Fitness for 5 genes in Escherichia coli BW25113

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500 ntydhP and ynhF are separated by 165 nucleotidesynhF and purR are separated by 298 nucleotidespurR and ydhB overlap by 4 nucleotidesydhB and ydhC are separated by 112 nucleotides b1657: ydhP - predicted transporter (NCBI), at 1,734,145 to 1,735,314 ydhP b4602: ynhF - hypothetical protein (RefSeq), at 1,735,480 to 1,735,569 ynhF b1658: purR - DNA-binding transcriptional repressor, hypoxanthine-binding (NCBI), at 1,735,868 to 1,736,893 purR b1659: ydhB - predicted DNA-binding transcriptional regulator (NCBI), at 1,736,890 to 1,737,822 ydhB b1660: ydhC - predicted transporter (NCBI), at 1,737,935 to 1,739,146 ydhC
Group Condition b1657 b4602 b1658 b1659 b1660
ydhP ynhF purR ydhB ydhC
motility outer cut, LB soft agar motility assay -0.7 N.D. -2.8 -1.4 -1.0
stress Benzalkonium Chloride 0.004 mg/ml +0.3 N.D. -3.9 -2.1 -0.3
carbon source Ying_Nucleobase14 rep C; time point 3 +0.0 N.D. -1.3 -1.6 -1.7
carbon source Ying_Nucleobase14 rep B; time point 3 +0.4 N.D. -1.2 -2.1 -1.3
carbon source Ying_Nucleobase14 rep A; time point 3 -0.0 N.D. -1.3 -1.1 -1.7
carbon source Ying_Nucleobase14 rep B; time point 2 +0.3 N.D. -0.9 -1.8 -1.6
stress LB Anaerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.0 N.D. +0.3 -1.9 -2.3
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.1 N.D. -3.9 -0.1 +0.1
stress Trimethoprim 0.0025 mM -0.2 N.D. -3.3 -0.4 +0.3
carbon source Ying_Nucleobase14 rep A; time point 2 -0.1 N.D. -0.8 -1.1 -1.7
carbon source Ying_Nucleobase14 rep C; time point 2 +0.2 N.D. -1.1 -1.4 -1.1
carbon source Adenosine 0.3742 mM (C) +0.1 N.D. -0.6 -1.7 -1.2
carbon source Adenosine 0.3742 mM (C) -0.0 N.D. -0.4 -1.3 -1.5
carbon source Inosine 0.3728 mM (C) +0.2 N.D. -0.6 -1.7 -1.0
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.1 N.D. -2.4 -0.3 -0.5
phage Lambda_ci1857 -0.2 N.D. -2.0 -0.9 +0.0
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.2 N.D. -2.1 -0.8 +0.3
stress octanol 0.08 vol% -0.4 N.D. -3.5 +0.9 +0.3
carbon source Guanosine 0.35306 mM (C) +0.4 N.D. -0.5 -1.0 -1.4
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.2 N.D. -3.4 -0.0 +1.0
phage RB68 +0.4 N.D. -2.2 +0.6 +0.3
stress acetate 140 mM +0.4 N.D. -1.7 +0.3 +0.7
phage Ste41 -0.5 N.D. -1.3 +2.2 +0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.2 N.D. +2.0 -0.3 -0.6
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.1 N.D. +2.0 -1.0 +0.9
plasmid transformation vcDART vector transformation; agar assay +0.3 N.D. +2.5 -0.3 -0.3
plasmid transformation vcDART vector transformation; agar assay +0.2 N.D. +2.8 -0.3 +0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.4 N.D. +2.3 -0.0 +0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.3 N.D. +1.6 +1.1 +0.4
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 N.D. +2.8 +0.2 +0.4
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