Fitness for 5 genes in Escherichia coli BW25113

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500 ntpdxY and tyrS are separated by 58 nucleotidestyrS and pdxH are separated by 128 nucleotidespdxH and ydhA are separated by 58 nucleotidesydhA and ydhH are separated by 97 nucleotides b1636: pdxY - pyridoxine kinase (NCBI), at 1,713,050 to 1,713,913 pdxY b1637: tyrS - tyrosyl-tRNA synthetase (NCBI), at 1,713,972 to 1,715,246 tyrS b1638: pdxH - pyridoxamine 5'-phosphate oxidase (NCBI), at 1,715,375 to 1,716,031 pdxH b1639: ydhA - orf, hypothetical protein (VIMSS), at 1,716,090 to 1,716,419 ydhA b1640: ydhH - anhydro-N-acetylmuramic acid kinase (NCBI), at 1,716,517 to 1,717,626 ydhH
Group Condition b1636 b1637 b1638 b1639 b1640
pdxY tyrS pdxH ydhA ydhH
stress Cephalothin 0.01 mg/ml -0.7 N.D. N.D. -0.1 -1.4
phage RB68 -0.4 N.D. N.D. -1.0 -0.7
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM -0.4 N.D. N.D. -1.5 +0.1
stress Ciprofloxacin Hydrochloride 0.0003 mM +0.2 N.D. N.D. -0.1 -1.9
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.3 N.D. N.D. -0.1 -1.2
carbon source Dodecanol and Rhamnolipid (C) -0.2 N.D. N.D. -1.2 -0.1
phage Ste41 +0.6 N.D. N.D. -0.8 -0.9
gfp_control GFP_control +0.2 N.D. N.D. -0.6 -0.7
phage Lambda_ci1857 +0.3 N.D. N.D. -0.1 -1.2
stress Aluminum chloride 5 mM -0.9 N.D. N.D. +0.7 -0.3
stress 3-methyl-1-butanol 1.25 vol% -0.3 N.D. N.D. -1.0 +0.7
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.4 N.D. N.D. -0.9 +0.2
stress Bacitracin 1 mg/ml -0.8 N.D. N.D. +0.9 +0.0
carbon source carbon source D-Mannose 1mM +0.1 N.D. N.D. -0.7 +0.7
predation 6 hr rep2_HD100_10fold_dilution_grewON +0.6 N.D. N.D. +0.5 -1.0
control PBS_cage_1_mouse_1_Cecum_40 hr +1.5 N.D. N.D. -0.2 -0.8
motility inner cut, LB soft agar motility assay -0.1 N.D. N.D. +1.1 -0.5
stress 1-ethyl-3-methylimidazolium acetate 60 mM -0.5 N.D. N.D. +0.9 +0.2
stress Dimethyl Sulfoxide 10 vol% -0.4 N.D. N.D. +0.3 +0.8
stress Thallium(I) acetate 0.0625 mg/ml -0.4 N.D. N.D. +0.5 +0.7
stress 1-ethyl-3-methylimidazolium chloride 40 mM -0.3 N.D. N.D. +0.5 +0.8
carbon source L-Malic (C) -0.1 N.D. N.D. +0.6 +0.9
carbon source a-Ketoglutaric (C) +0.3 N.D. N.D. +0.5 +0.7
stress LB Aerobic with Trimethoprim 0.00000125 mM -0.1 N.D. N.D. +0.6 +1.0
stress Chloramphenicol 0.0025 mg/ml -0.4 N.D. N.D. +1.2 +0.8
stress Fusidic 0.1 mg/ml -0.1 N.D. N.D. +1.1 +0.6
carbon source carbon source D-Glucose 0.25mM +0.8 N.D. N.D. +0.2 +0.7
stress Benzalkonium Chloride 0.004 mg/ml +0.7 N.D. N.D. +0.4 +0.8
carbon source carbon source D-Mannose 5mM +0.9 N.D. N.D. +0.6 +0.5
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.4 N.D. N.D. +1.7 -0.1
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