Fitness for 5 genes in Escherichia coli BW25113

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500 ntmalI and malX are separated by 174 nucleotidesmalX and malY are separated by 9 nucleotidesmalY and add are separated by 103 nucleotidesadd and b1624 are separated by 33 nucleotides b1620: malI - DNA-binding transcriptional repressor (NCBI), at 1,696,176 to 1,697,204 malI b1621: malX - fused maltose and glucose-specific PTS enzymes: IIB component -! IIC component (NCBI), at 1,697,379 to 1,698,971 malX b1622: malY - bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI), at 1,698,981 to 1,700,153 malY b1623: add - adenosine deaminase (NCBI), at 1,700,257 to 1,701,258 add b1624: b1624 - orf, hypothetical protein (VIMSS), at 1,701,292 to 1,702,332 b1624
Group Condition b1620 b1621 b1622 b1623 b1624
malI malX malY add b1624
phage T7_phage 0.0015625 MOI TC2 -2.0 -0.7 -2.8 +0.6 +0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay -4.0 -0.4 +0.1 -0.3 +0.3
carbon source carbon source D-Mannose 0.5mM -2.4 -0.3 -0.1 -0.1 -0.5
stress Trimethoprim 0.005 mM -1.1 -0.5 -0.6 -0.1 -0.7
stress LB Aerobic with Irgasan 0.0000003125 mM -1.7 -0.3 +0.8 -1.4 -0.2
stress 2,4-Dinitrophenol 2 mM -1.5 +0.0 -0.2 -0.9 +0.1
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -1.4 +0.1 -0.5 -0.4 -0.4
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -1.7 +0.1 -0.7 -0.6 +0.5
stress Benzalkonium Chloride 0.004 mg/ml +0.6 -1.1 -0.1 -1.2 -0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay -2.8 -0.5 +0.7 +0.0 +0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay -3.9 -0.1 +0.4 +1.0 +0.3
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM -1.4 +0.1 +0.5 -2.1 +0.7
predation 6 hr rep2_HD100_10fold_dilution_grewON -1.1 +0.2 -0.6 -0.7 +0.4
phage Δdbn_10 -1.8 +0.5 -0.0 -0.2 -0.3
stress Trimethoprim 0.0025 mM +0.5 -0.4 -0.4 -1.2 -0.2
carbon source carbon source D-Glucose 0.25mM -1.3 -0.6 -0.1 +0.4 +0.3
plasmid transformation vcDART vector transformation; agar assay -1.3 -0.3 +0.4 +0.3 -0.2
stress LB Anaerobic with Cephalothin 0.0000078125 mM -1.5 -0.0 +0.6 -0.5 +0.4
phage RB68 -1.6 -0.7 +0.1 +0.5 +0.9
stress LB Aerobic with Ramoplanin 0.000125 mM -1.6 +0.0 +0.6 +0.2 +0.1
stress LB Anaerobic with Irgasan 0.000000625 mM -1.2 +0.3 +0.3 +0.5 -0.2
phage T7_phage 0.00015625 MOI TC3 -1.1 +0.5 +0.2 +0.6 -0.2
phage RB68 +0.1 -0.9 -0.8 +0.6 +1.0
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.7 -0.4 +0.7 +1.2 -0.1
stress 3-methyl-1-butanol 1.25 vol% +0.5 +0.7 -0.7 +0.4 +0.4
phage Ste41 -0.4 +0.2 +0.4 +0.2 +1.3
fr_100_e2 Fr_100_E2 +3.7 +0.0 +0.1 -0.2 +0.0
fr_1000_e3 Fr_1000_E3 +4.1 +0.4 -0.2 +0.2 -0.0
fr_1000_f3 Fr_1000_F3 +5.1 +0.3 -0.2 -0.0 +0.0
fr_100_f2 Fr_100_F2 +6.0 +0.2 +0.0 +0.1 -0.1
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