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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
uidR and hdhA are separated by 220 nucleotides
hdhA and malI are separated by 111 nucleotides
malI and malX are separated by 174 nucleotides
malX and malY are separated by 9 nucleotides
b1618: uidR - DNA-binding transcriptional repressor (NCBI), at 1,694,486 to 1,695,076
uidR
b1619: hdhA - 7-alpha-hydroxysteroid dehydrogenase (NCBI), at 1,695,297 to 1,696,064
hdhA
b1620: malI - DNA-binding transcriptional repressor (NCBI), at 1,696,176 to 1,697,204
malI
b1621: malX - fused maltose and glucose-specific PTS enzymes: IIB component -! IIC component (NCBI), at 1,697,379 to 1,698,971
malX
b1622: malY - bifunctional beta-cystathionase, PLP-dependent/ regulator of maltose regulon (NCBI), at 1,698,981 to 1,700,153
malY
Group
Condition
b1618
b1619
b1620
b1621
b1622
uidR
hdhA
malI
malX
malY
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-2.4
+0.1
-3.9
-0.1
+0.4
phage
T7_phage 0.0015625 MOI TC2
+0.4
-0.6
-2.0
-0.7
-2.8
stress
2,4-Dinitrophenol 2 mM
-3.7
+0.1
-1.5
+0.0
-0.2
stress
2,4-Dinitrophenol 2 mM
-3.7
-0.1
-0.8
+0.1
+0.0
carbon source
carbon source D-Glucose 0.25mM
-2.4
-0.0
-1.3
-0.6
-0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-1.3
-0.4
-2.8
-0.5
+0.7
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.2
+0.2
-4.0
-0.4
+0.1
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-1.6
-0.2
-0.6
-1.0
-0.2
stress
Trimethoprim 0.005 mM
-1.1
+0.0
-1.1
-0.5
-0.6
carbon source
carbon source D-Mannose 0.5mM
-0.1
-0.1
-2.4
-0.3
-0.1
phage
RB68
-0.7
-0.6
+0.1
-0.9
-0.8
phage
RB68
-0.0
-0.6
-1.6
-0.7
+0.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-0.4
+0.4
-1.7
+0.1
-0.7
stress
Ciprofloxacin Hydrochloride 0.000156 mM
-0.6
-0.1
-0.6
+0.2
-1.0
phage
T7_phage 0.00015625 MOI TC3
-1.6
-0.1
-1.1
+0.5
+0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.3
-0.3
-0.7
-0.4
+0.7
predation
6 hr rep2_HD100_10fold_dilution_grewON
+0.5
-0.6
-1.1
+0.2
-0.6
carbon source
carbon source D-Glucose 0.25mM
-2.4
+0.7
+0.4
+0.3
-0.3
phage
N4_phage 0.00009375 MOI TC3
+0.7
+0.1
-1.2
-0.3
-0.3
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.9
-0.4
-1.7
-0.3
+0.8
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.6
-0.5
-1.5
-0.0
+0.6
phage
Δdbn_10
+0.5
+0.2
-1.8
+0.5
-0.0
stress
LB Aerobic with Ramoplanin 0.000125 mM
+1.0
-0.4
-1.6
+0.0
+0.6
stress
Benzalkonium Chloride 0.004 mg/ml
-0.5
+0.7
+0.6
-1.1
-0.1
stress
LB Anaerobic with Amoxicillin 0.00000390625 mM
+0.6
+0.4
-1.1
+0.2
+0.4
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.5
+0.4
-0.5
+0.9
+0.3
fr_100_e2
Fr_100_E2
-0.9
-0.1
+3.7
+0.0
+0.1
fr_1000_e3
Fr_1000_E3
-0.7
-0.2
+4.1
+0.4
-0.2
fr_1000_f3
Fr_1000_F3
-0.9
-0.1
+5.1
+0.3
-0.2
fr_100_f2
Fr_100_F2
-0.5
-0.1
+6.0
+0.2
+0.0
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