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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
rprA and b1689 are separated by 137 nucleotides
b1689 and ydiM are separated by 99 nucleotides
ydiM and ydiN are separated by 226 nucleotides
ydiN and ydiB are separated by 11 nucleotides
b4431: rprA - Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq), at 1,768,396 to 1,768,501
rprA
b1689: b1689 - orf, hypothetical protein (VIMSS), at 1,768,639 to 1,768,995
b1689
b1690: ydiM - predicted transporter (NCBI), at 1,769,095 to 1,770,309
ydiM
b1691: ydiN - predicted transporter (RefSeq), at 1,770,536 to 1,771,801
ydiN
b1692: ydiB - quinate/shikimate 5-dehydrogenase, NAD(P)-binding (NCBI), at 1,771,813 to 1,772,679
ydiB
Group
Condition
b4431
b1689
b1690
b1691
b1692
rprA
b1689
ydiM
ydiN
ydiB
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
-6.2
-0.4
-0.6
-1.7
-1.1
carbon source
carbon source D-Mannose 0.5mM
-5.3
-1.5
-0.4
-0.3
-2.3
M9 Acetate 0.5% aerobic
-0.9
-0.8
-1.9
-1.3
-0.5
M9 Acetate 0.5% aerobic
-0.4
-0.7
-1.9
-1.4
-0.9
M9 Glucose 2% anaerobic
-0.7
-0.7
-1.8
-1.2
-0.7
M9 Glucose 2%, 20 mM NO3- anaerobic
-0.8
-0.6
-2.0
-1.1
-0.6
M9 Glucose 2% aerobic
-0.6
-0.6
-1.9
-1.2
-0.7
carbon source
carbon source D-Glucose 20mM
-2.8
+0.2
-0.3
-0.5
-1.3
M9 Glucose 2% aerobic
-0.4
-0.5
-2.0
-1.2
-0.6
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.4
-1.7
-0.4
-0.9
-1.0
carbon source
carbon source D-Mannose 0.5mM
-2.8
-0.4
-0.7
+0.0
-0.6
stress
LB Anaerobic with Azlocillin 0.00003125 mM
-3.1
-0.2
-0.5
-0.3
-0.4
phage
Phage_N4_cage_3_mouse_1_Cecum_40 hr
-0.7
-0.3
-1.5
-1.1
-0.8
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
-0.4
-1.0
-0.4
-1.8
-0.4
motility
outer cut, LB soft agar motility assay
-1.0
-0.9
-0.8
-0.5
-0.9
control
PBS_cage_1_mouse_2_Cecum_40 hr
+0.1
-2.2
+0.4
-1.8
-0.5
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-3.4
-0.6
-0.4
+0.3
+0.2
balo
Mixed culture, Halobacteriovorax-1
-1.8
-0.5
-0.5
-0.7
+0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-3.0
-1.1
+1.1
+0.2
+0.1
control
PBS_cage_1_mice _Feces_40 hr
+0.5
-1.5
+1.2
-1.4
+0.3
control
PBS_cage_1_mice _Feces_16 hr
+0.2
-2.1
+1.5
-1.0
+0.5
control
PBS_cage_1_mouse_1_Cecum_40 hr
+0.7
-1.9
+0.3
-1.2
+1.2
control
PBS_cage_1_mice _Feces_22 hr
+0.5
-0.9
+1.2
-1.3
+0.5
phage
Ste41
-0.8
-0.7
+1.3
+0.7
+0.6
phage
N4_phage 0.09375 MOI TC1
-0.9
-0.5
+1.5
+0.9
+0.6
phage
T7_phage 0.0015625 MOI TC2
-0.9
+1.0
+1.3
+0.6
+0.7
phage
Ste41
+1.0
+0.1
+1.3
+0.9
+0.8
phage
N4_phage 0.009375 MOI TC2
+0.6
+0.1
+2.3
+1.8
+0.5
phage
Papa dilution 10-1 cacl2 maltose
+0.2
+1.2
+2.2
+1.0
+0.8
stress
Trimethoprim 0.005 mM
+0.0
+0.2
+2.7
+2.1
+0.8
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