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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
malI and malX are separated by 174 nucleotides
malX and malY are separated by 9 nucleotides
malY and add are separated by 103 nucleotides
add and b1624 are separated by 33 nucleotides
b1620: malI - DNA-binding transcriptional repressor (NCBI), at 1,696,176 to 1,697,204
malI
b1621: malX - fused maltose and glucose-specific PTS enzymes: IIB component -! IIC component (NCBI), at 1,697,379 to 1,698,971
malX
b1622: malY - bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI), at 1,698,981 to 1,700,153
malY
b1623: add - adenosine deaminase (NCBI), at 1,700,257 to 1,701,258
add
b1624: b1624 - orf, hypothetical protein (VIMSS), at 1,701,292 to 1,702,332
b1624
Group
Condition
b1620
b1621
b1622
b1623
b1624
malI
malX
malY
add
b1624
phage
T7_phage 0.0015625 MOI TC2
-2.0
-0.7
-2.8
+0.6
+0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-4.0
-0.4
+0.1
-0.3
+0.3
carbon source
carbon source D-Mannose 0.5mM
-2.4
-0.3
-0.1
-0.1
-0.5
stress
Trimethoprim 0.005 mM
-1.1
-0.5
-0.6
-0.1
-0.7
stress
LB Aerobic with Irgasan 0.0000003125 mM
-1.7
-0.3
+0.8
-1.4
-0.2
stress
2,4-Dinitrophenol 2 mM
-1.5
+0.0
-0.2
-0.9
+0.1
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-1.4
+0.1
-0.5
-0.4
-0.4
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-1.7
+0.1
-0.7
-0.6
+0.5
stress
Benzalkonium Chloride 0.004 mg/ml
+0.6
-1.1
-0.1
-1.2
-0.5
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-2.8
-0.5
+0.7
+0.0
+0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-3.9
-0.1
+0.4
+1.0
+0.3
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-1.4
+0.1
+0.5
-2.1
+0.7
predation
6 hr rep2_HD100_10fold_dilution_grewON
-1.1
+0.2
-0.6
-0.7
+0.4
phage
Δdbn_10
-1.8
+0.5
-0.0
-0.2
-0.3
stress
Trimethoprim 0.0025 mM
+0.5
-0.4
-0.4
-1.2
-0.2
carbon source
carbon source D-Glucose 0.25mM
-1.3
-0.6
-0.1
+0.4
+0.3
plasmid transformation
vcDART vector transformation; agar assay
-1.3
-0.3
+0.4
+0.3
-0.2
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-1.5
-0.0
+0.6
-0.5
+0.4
phage
RB68
-1.6
-0.7
+0.1
+0.5
+0.9
stress
LB Aerobic with Ramoplanin 0.000125 mM
-1.6
+0.0
+0.6
+0.2
+0.1
stress
LB Anaerobic with Irgasan 0.000000625 mM
-1.2
+0.3
+0.3
+0.5
-0.2
phage
T7_phage 0.00015625 MOI TC3
-1.1
+0.5
+0.2
+0.6
-0.2
phage
RB68
+0.1
-0.9
-0.8
+0.6
+1.0
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.7
-0.4
+0.7
+1.2
-0.1
stress
3-methyl-1-butanol 1.25 vol%
+0.5
+0.7
-0.7
+0.4
+0.4
phage
Ste41
-0.4
+0.2
+0.4
+0.2
+1.3
fr_100_e2
Fr_100_E2
+3.7
+0.0
+0.1
-0.2
+0.0
fr_1000_e3
Fr_1000_E3
+4.1
+0.4
-0.2
+0.2
-0.0
fr_1000_f3
Fr_1000_F3
+5.1
+0.3
-0.2
-0.0
+0.0
fr_100_f2
Fr_100_F2
+6.0
+0.2
+0.0
+0.1
-0.1
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