Fitness for 5 genes in Escherichia coli BW25113

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500 ntastA and astC overlap by 4 nucleotidesastC and xthA are separated by 445 nucleotidesxthA and ydjX are separated by 166 nucleotidesydjX and ydjY are separated by 4 nucleotides b1747: astA - arginine succinyltransferase (NCBI), at 1,827,755 to 1,828,789 astA b1748: astC - succinylornithine transaminase, PLP-dependent (NCBI), at 1,828,786 to 1,830,006 astC b1749: xthA - exonuclease III (NCBI), at 1,830,452 to 1,831,258 xthA b1750: ydjX - orf, hypothetical protein (VIMSS), at 1,831,425 to 1,832,135 ydjX b1751: ydjY - orf, hypothetical protein (VIMSS), at 1,832,140 to 1,832,817 ydjY
Group Condition b1747 b1748 b1749 b1750 b1751
astA astC xthA ydjX ydjY
nitrogen source L-Arginine (N) -3.9 -4.1 +0.3 -0.3 +0.1
nitrogen source L-Arginine (N) -3.4 -3.7 -0.2 -0.3 -0.1
fishing LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH22 VcDART vector -1.8 -2.1 +0.2 -0.0 +0.0
dart Outgrowth from conjugation with pRH22 in LB+Gent50 -1.3 -1.6 -0.3 -0.2 +0.0
dart Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100 -1.2 -1.7 +0.4 -0.1 -0.2
dart Outgrowth from conjugation with pRH22 in LB+Gent50 -1.2 -1.7 +0.1 -0.2 +0.2
phage Ste41 +0.5 +0.1 -2.7 -0.5 -0.1
dart Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100 -1.2 -1.6 +0.0 -0.0 +0.1
dart Outgrowth from conjugation with pRH23 in LB+Gent50 -1.4 -1.2 +0.2 -0.1 -0.1
stress benzenesulfonyl fluoride -0.2 -0.2 -2.3 -0.1 +0.1
dart Outgrowth from conjugation with pRH23 in LB+Gent50 -1.3 -1.5 +0.1 -0.0 +0.2
dart Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100 -1.1 -1.5 +0.3 +0.0 -0.1
stress Trimethoprim 0.005 mM -0.5 -0.3 -1.3 -0.3 +0.2
dart Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100 -1.2 -1.3 +0.4 -0.0 +0.0
phage Δdbn_10 -0.4 -0.8 -0.4 +0.2 -0.6
fishing LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH23 VcDART vector -0.9 -1.3 +0.3 +0.1 -0.2
phage RB68 +0.5 -0.7 -1.1 -0.6 +0.1
stress methylglyoxal 0.008 vol% -0.9 -0.7 -0.7 +0.2 +0.3
carbon source carbon source D-Mannose 1mM +0.1 +0.5 -0.6 +0.0 -1.1
phage Ste41 +0.7 -0.5 -0.4 +0.1 -0.8
carbon source carbon source D-Glucose 0.25mM -1.2 -0.2 +0.5 -0.5 +0.6
stress Cisplatin 0.1 mg/ml +0.4 -1.0 -1.5 +0.9 +0.5
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM +0.1 -0.6 -0.8 +0.2 +0.7
carbon source carbon source D-Mannose 1mM +0.3 -0.4 +0.6 +0.2 -0.8
phage T7_phage 0.0015625 MOI TC1 +0.3 -0.4 -0.7 +0.7 +0.5
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -0.5 +0.5 +1.2 -0.4 -0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.3 +0.2 +0.3 -0.8 -0.2
phage T7_phage 0.00015625 MOI TC3 -0.2 -0.2 +1.7 +0.4 +0.5
motility outer cut, LB soft agar motility assay +0.8 +0.6 +1.1 -0.2 +0.3
phage T7_phage 0.0015625 MOI TC2 -0.8 -0.0 +2.5 +0.3 +1.2
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