Fitness for 5 genes in Escherichia coli BW25113

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500 ntyeaV and yeaW are separated by 31 nucleotidesyeaW and yeaX are separated by 55 nucleotidesyeaX and rnd are separated by 53 nucleotidesrnd and fadD are separated by 69 nucleotides b1801: yeaV - predicted transporter (NCBI), at 1,881,212 to 1,882,657 yeaV b1802: yeaW - predicted 2Fe-2S cluster-containing protein (NCBI), at 1,882,689 to 1,883,813 yeaW b1803: yeaX - predicted oxidoreductase (NCBI), at 1,883,869 to 1,884,834 yeaX b1804: rnd - ribonuclease D (NCBI), at 1,884,888 to 1,886,015 rnd b1805: fadD - acyl-CoA synthase (NCBI), at 1,886,085 to 1,887,770 fadD
Group Condition b1801 b1802 b1803 b1804 b1805
yeaV yeaW yeaX rnd fadD
motility outer cut, LB soft agar motility assay -0.2 -1.3 -0.2 -2.7 +0.7
stress LB Aerobic with Ramoplanin 0.000125 mM -0.6 +0.6 +0.1 -2.0 -1.4
stress LB Aerobic with Ramoplanin 0.000125 mM -0.5 -0.1 -0.1 -1.4 -1.2
predation 6 hr rep2_HD100_10fold_dilution_grewON -0.2 +0.1 +0.0 -2.4 -0.7
phage Lambda_ci1857 -0.2 -0.0 +0.1 -2.1 +0.3
motility outer cut, LB soft agar motility assay -0.2 +0.6 -0.1 -2.4 +0.3
stress octanol 0.08 vol% +0.0 -0.3 -0.7 -1.8 +1.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.3 +0.7 -0.7 -1.6 -0.2
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.5 -0.4 +0.7 -1.2 +0.4
motility outer cut, LB soft agar motility assay +0.2 +0.2 -0.3 -1.7 +0.6
motility inner cut, LB soft agar motility assay +0.6 +0.2 -0.4 -1.3 +0.6
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM -0.1 -0.1 -0.2 -1.2 +1.3
motility inner cut, LB soft agar motility assay +0.7 +0.3 +0.6 -2.0 +0.3
carbon source Glycolic Acid (C) -0.1 +0.3 -0.1 +1.8 -2.1
stress Bacitracin 1 mg/ml -0.5 +0.2 +0.1 -1.2 +1.5
carbon source Glycolic Acid (C) -0.0 +0.2 -0.1 +1.9 -1.7
stress Benzalkonium Chloride 0.004 mg/ml +0.0 +0.2 +0.4 -1.2 +1.4
stress Cisplatin 0.1 mg/ml -0.5 -0.6 +0.1 +1.3 +0.6
phage T7_phage 0.00015625 MOI TC4 -0.7 -0.4 +0.5 +0.2 +1.8
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.4 -0.1 +0.6 -0.8 +1.4
stress Trimethoprim 0.005 mM -0.1 -2.0 +0.5 +3.5 -0.1
gfp_control GFP_control +0.4 +1.0 +1.4 -0.4 +0.1
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.2 -0.2 +0.1 +2.4 -0.0
stress Spectinomycin 0.0125 mg/ml +0.1 +0.6 +0.2 +1.5 +0.5
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.4 +1.6 +0.0 +1.4 -0.4
stress Trimethoprim 0.00125 mM +0.2 +0.3 +0.1 +2.8 -0.4
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM +0.2 +0.2 +0.3 +2.6 -0.1
stress Trimethoprim 0.0025 mM +0.2 +0.6 -0.2 +3.1 -0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.3 -1.3 +1.1 +2.4 +1.2
stress LB Aerobic with Irgasan 0.0000003125 mM +0.2 +0.2 -0.0 -0.4 +7.7
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