Fitness for 5 genes in Escherichia coli BW25113

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500 ntrsxD and rsxG are separated by 3 nucleotidesrsxG and rsxE are separated by 3 nucleotidesrsxE and nth overlap by 1 nucleotidesnth and tppB are separated by 610 nucleotides b1630: rsxD - electron transport complex protein RnfD (NCBI), at 1,707,166 to 1,708,224 rsxD b1631: rsxG - electron transport complex protein RnfG (NCBI), at 1,708,228 to 1,708,848 rsxG b1632: rsxE - NADH-ubiquinone oxidoreductase (NCBI), at 1,708,852 to 1,709,547 rsxE b1633: nth - DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) (NCBI), at 1,709,547 to 1,710,182 nth b1634: tppB - putative tripeptide transporter permease (NCBI), at 1,710,793 to 1,712,295 tppB
Group Condition b1630 b1631 b1632 b1633 b1634
rsxD rsxG rsxE nth tppB
plasmid transformation vcDART vector transformation; liquid outgrowth assay -3.3 N.D. -2.1 -0.4 -0.7
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.0 N.D. -1.7 -0.7 -2.8
phage Ste41 -1.0 N.D. -1.6 -2.0 -1.1
stress 2-nitrobenzenesulfonyl fluoride -2.5 N.D. -1.9 -0.3 -0.0
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.7 N.D. -1.4 +0.5 -1.8
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.2 N.D. -1.2 -0.5 -1.0
phage T7_phage 0.00015625 MOI TC4 -2.1 N.D. -2.2 +0.1 +0.3
phage T7_phage 0.00015625 MOI TC2 -1.7 N.D. -2.3 -0.1 +0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.7 N.D. -0.9 +0.5 -2.4
carbon source Glycolic Acid (C) -2.2 N.D. -1.2 +0.3 +0.5
phage T7_phage 0.0015625 MOI TC1 -1.8 N.D. -1.8 +0.2 +0.9
protonated/deuterated protonated glucose in D2O -1.9 N.D. -1.3 +0.1 +0.7
stress LB Anaerobic with Cephalothin 0.0000078125 mM +1.1 N.D. +1.2 +0.1 -1.9
stress LB Anaerobic with Levofloxacin 0.0000000625 mM +0.8 N.D. +0.8 +0.7 -1.5
stress LB Anaerobic with Cephalothin 0.0000078125 mM +1.4 N.D. +1.2 -0.4 -1.3
stress Trimethoprim 0.0025 mM +1.9 N.D. +2.0 -1.3 -1.4
stress Cephalothin 0.01 mg/ml +1.7 N.D. +1.5 +0.1 -1.1
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM +1.4 N.D. +1.6 +0.4 -0.8
stress Trimethoprim 0.00125 mM +2.1 N.D. +2.0 -0.0 -1.4
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +1.2 N.D. +1.4 +0.8 -0.4
stress Trimethoprim 0.005 mM +2.1 N.D. +1.5 +0.0 -0.2
stress Doxycycline hyclate 0.001 mg/ml +1.7 N.D. +1.7 +0.3 -0.1
stress octanol 0.08 vol% +2.0 N.D. +2.6 -1.1 +0.3
stress Tetracycline 0.0008 mg/ml +2.4 N.D. +2.2 -0.4 -0.3
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM +2.0 N.D. +1.9 +0.6 -0.6
stress Chloramphenicol 0.001 mg/ml +1.6 N.D. +1.9 +0.5 -0.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +1.8 N.D. +1.6 +1.1 -0.5
stress Chloramphenicol 0.0025 mg/ml +2.1 N.D. +2.3 +0.3 +0.1
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +1.1 N.D. +1.5 +1.0 +1.3
stress Benzalkonium Chloride 0.004 mg/ml +3.8 N.D. +4.0 +0.7 -1.2
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