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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
pfkB and ydiZ are separated by 100 nucleotides
ydiZ and yniA are separated by 105 nucleotides
yniA and yniB are separated by 40 nucleotides
yniB and yniC are separated by 146 nucleotides
b1723: pfkB - 6-phosphofructokinase II; suppressor of pfkA (VIMSS), at 1,804,394 to 1,805,323
pfkB
b1724: ydiZ - hypothetical protein (NCBI), at 1,805,424 to 1,805,714
ydiZ
b1725: yniA - predicted phosphotransferase/kinase (NCBI), at 1,805,820 to 1,806,680
yniA
b1726: yniB - predicted inner membrane protein (NCBI), at 1,806,721 to 1,807,257
yniB
b1727: yniC - predicted hydrolase (NCBI), at 1,807,404 to 1,808,072
yniC
Group
Condition
b1723
b1724
b1725
b1726
b1727
pfkB
ydiZ
yniA
yniB
yniC
nophagecontrol
R1_N4_T1_phage0
-0.4
-1.7
-0.2
-0.5
-2.8
carbon source
D-Glucosamine Hydrochloride (C)
-0.2
-1.5
+0.3
+0.2
-3.4
carbon source
carbon source D-Mannose 0.5mM
-0.3
-0.7
+0.0
-3.6
+0.1
mzaabcde
mzaABCDE
-0.3
-0.6
-0.2
-3.4
+0.1
mzaabcde
mzaABCDE
-0.1
-1.3
-0.6
-2.0
-0.2
phage
Δdbn_10
+0.4
+0.5
+0.2
-0.3
-4.5
stress
LB Anaerobic with Ampicillin 0.00000195313 mM
+0.2
-1.0
-0.5
-1.7
-0.6
stress
Chlorite 1.25 mM
+0.0
-0.3
-0.7
-0.1
-2.3
stress
methylglyoxal 0.008 vol%
+0.2
-1.4
-0.3
-1.7
-0.2
predation
6 hr rep2_HD100_10fold_dilution_grewON
+0.4
-0.5
+0.0
-2.6
-0.6
gfp_control
GFP_control
+0.1
-2.1
+0.1
-1.2
-0.1
carbon source
carbon source D-Mannose 0.5mM
-0.3
-0.7
-0.3
-2.0
+0.2
carbon source
D-Glucosamine Hydrochloride (C)
-0.1
-1.0
+0.2
+0.3
-2.1
micoeukaryotes
Tetrahymena sp., Mixed culture-1
-0.0
+0.4
-0.5
-0.2
-2.3
stress
Benzalkonium Chloride 0.004 mg/ml
-0.5
+0.5
-0.1
-1.7
-0.7
balo
Mixed culture, Halobacteriovorax-1
-0.3
+1.1
+0.1
-1.8
-1.0
phage
Ste41
-0.2
+1.0
-0.7
-0.1
-1.7
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.5
-0.8
+0.7
-1.7
+0.4
control
PBS_cage_1_mice _Feces_16 hr
-0.7
-1.5
+0.6
+0.3
+0.6
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.5
+1.3
-1.1
-0.3
-0.6
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.2
-0.1
+0.0
+1.6
-1.8
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.2
-0.0
+0.3
+2.0
-1.7
stress
Trimethoprim 0.005 mM
-0.4
+0.1
+0.8
+1.3
-1.6
carbon source
acetate (C)
-0.2
-0.8
+2.2
+0.2
-0.4
control
PBS_cage_2_mouse_2_Cecum_40 hr
+0.3
+0.9
-0.2
-1.0
+1.1
M9 Acetate 0.5% aerobic
-0.0
+0.0
+2.3
-1.1
-0.0
M9 Acetate 0.5% aerobic
-0.1
+0.1
+2.3
-0.8
+0.2
carbon source
acetate (C)
-0.3
-0.5
+2.4
+0.2
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+0.2
-0.3
-0.1
+3.2
-0.7
stress
Ciprofloxacin Hydrochloride 0.0024 mM
+0.4
+0.8
+0.1
+0.8
+1.3
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