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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
sufS and sufD overlap by 4 nucleotides
sufD and sufC overlap by 26 nucleotides
sufC and ynhE are separated by 9 nucleotides
ynhE and sufA are separated by 8 nucleotides
b1680: sufS - selenocysteine lyase (NCBI), at 1,757,327 to 1,758,547
sufS
b1681: sufD - component of SufBCD complex (NCBI), at 1,758,544 to 1,759,815
sufD
b1682: sufC - cysteine desulfurase ATPase component (NCBI), at 1,759,790 to 1,760,536
sufC
b1683: ynhE - orf, hypothetical protein (VIMSS), at 1,760,546 to 1,762,033
ynhE
b1684: sufA - iron-sulfur cluster assembly scaffold protein (NCBI), at 1,762,042 to 1,762,410
sufA
Group
Condition
b1680
b1681
b1682
b1683
b1684
sufS
sufD
sufC
ynhE
sufA
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-4.3
-4.6
-4.3
-4.9
+7.4
M9 Acetate 0.5% aerobic
-1.7
-1.5
-1.6
-1.5
-0.7
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-3.2
-3.4
-3.9
-3.9
+7.4
stress
3-methyl-2-butenol 1.25 vol%
-1.3
-1.6
-1.9
-1.2
-0.8
M9 Acetate 0.5% aerobic
-1.4
-1.0
-1.5
-1.5
-0.8
stress
3-methyl-2-butenol 1.25 vol%
-1.2
-1.4
-1.6
-1.2
-0.4
phage
Δdbn_10
-0.8
-0.8
+0.2
+0.3
-4.8
carbon source
Ying_AminoAcid20 rep B; time point 3
-1.3
-1.4
-1.5
-1.2
-0.4
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-1.4
-1.0
-1.3
-1.1
-0.8
carbon source
carbon source D-Glucose 0.25mM
-2.3
-0.1
-0.3
-0.4
-1.9
carbon source
Ying_AminoAcid20 rep C; time point 3
-1.2
-1.1
-1.1
-0.9
-0.7
supernatant
LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
-1.1
-1.1
-1.1
-1.2
-0.5
carbon source
Ying_AminoAcid20 rep A; time point 3
-1.5
-1.1
-0.9
-1.1
-0.2
control
PBS_cage_2_mouse_1_Cecum_40 hr
-0.5
+0.0
-0.6
-0.5
-3.2
supernatant
LB 0.5x with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.5x
-1.1
-0.9
-1.1
-1.0
-0.0
stress
LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-1.1
-0.8
+0.1
-1.0
-1.3
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
-0.3
-0.5
-0.5
-0.3
-2.5
stress
octanol 0.08 vol%
-0.3
+0.0
+0.2
+0.2
-3.9
phage
Ste41
-0.3
+0.1
-1.8
-0.9
-0.7
stress
Aluminum chloride 5 mM
+0.3
-0.2
-0.1
-0.5
-2.7
control
NoBALOControl
+0.7
-0.2
+0.3
+0.0
-3.8
balo
Mixed culture, Halobacteriovorax-1
+0.3
+0.2
+0.3
+0.6
-2.4
carbon source
D-Trehalose (C)
+0.5
+0.4
+0.5
+0.4
-2.0
nitrogen source
Ammonium chloride (N)
+0.6
+0.7
+1.2
+0.6
-1.2
nitrogen source
L-Glutamine (N)
+0.5
+1.0
+0.7
+0.8
-0.8
nitrogen source
Cytidine (N)
+0.9
+0.7
+0.9
+0.6
-0.7
vitamin
M9 with Pyridoxine HCl 20 nM and 4-Deoxypyridoxine 0.25 uM
+1.0
+1.0
+0.7
+0.7
-0.3
vitamin
M9 with Pyridoxine HCl 20 nM and 4-Deoxypyridoxine 0.25 uM
+1.0
+1.0
+0.6
+0.8
-0.3
vitamin
M9 with Pyridoxine HCl 20 nM and 4-Deoxypyridoxine 0.25 uM
+1.3
+1.1
+1.2
+0.9
-0.4
vitamin
M9 with Pyridoxine HCl 20 nM and 4-Deoxypyridoxine 0.25 uM
+1.2
+1.2
+0.7
+0.8
+0.3
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