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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ydiI and ydiJ overlap by 4 nucleotides
ydiJ and ydiK are separated by 388 nucleotides
ydiK and rprA are separated by 185 nucleotides
rprA and b1689 are separated by 137 nucleotides
b1686: ydiI - hypothetical protein (NCBI), at 1,763,246 to 1,763,656
ydiI
b1687: ydiJ - predicted FAD-linked oxidoreductase (NCBI), at 1,763,653 to 1,766,709
ydiJ
b1688: ydiK - predicted inner membrane protein (NCBI), at 1,767,098 to 1,768,210
ydiK
b4431: rprA - Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq), at 1,768,396 to 1,768,501
rprA
b1689: b1689 - orf, hypothetical protein (VIMSS), at 1,768,639 to 1,768,995
b1689
Group
Condition
b1686
b1687
b1688
b4431
b1689
ydiI
ydiJ
ydiK
rprA
b1689
carbon source
carbon source D-Mannose 5mM
-6.1
-0.8
-1.6
+0.1
+0.7
carbon source
carbon source D-Mannose 0.5mM
+0.1
-0.1
-0.4
-5.3
-1.5
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-2.2
-0.2
-0.2
-3.4
-0.6
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
+0.4
+0.3
-0.2
-6.2
-0.4
stress
LB Anaerobic with Levofloxacin 0.00000003125 mM
-2.5
-0.0
-0.1
-1.2
-0.5
no stress control anaerobic
M9 no stress control; Anaerobic 24 hr
-1.2
-2.1
-0.3
-0.5
-0.1
stress
LB Anaerobic with Ampicillin 0.00000195313 mM
-3.2
+0.3
-0.0
-0.8
-0.3
control
PBS_cage_1_mice _Feces_16 hr
-1.1
-0.3
-0.6
+0.2
-2.1
control
PBS_cage_1_mice _Feces_40 hr
-1.8
-0.5
-0.5
+0.5
-1.5
carbon source
carbon source D-Mannose 0.5mM
+0.1
-0.5
-0.1
-2.8
-0.4
no stress control anaerobic
M9 no stress control; Anaerobic 24 hr
-1.5
-1.6
-0.2
-0.3
+0.0
stress
LB Anaerobic with Sulfamonomethoxine 0.00003125 mM
-2.0
+0.1
+0.0
-1.3
-0.3
no stress control anaerobic
M9 no stress control; Anaerobic 48 hr
-1.2
-2.0
+0.0
+0.1
-0.4
stress
LB Anaerobic with Azlocillin 0.00003125 mM
-0.1
-0.0
+0.0
-3.1
-0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.8
+0.4
+0.0
-3.0
-1.1
no stress control anaerobic
M9 no stress control; Anaerobic 48 hr
-1.1
-1.9
-0.0
+0.4
+0.1
carbon source
carbon source D-Glucose 20mM
+0.3
-0.2
-0.0
-2.8
+0.2
no stress control anaerobic
M9 no stress control; Anaerobic 24 hr
-1.2
-1.7
+0.1
+0.5
+0.1
balo
Mixed culture, Halobacteriovorax-1
+0.5
-0.5
+0.2
-1.8
-0.5
phage
N4_phage 0.0009375 MOI TC3
-1.9
+0.4
-0.0
-1.1
+0.5
phage
TP4
-2.2
+0.2
-0.1
-0.7
+1.0
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.3
+0.0
+0.5
+0.7
-1.9
phage
N4_phage 0.0009375 MOI TC2
+0.7
+0.3
+0.3
-1.4
-0.8
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+1.1
+0.5
-0.2
-1.3
-0.9
stress
Cisplatin 0.1 mg/ml
+1.4
+0.2
+0.2
-1.3
-0.3
carbon source
carbon source D-Glucose 0.25mM
+2.6
-0.4
+0.1
-1.1
-0.9
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+1.1
+0.5
-0.6
-1.0
+0.4
motility
inner cut, LB soft agar motility assay
+2.2
+0.1
-0.3
-0.8
-0.1
predation
6 hr rep2_HD100_10fold_dilution_grewON
+1.7
-0.2
-0.3
+1.3
-0.8
carbon source
carbon source D-Glucose 5mM
+1.1
-0.2
-0.6
+1.0
+1.2
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