Fitness for 5 genes in Escherichia coli BW25113

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500 nttppB and gst are separated by 105 nucleotidesgst and pdxY are separated by 43 nucleotidespdxY and tyrS are separated by 58 nucleotidestyrS and pdxH are separated by 128 nucleotides b1634: tppB - putative tripeptide transporter permease (NCBI), at 1,710,793 to 1,712,295 tppB b1635: gst - glutathionine S-transferase (NCBI), at 1,712,401 to 1,713,006 gst b1636: pdxY - pyridoxine kinase (NCBI), at 1,713,050 to 1,713,913 pdxY b1637: tyrS - tyrosyl-tRNA synthetase (NCBI), at 1,713,972 to 1,715,246 tyrS b1638: pdxH - pyridoxamine 5'-phosphate oxidase (NCBI), at 1,715,375 to 1,716,031 pdxH
Group Condition b1634 b1635 b1636 b1637 b1638
tppB gst pdxY tyrS pdxH
stress LB Anaerobic with Cephalothin 0.0000078125 mM -1.9 -1.0 -0.5 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.8 -0.7 -0.2 N.D. N.D.
plasmid transformation vcDART vector transformation; liquid outgrowth assay -2.8 +0.3 +0.1 N.D. N.D.
stress Trimethoprim 0.0025 mM -1.4 -0.8 -0.3 N.D. N.D.
stress D-Cycloserine 0.1 mg/ml -2.4 +0.0 -0.1 N.D. N.D.
stress Trimethoprim 0.00125 mM -1.4 -0.5 -0.4 N.D. N.D.
motility outer cut, LB soft agar motility assay -1.1 -1.0 +0.3 N.D. N.D.
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM -0.9 -0.5 -0.4 N.D. N.D.
stress LB Anaerobic with Ceftriaxone hemi(heptahydrate) 0.000000625 mM -0.9 -0.7 -0.2 N.D. N.D.
stress LB Anaerobic with Levofloxacin 0.0000000625 mM -1.5 -0.2 +0.1 N.D. N.D.
stress Cephalothin 0.01 mg/ml -1.1 +0.1 -0.7 N.D. N.D.
plasmid transformation mariner vector transformation; liquid outgrowth assay -2.4 +0.7 +0.1 N.D. N.D.
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.8 -1.0 +0.4 N.D. N.D.
micoeukaryotes Tetrahymena sp., Mixed culture-1 -1.4 -0.5 +0.5 N.D. N.D.
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM -1.0 -0.7 +0.5 N.D. N.D.
carbon source carbon source D-Mannose 5mM -1.4 -0.7 +0.9 N.D. N.D.
stress LB Anaerobic with Zinc Pyrithione 0.00000125 mM -1.1 -0.5 +0.4 N.D. N.D.
stress Aluminum chloride 5 mM +1.3 -1.1 -0.9 N.D. N.D.
motility outer cut, LB soft agar motility assay -1.0 -0.3 +0.7 N.D. N.D.
carbon source Ying_Nucleobase14 rep B; time point 3 +0.6 -0.6 -0.6 N.D. N.D.
phage Ste41 -1.1 +0.7 -0.2 N.D. N.D.
stress Benzalkonium Chloride 0.004 mg/ml -1.2 -0.0 +0.7 N.D. N.D.
protonated/deuterated protonated glucose in D2O +0.8 -0.6 -0.4 N.D. N.D.
carbon source carbon source D-Glucose 5mM +0.9 -0.5 -0.5 N.D. N.D.
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +1.3 +0.2 -0.3 N.D. N.D.
carbon source carbon source D-Mannose 5mM +0.7 -0.2 +0.8 N.D. N.D.
control PBS_cage_1_mouse_1_Cecum_40 hr +0.1 +0.2 +1.5 N.D. N.D.
phage phage +0.9 +0.7 +0.4 N.D. N.D.
phage T7_phage 0.0015625 MOI TC1 +0.9 +0.9 +0.5 N.D. N.D.
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.9 +1.3 +0.4 N.D. N.D.
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