Fitness for 5 genes in Escherichia coli BW25113

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500 ntydiI and ydiJ overlap by 4 nucleotidesydiJ and ydiK are separated by 388 nucleotidesydiK and rprA are separated by 185 nucleotidesrprA and b1689 are separated by 137 nucleotides b1686: ydiI - hypothetical protein (NCBI), at 1,763,246 to 1,763,656 ydiI b1687: ydiJ - predicted FAD-linked oxidoreductase (NCBI), at 1,763,653 to 1,766,709 ydiJ b1688: ydiK - predicted inner membrane protein (NCBI), at 1,767,098 to 1,768,210 ydiK b4431: rprA - Positive regulatory sRNA for RpoS translation; non-essential gene (RefSeq), at 1,768,396 to 1,768,501 rprA b1689: b1689 - orf, hypothetical protein (VIMSS), at 1,768,639 to 1,768,995 b1689
Group Condition b1686 b1687 b1688 b4431 b1689
ydiI ydiJ ydiK rprA b1689
carbon source carbon source D-Mannose 5mM -6.1 -0.8 -1.6 +0.1 +0.7
carbon source carbon source D-Mannose 0.5mM +0.1 -0.1 -0.4 -5.3 -1.5
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -2.2 -0.2 -0.2 -3.4 -0.6
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr +0.4 +0.3 -0.2 -6.2 -0.4
stress LB Anaerobic with Levofloxacin 0.00000003125 mM -2.5 -0.0 -0.1 -1.2 -0.5
no stress control anaerobic M9 no stress control; Anaerobic 24 hr -1.2 -2.1 -0.3 -0.5 -0.1
stress LB Anaerobic with Ampicillin 0.00000195313 mM -3.2 +0.3 -0.0 -0.8 -0.3
control PBS_cage_1_mice _Feces_16 hr -1.1 -0.3 -0.6 +0.2 -2.1
control PBS_cage_1_mice _Feces_40 hr -1.8 -0.5 -0.5 +0.5 -1.5
carbon source carbon source D-Mannose 0.5mM +0.1 -0.5 -0.1 -2.8 -0.4
no stress control anaerobic M9 no stress control; Anaerobic 24 hr -1.5 -1.6 -0.2 -0.3 +0.0
stress LB Anaerobic with Sulfamonomethoxine 0.00003125 mM -2.0 +0.1 +0.0 -1.3 -0.3
no stress control anaerobic M9 no stress control; Anaerobic 48 hr -1.2 -2.0 +0.0 +0.1 -0.4
stress LB Anaerobic with Azlocillin 0.00003125 mM -0.1 -0.0 +0.0 -3.1 -0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.8 +0.4 +0.0 -3.0 -1.1
no stress control anaerobic M9 no stress control; Anaerobic 48 hr -1.1 -1.9 -0.0 +0.4 +0.1
carbon source carbon source D-Glucose 20mM +0.3 -0.2 -0.0 -2.8 +0.2
no stress control anaerobic M9 no stress control; Anaerobic 24 hr -1.2 -1.7 +0.1 +0.5 +0.1
balo Mixed culture, Halobacteriovorax-1 +0.5 -0.5 +0.2 -1.8 -0.5
phage N4_phage 0.0009375 MOI TC3 -1.9 +0.4 -0.0 -1.1 +0.5
phage TP4 -2.2 +0.2 -0.1 -0.7 +1.0
control PBS_cage_1_mouse_1_Cecum_40 hr -0.3 +0.0 +0.5 +0.7 -1.9
phage N4_phage 0.0009375 MOI TC2 +0.7 +0.3 +0.3 -1.4 -0.8
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.1 +0.5 -0.2 -1.3 -0.9
stress Cisplatin 0.1 mg/ml +1.4 +0.2 +0.2 -1.3 -0.3
carbon source carbon source D-Glucose 0.25mM +2.6 -0.4 +0.1 -1.1 -0.9
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.1 +0.5 -0.6 -1.0 +0.4
motility inner cut, LB soft agar motility assay +2.2 +0.1 -0.3 -0.8 -0.1
predation 6 hr rep2_HD100_10fold_dilution_grewON +1.7 -0.2 -0.3 +1.3 -0.8
carbon source carbon source D-Glucose 5mM +1.1 -0.2 -0.6 +1.0 +1.2
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