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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
malX and malY are separated by 9 nucleotides
malY and add are separated by 103 nucleotides
add and b1624 are separated by 33 nucleotides
b1624 and blr are separated by 242 nucleotides
b1621: malX - fused maltose and glucose-specific PTS enzymes: IIB component -! IIC component (NCBI), at 1,697,379 to 1,698,971
malX
b1622: malY - bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI), at 1,698,981 to 1,700,153
malY
b1623: add - adenosine deaminase (NCBI), at 1,700,257 to 1,701,258
add
b1624: b1624 - orf, hypothetical protein (VIMSS), at 1,701,292 to 1,702,332
b1624
b4409: blr - beta-lactam resistance membrane protein (NCBI), at 1,702,575 to 1,702,700
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Group
Condition
b1621
b1622
b1623
b1624
b4409
malX
malY
add
b1624
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phage
T7_phage 0.0015625 MOI TC2
-0.7
-2.8
+0.6
+0.1
-0.9
stress
Benzalkonium Chloride 0.004 mg/ml
-1.1
-0.1
-1.2
-0.5
-0.3
phage
RhdML5
-0.5
-0.9
-1.2
-0.6
+0.4
stress
Trimethoprim 0.005 mM
-0.5
-0.6
-0.1
-0.7
-0.4
stress
Cisplatin 0.1 mg/ml
-0.6
-0.9
+0.2
-0.3
-0.5
stress
Trimethoprim 0.0025 mM
-0.4
-0.4
-1.2
-0.2
+0.1
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.1
-0.5
-0.4
-0.4
-0.9
phage
T7_phage 0.00015625 MOI TC4
+0.0
-0.7
-0.7
+0.2
-0.6
no phage control
CTR
-0.9
+0.0
-0.8
+0.8
-0.9
stress
Ciprofloxacin Hydrochloride 0.0003 mM
-0.3
-0.2
-0.6
-0.9
+0.2
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.1
-0.7
-0.6
+0.5
-1.0
phage
N4_phage 0.09375 MOI TC1
-1.2
-0.3
+0.2
+0.4
-0.5
phage
RB68
-0.9
-0.8
+0.6
+1.0
-1.1
stress
3-methyl-1-butanol 1.25 vol%
-0.6
-0.4
-0.5
+0.7
-0.3
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.5
-0.4
-0.5
+0.2
-0.7
motility
outer cut, LB soft agar motility assay
+0.8
-0.3
+0.0
-0.3
-0.7
phage
RB68
-0.7
+0.1
+0.5
+0.9
-1.3
stress
LB Aerobic with Irgasan 0.0000003125 mM
-0.3
+0.8
-1.4
-0.2
+0.6
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.9
+0.3
+0.1
-0.6
-0.7
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
+0.3
-0.4
-0.9
-0.1
+1.2
stress
LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
+0.3
-0.8
-0.4
+0.3
+0.8
stress
Chlorite 1.25 mM
-0.3
+0.5
-0.9
-0.3
+1.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.4
+0.7
+1.2
-0.1
-0.9
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.1
+0.5
-2.1
+0.7
+1.6
stress
LB Anaerobic with Phenazine ethosulfate 0.000125 mM
+0.4
+0.5
+0.3
+0.5
-0.7
phage
NpK
-0.2
+0.4
+1.0
+0.4
-0.4
stress
3-methyl-1-butanol 1.25 vol%
+0.7
-0.7
+0.4
+0.4
+0.3
phage
Ste41
+0.2
+0.4
+0.2
+1.3
-0.6
phage
PoolT9D25
-0.5
+0.7
+0.8
+0.4
+0.4
stress
LB Aerobic with Ramoplanin 0.000125 mM
-0.1
+0.8
+0.7
-0.2
+0.9
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