Fitness for 5 genes in Escherichia coli BW25113

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500 ntmalX and malY are separated by 9 nucleotidesmalY and add are separated by 103 nucleotidesadd and b1624 are separated by 33 nucleotidesb1624 and blr are separated by 242 nucleotides b1621: malX - fused maltose and glucose-specific PTS enzymes: IIB component -! IIC component (NCBI), at 1,697,379 to 1,698,971 malX b1622: malY - bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI), at 1,698,981 to 1,700,153 malY b1623: add - adenosine deaminase (NCBI), at 1,700,257 to 1,701,258 add b1624: b1624 - orf, hypothetical protein (VIMSS), at 1,701,292 to 1,702,332 b1624 b4409: blr - beta-lactam resistance membrane protein (NCBI), at 1,702,575 to 1,702,700 blr
Group Condition b1621 b1622 b1623 b1624 b4409
malX malY add b1624 blr
phage T7_phage 0.0015625 MOI TC2 -0.7 -2.8 +0.6 +0.1 -0.9
stress Benzalkonium Chloride 0.004 mg/ml -1.1 -0.1 -1.2 -0.5 -0.3
phage RhdML5 -0.5 -0.9 -1.2 -0.6 +0.4
stress Trimethoprim 0.005 mM -0.5 -0.6 -0.1 -0.7 -0.4
stress Cisplatin 0.1 mg/ml -0.6 -0.9 +0.2 -0.3 -0.5
stress Trimethoprim 0.0025 mM -0.4 -0.4 -1.2 -0.2 +0.1
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.1 -0.5 -0.4 -0.4 -0.9
phage T7_phage 0.00015625 MOI TC4 +0.0 -0.7 -0.7 +0.2 -0.6
no phage control CTR -0.9 +0.0 -0.8 +0.8 -0.9
stress Ciprofloxacin Hydrochloride 0.0003 mM -0.3 -0.2 -0.6 -0.9 +0.2
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.1 -0.7 -0.6 +0.5 -1.0
phage N4_phage 0.09375 MOI TC1 -1.2 -0.3 +0.2 +0.4 -0.5
phage RB68 -0.9 -0.8 +0.6 +1.0 -1.1
stress 3-methyl-1-butanol 1.25 vol% -0.6 -0.4 -0.5 +0.7 -0.3
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.5 -0.4 -0.5 +0.2 -0.7
motility outer cut, LB soft agar motility assay +0.8 -0.3 +0.0 -0.3 -0.7
phage RB68 -0.7 +0.1 +0.5 +0.9 -1.3
stress LB Aerobic with Irgasan 0.0000003125 mM -0.3 +0.8 -1.4 -0.2 +0.6
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.9 +0.3 +0.1 -0.6 -0.7
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.3 -0.4 -0.9 -0.1 +1.2
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM +0.3 -0.8 -0.4 +0.3 +0.8
stress Chlorite 1.25 mM -0.3 +0.5 -0.9 -0.3 +1.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.4 +0.7 +1.2 -0.1 -0.9
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.1 +0.5 -2.1 +0.7 +1.6
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM +0.4 +0.5 +0.3 +0.5 -0.7
phage NpK -0.2 +0.4 +1.0 +0.4 -0.4
stress 3-methyl-1-butanol 1.25 vol% +0.7 -0.7 +0.4 +0.4 +0.3
phage Ste41 +0.2 +0.4 +0.2 +1.3 -0.6
phage PoolT9D25 -0.5 +0.7 +0.8 +0.4 +0.4
stress LB Aerobic with Ramoplanin 0.000125 mM -0.1 +0.8 +0.7 -0.2 +0.9
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