Fitness for 5 genes in Escherichia coli BW25113

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500 ntsufS and sufD overlap by 4 nucleotidessufD and sufC overlap by 26 nucleotidessufC and ynhE are separated by 9 nucleotidesynhE and sufA are separated by 8 nucleotides b1680: sufS - selenocysteine lyase (NCBI), at 1,757,327 to 1,758,547 sufS b1681: sufD - component of SufBCD complex (NCBI), at 1,758,544 to 1,759,815 sufD b1682: sufC - cysteine desulfurase ATPase component (NCBI), at 1,759,790 to 1,760,536 sufC b1683: ynhE - orf, hypothetical protein (VIMSS), at 1,760,546 to 1,762,033 ynhE b1684: sufA - iron-sulfur cluster assembly scaffold protein (NCBI), at 1,762,042 to 1,762,410 sufA
Group Condition b1680 b1681 b1682 b1683 b1684
sufS sufD sufC ynhE sufA
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -4.3 -4.6 -4.3 -4.9 +7.4
M9 Acetate 0.5% aerobic -1.7 -1.5 -1.6 -1.5 -0.7
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -3.2 -3.4 -3.9 -3.9 +7.4
stress 3-methyl-2-butenol 1.25 vol% -1.3 -1.6 -1.9 -1.2 -0.8
M9 Acetate 0.5% aerobic -1.4 -1.0 -1.5 -1.5 -0.8
stress 3-methyl-2-butenol 1.25 vol% -1.2 -1.4 -1.6 -1.2 -0.4
phage Δdbn_10 -0.8 -0.8 +0.2 +0.3 -4.8
carbon source Ying_AminoAcid20 rep B; time point 3 -1.3 -1.4 -1.5 -1.2 -0.4
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -1.4 -1.0 -1.3 -1.1 -0.8
carbon source carbon source D-Glucose 0.25mM -2.3 -0.1 -0.3 -0.4 -1.9
carbon source Ying_AminoAcid20 rep C; time point 3 -1.2 -1.1 -1.1 -0.9 -0.7
supernatant LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x -1.1 -1.1 -1.1 -1.2 -0.5
carbon source Ying_AminoAcid20 rep A; time point 3 -1.5 -1.1 -0.9 -1.1 -0.2
control PBS_cage_2_mouse_1_Cecum_40 hr -0.5 +0.0 -0.6 -0.5 -3.2
supernatant LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x -1.1 -0.9 -1.1 -1.0 -0.0
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -1.1 -0.8 +0.1 -1.0 -1.3
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM -0.3 -0.5 -0.5 -0.3 -2.5
stress octanol 0.08 vol% -0.3 +0.0 +0.2 +0.2 -3.9
phage Ste41 -0.3 +0.1 -1.8 -0.9 -0.7
stress Aluminum chloride 5 mM +0.3 -0.2 -0.1 -0.5 -2.7
control NoBALOControl +0.7 -0.2 +0.3 +0.0 -3.8
balo Mixed culture, Halobacteriovorax-1 +0.3 +0.2 +0.3 +0.6 -2.4
carbon source D-Trehalose (C) +0.5 +0.4 +0.5 +0.4 -2.0
nitrogen source Ammonium chloride (N) +0.6 +0.7 +1.2 +0.6 -1.2
nitrogen source L-Glutamine (N) +0.5 +1.0 +0.7 +0.8 -0.8
nitrogen source Cytidine (N) +0.9 +0.7 +0.9 +0.6 -0.7
vitamin M9 with Pyridoxine HCl 20 nM and 4-Deoxypyridoxine 0.25 uM +1.0 +1.0 +0.7 +0.7 -0.3
vitamin M9 with Pyridoxine HCl 20 nM and 4-Deoxypyridoxine 0.25 uM +1.0 +1.0 +0.6 +0.8 -0.3
vitamin M9 with Pyridoxine HCl 20 nM and 4-Deoxypyridoxine 0.25 uM +1.3 +1.1 +1.2 +0.9 -0.4
vitamin M9 with Pyridoxine HCl 20 nM and 4-Deoxypyridoxine 0.25 uM +1.2 +1.2 +0.7 +0.8 +0.3
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