Fitness for 5 genes in Escherichia coli BW25113

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500 ntgloA and rnt are separated by 102 nucleotidesrnt and lhr are separated by 92 nucleotideslhr and ydhD are separated by 50 nucleotidesydhD and ydhO are separated by 333 nucleotides b1651: gloA - glyoxalase I, Ni-dependent (NCBI), at 1,725,861 to 1,726,268 gloA b1652: rnt - ribonuclease T (NCBI), at 1,726,371 to 1,727,018 rnt b1653: lhr - predicted ATP-dependent helicase (NCBI), at 1,727,111 to 1,731,727 lhr b1654: ydhD - hypothetical protein (NCBI), at 1,731,778 to 1,732,125 ydhD b1655: ydhO - predicted lipoprotein (NCBI), at 1,732,459 to 1,733,274 ydhO
Group Condition b1651 b1652 b1653 b1654 b1655
gloA rnt lhr ydhD ydhO
stress methylglyoxal 0.008 vol% -4.7 N.D. +0.2 N.D. N.D.
stress LB Anaerobic with Cephalothin 0.0000078125 mM -2.5 N.D. +0.2 N.D. N.D.
phage Ste41 -1.3 N.D. -0.7 N.D. N.D.
stress Ciprofloxacin Hydrochloride 0.0024 mM -1.9 N.D. -0.0 N.D. N.D.
stress Doxycycline hyclate 0.001 mg/ml -2.0 N.D. +0.1 N.D. N.D.
phage N4_phage 0.009375 MOI TC1 -1.0 N.D. -0.3 N.D. N.D.
phage Ste41 -0.5 N.D. -0.7 N.D. N.D.
stress 5-Hydroxymethylfurfural 20 mM -1.2 N.D. +0.0 N.D. N.D.
stress 3-methyl-1-butanol 1.25 vol% -1.3 N.D. +0.2 N.D. N.D.
stress Benzalkonium Chloride 0.004 mg/ml -1.2 N.D. +0.1 N.D. N.D.
predation 3 hr rep1_Tiberius_100fold_dilution -1.0 N.D. +0.3 N.D. N.D.
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -1.0 N.D. +0.2 N.D. N.D.
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM -0.9 N.D. +0.2 N.D. N.D.
phage N4_phage 0.00009375 MOI TC5 -0.9 N.D. +0.3 N.D. N.D.
stress Cisplatin 0.1 mg/ml +0.3 N.D. -0.9 N.D. N.D.
phage T7_phage 0.0015625 MOI TC2 +1.2 N.D. -0.9 N.D. N.D.
stress LB Aerobic with Tetracycline 0.0000003125 mM +1.0 N.D. +0.0 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.7 N.D. +0.4 N.D. N.D.
stress LB Aerobic with Doxycycline hyclate 0.0000015625 mM +0.8 N.D. +0.3 N.D. N.D.
stress LB Aerobic with Novobiocin 0.0000625 mM +1.0 N.D. +0.1 N.D. N.D.
stress LB Aerobic with Zinc Pyrithione 0.00000125 mM +1.0 N.D. +0.2 N.D. N.D.
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.9 N.D. +0.3 N.D. N.D.
nophagecontrol R1_N4_T1_phage0 +1.1 N.D. +0.2 N.D. N.D.
stress LB Aerobic with Zinc Pyrithione 0.00000125 mM +1.1 N.D. +0.2 N.D. N.D.
stress LB Aerobic with Benzethonium chloride 0.000015625 mM +1.1 N.D. +0.2 N.D. N.D.
stress LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM +1.2 N.D. +0.3 N.D. N.D.
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +1.3 N.D. +0.3 N.D. N.D.
phage N4_phage 0.009375 MOI TC2 +1.3 N.D. +0.3 N.D. N.D.
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +1.5 N.D. +0.3 N.D. N.D.
stress Trimethoprim 0.005 mM +1.8 N.D. +0.2 N.D. N.D.
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