Fitness for 5 genes in Escherichia coli BW25113

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500 ntuidR and hdhA are separated by 220 nucleotideshdhA and malI are separated by 111 nucleotidesmalI and malX are separated by 174 nucleotidesmalX and malY are separated by 9 nucleotides b1618: uidR - DNA-binding transcriptional repressor (NCBI), at 1,694,486 to 1,695,076 uidR b1619: hdhA - 7-alpha-hydroxysteroid dehydrogenase (NCBI), at 1,695,297 to 1,696,064 hdhA b1620: malI - DNA-binding transcriptional repressor (NCBI), at 1,696,176 to 1,697,204 malI b1621: malX - fused maltose and glucose-specific PTS enzymes: IIB component -! IIC component (NCBI), at 1,697,379 to 1,698,971 malX b1622: malY - bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI), at 1,698,981 to 1,700,153 malY
Group Condition b1618 b1619 b1620 b1621 b1622
uidR hdhA malI malX malY
plasmid transformation mariner vector transformation; liquid outgrowth assay -2.4 +0.1 -3.9 -0.1 +0.4
phage T7_phage 0.0015625 MOI TC2 +0.4 -0.6 -2.0 -0.7 -2.8
stress 2,4-Dinitrophenol 2 mM -3.7 +0.1 -1.5 +0.0 -0.2
stress 2,4-Dinitrophenol 2 mM -3.7 -0.1 -0.8 +0.1 +0.0
carbon source carbon source D-Glucose 0.25mM -2.4 -0.0 -1.3 -0.6 -0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.3 -0.4 -2.8 -0.5 +0.7
plasmid transformation vcDART vector transformation; liquid outgrowth assay -0.2 +0.2 -4.0 -0.4 +0.1
plasmid transformation mariner vector transformation; liquid outgrowth assay -1.6 -0.2 -0.6 -1.0 -0.2
stress Trimethoprim 0.005 mM -1.1 +0.0 -1.1 -0.5 -0.6
carbon source carbon source D-Mannose 0.5mM -0.1 -0.1 -2.4 -0.3 -0.1
phage RB68 -0.7 -0.6 +0.1 -0.9 -0.8
phage RB68 -0.0 -0.6 -1.6 -0.7 +0.1
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.4 +0.4 -1.7 +0.1 -0.7
stress Ciprofloxacin Hydrochloride 0.000156 mM -0.6 -0.1 -0.6 +0.2 -1.0
phage T7_phage 0.00015625 MOI TC3 -1.6 -0.1 -1.1 +0.5 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.3 -0.3 -0.7 -0.4 +0.7
predation 6 hr rep2_HD100_10fold_dilution_grewON +0.5 -0.6 -1.1 +0.2 -0.6
carbon source carbon source D-Glucose 0.25mM -2.4 +0.7 +0.4 +0.3 -0.3
phage N4_phage 0.00009375 MOI TC3 +0.7 +0.1 -1.2 -0.3 -0.3
stress LB Aerobic with Irgasan 0.0000003125 mM +0.9 -0.4 -1.7 -0.3 +0.8
stress LB Anaerobic with Cephalothin 0.0000078125 mM +0.6 -0.5 -1.5 -0.0 +0.6
phage Δdbn_10 +0.5 +0.2 -1.8 +0.5 -0.0
stress LB Aerobic with Ramoplanin 0.000125 mM +1.0 -0.4 -1.6 +0.0 +0.6
stress Benzalkonium Chloride 0.004 mg/ml -0.5 +0.7 +0.6 -1.1 -0.1
stress LB Anaerobic with Amoxicillin 0.00000390625 mM +0.6 +0.4 -1.1 +0.2 +0.4
predation 6 hr rep1_HD100_10fold_dilution_grewON +0.5 +0.4 -0.5 +0.9 +0.3
fr_100_e2 Fr_100_E2 -0.9 -0.1 +3.7 +0.0 +0.1
fr_1000_e3 Fr_1000_E3 -0.7 -0.2 +4.1 +0.4 -0.2
fr_1000_f3 Fr_1000_F3 -0.9 -0.1 +5.1 +0.3 -0.2
fr_100_f2 Fr_100_F2 -0.5 -0.1 +6.0 +0.2 +0.0
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