motility assay
-0.2
+0.6
-0.1
-2.4
+0.3
stress
octanol 0.08 vol%
+0.0
-0.3
-0.7
-1.8
+1.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.3
+0.7
-0.7
-1.6
-0.2
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-0.5
-0.4
+0.7
-1.2
+0.4
motility
outer cut, LB soft agar motility assay
+0.2
+0.2
-0.3
-1.7
+0.6
motility
inner cut, LB soft agar motility assay
+0.6
+0.2
-0.4
-1.3
+0.6
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
-0.1
-0.1
-0.2
-1.2
+1.3
motility
inner cut, LB soft agar motility assay
+0.7
+0.3
+0.6
-2.0
+0.3
carbon source
Glycolic Acid (C)
-0.1
+0.3
-0.1
+1.8
-2.1
stress
Bacitracin 1 mg/ml
-0.5
+0.2
+0.1
-1.2
+1.5
carbon source
Glycolic Acid (C)
-0.0
+0.2
-0.1
+1.9
-1.7
stress
Benzalkonium Chloride 0.004 mg/ml
+0.0
+0.2
+0.4
-1.2
+1.4
stress
Cisplatin 0.1 mg/ml
-0.5
-0.6
+0.1
+1.3
+0.6
phage
T7_phage 0.00015625 MOI TC4
-0.7
-0.4
+0.5
+0.2
+1.8
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.4
-0.1
+0.6
-0.8
+1.4
stress
Trimethoprim 0.005 mM
-0.1
-2.0
+0.5
+3.5
-0.1
gfp_control
GFP_control
+0.4
+1.0
+1.4
-0.4
+0.1
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
+0.2
-0.2
+0.1
+2.4
-0.0
stress
Spectinomycin 0.0125 mg/ml
+0.1
+0.6
+0.2
+1.5
+0.5
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
+0.4
+1.6
+0.0
+1.4
-0.4
stress
Trimethoprim 0.00125 mM
+0.2
+0.3
+0.1
+2.8
-0.4
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
+0.2
+0.2
+0.3
+2.6
-0.1
stress
Trimethoprim 0.0025 mM
+0.2
+0.6
-0.2
+3.1
-0.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.3
-1.3
+1.1
+2.4
+1.2
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.2
+0.2
-0.0
-0.4
+7.7
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