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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
pntB and pntA are separated by 10 nucleotides
pntA and ydgH are separated by 523 nucleotides
ydgH and ydgI are separated by 185 nucleotides
ydgI and folM are separated by 36 nucleotides
b1602: pntB - pyridine nucleotide transhydrogenase (NCBI), at 1,672,996 to 1,674,384
pntB
b1603: pntA - NAD(P) transhydrogenase subunit alpha (NCBI), at 1,674,395 to 1,675,927
pntA
b1604: ydgH - hypothetical protein (NCBI), at 1,676,451 to 1,677,395
ydgH
b1605: ydgI - predicted arginine/ornithine antiporter transporter (NCBI), at 1,677,581 to 1,678,963
ydgI
b1606: folM - short chain dehydrogenase (NCBI), at 1,679,000 to 1,679,722
folM
Group
Condition
b1602
b1603
b1604
b1605
b1606
pntB
pntA
ydgH
ydgI
folM
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-0.9
+0.2
-4.2
-2.4
-0.1
stress
octanol 0.08 vol%
+0.5
+0.4
-6.0
-0.8
+0.3
stress
undecene 5 vol%
-0.0
-0.5
-4.7
-0.2
-0.1
stress
undecene 5 vol%
-0.0
+0.0
-4.5
-0.6
-0.0
stress
Benzalkonium Chloride 0.004 mg/ml
-0.3
+0.1
-4.5
-0.4
+0.4
stress
undecene 2.5 vol%
-0.0
-0.3
-3.7
-0.2
+0.1
stress
undecene 2.5 vol%
-0.1
-0.5
-2.6
-0.1
+0.2
stress
decyl alcohol 5 vol%
-0.1
+0.1
-2.8
-0.2
-0.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.1
+0.1
-2.3
-0.4
-0.4
nitrogen source
D-Serine (N)
-1.5
-1.3
+0.1
-0.2
+0.1
phage
T7_phage 0.00015625 MOI TC4
-0.1
+0.7
-2.7
-0.2
-0.5
stress
LB Anaerobic with Benzethonium chloride 0.000015625 mM
-0.1
-0.1
-2.6
-0.0
+0.1
stress
Cisplatin 0.1 mg/ml
-0.2
+0.2
-1.4
-1.1
-0.2
stress
LB Anaerobic with Irgasan 0.000000625 mM
+0.4
-0.1
-2.6
+0.2
+0.1
stress
Trimethoprim 0.005 mM
-0.2
-1.9
-1.0
-0.3
+1.5
stress
LB Aerobic with Irgasan 0.0000003125 mM
-0.5
+0.4
-1.6
+0.1
+0.5
stress
Trimethoprim 0.0025 mM
-0.2
-0.4
-0.8
-1.0
+1.4
stress
Trimethoprim 0.00125 mM
+0.1
+0.3
-1.2
-1.0
+1.1
carbon source
carbon source D-Glucose 5mM
+1.1
-1.6
-0.1
+0.1
+0.5
phage
T7_phage 0.0015625 MOI TC1
+0.4
+1.2
-1.1
-0.1
-0.3
phage
Ste41
-0.9
+0.4
+1.1
+0.5
-0.5
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.7
-0.2
-0.4
-0.5
+1.3
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.3
-0.6
+1.1
-0.5
+0.6
carbon source
carbon source D-Mannose 5mM
+0.7
+0.3
+0.6
-1.1
+0.9
stress
M9 with Methotrexate 1.98 mM 24 hr
-0.1
-0.4
+0.7
-0.4
+1.7
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.0
-0.9
+3.7
-0.1
-0.7
stress
Carbenicillin 0.008 mg/ml
-0.0
-0.7
+2.6
-0.2
+0.4
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
+0.0
-1.2
+4.4
-0.6
+0.0
stress
M9 with Methotrexate 1.98 mM 24 hr
-0.0
-0.1
+0.7
-0.2
+2.9
stress
Cephalothin 0.01 mg/ml
+0.4
+0.2
+5.8
-0.0
-0.1
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