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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
astA and astC overlap by 4 nucleotides
astC and xthA are separated by 445 nucleotides
xthA and ydjX are separated by 166 nucleotides
ydjX and ydjY are separated by 4 nucleotides
b1747: astA - arginine succinyltransferase (NCBI), at 1,827,755 to 1,828,789
astA
b1748: astC - succinylornithine transaminase, PLP-dependent (NCBI), at 1,828,786 to 1,830,006
astC
b1749: xthA - exonuclease III (NCBI), at 1,830,452 to 1,831,258
xthA
b1750: ydjX - orf, hypothetical protein (VIMSS), at 1,831,425 to 1,832,135
ydjX
b1751: ydjY - orf, hypothetical protein (VIMSS), at 1,832,140 to 1,832,817
ydjY
Group
Condition
b1747
b1748
b1749
b1750
b1751
astA
astC
xthA
ydjX
ydjY
nitrogen source
L-Arginine (N)
-3.9
-4.1
+0.3
-0.3
+0.1
nitrogen source
L-Arginine (N)
-3.4
-3.7
-0.2
-0.3
-0.1
fishing
LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH22 VcDART vector
-1.8
-2.1
+0.2
-0.0
+0.0
dart
Outgrowth from conjugation with pRH22 in LB+Gent50
-1.3
-1.6
-0.3
-0.2
+0.0
dart
Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100
-1.2
-1.7
+0.4
-0.1
-0.2
dart
Outgrowth from conjugation with pRH22 in LB+Gent50
-1.2
-1.7
+0.1
-0.2
+0.2
phage
Ste41
+0.5
+0.1
-2.7
-0.5
-0.1
dart
Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100
-1.2
-1.6
+0.0
-0.0
+0.1
dart
Outgrowth from conjugation with pRH23 in LB+Gent50
-1.4
-1.2
+0.2
-0.1
-0.1
stress
benzenesulfonyl fluoride
-0.2
-0.2
-2.3
-0.1
+0.1
dart
Outgrowth from conjugation with pRH23 in LB+Gent50
-1.3
-1.5
+0.1
-0.0
+0.2
dart
Outgrowth from conjugation with pRH22 in LB+Gent50+Kan100
-1.1
-1.5
+0.3
+0.0
-0.1
stress
Trimethoprim 0.005 mM
-0.5
-0.3
-1.3
-0.3
+0.2
dart
Outgrowth from conjugation with pRH23 in LB+Gent50+Kan100
-1.2
-1.3
+0.4
-0.0
+0.0
phage
Δdbn_10
-0.4
-0.8
-0.4
+0.2
-0.6
fishing
LB+Gent outgrowth of mutant library after 1 day conjugation with donor carrying pRH23 VcDART vector
-0.9
-1.3
+0.3
+0.1
-0.2
phage
RB68
+0.5
-0.7
-1.1
-0.6
+0.1
stress
methylglyoxal 0.008 vol%
-0.9
-0.7
-0.7
+0.2
+0.3
carbon source
carbon source D-Mannose 1mM
+0.1
+0.5
-0.6
+0.0
-1.1
phage
Ste41
+0.7
-0.5
-0.4
+0.1
-0.8
carbon source
carbon source D-Glucose 0.25mM
-1.2
-0.2
+0.5
-0.5
+0.6
stress
Cisplatin 0.1 mg/ml
+0.4
-1.0
-1.5
+0.9
+0.5
stress
LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM
+0.1
-0.6
-0.8
+0.2
+0.7
carbon source
carbon source D-Mannose 1mM
+0.3
-0.4
+0.6
+0.2
-0.8
phage
T7_phage 0.0015625 MOI TC1
+0.3
-0.4
-0.7
+0.7
+0.5
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
-0.5
+0.5
+1.2
-0.4
-0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+1.3
+0.2
+0.3
-0.8
-0.2
phage
T7_phage 0.00015625 MOI TC3
-0.2
-0.2
+1.7
+0.4
+0.5
motility
outer cut, LB soft agar motility assay
+0.8
+0.6
+1.1
-0.2
+0.3
phage
T7_phage 0.0015625 MOI TC2
-0.8
-0.0
+2.5
+0.3
+1.2
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