Fitness for 5 genes in Escherichia coli BW25113

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500 ntynfA and ynfB are separated by 134 nucleotidesynfB and speG are separated by 34 nucleotidesspeG and ynfC are separated by 2 nucleotidesynfC and b1586 are separated by 107 nucleotides b1582: ynfA - hypothetical protein (NCBI), at 1,653,371 to 1,653,697 ynfA b1583: ynfB - hypothetical protein (NCBI), at 1,653,832 to 1,654,173 ynfB b1584: speG - spermidine N1-acetyltransferase (NCBI), at 1,654,208 to 1,654,768 speG b1585: ynfC - orf, hypothetical protein (VIMSS), at 1,654,771 to 1,655,481 ynfC b1586: b1586 - orf, hypothetical protein (VIMSS), at 1,655,589 to 1,655,894 b1586
Group Condition b1582 b1583 b1584 b1585 b1586
ynfA ynfB speG ynfC b1586
phage Δdbn_10 -0.4 -4.6 +0.2 -0.4 -0.6
phage N4_phage 0.00009375 MOI TC4 -0.9 -0.9 -0.4 -0.1 -0.5
stress LB Anaerobic with Cephalothin 0.0000078125 mM -3.2 +0.1 +0.2 +0.1 +0.0
phage N4_phage 0.00009375 MOI TC5 -0.7 -2.2 +0.4 -0.1 -0.0
stress 3-methyl-1-butanol 1.25 vol% -0.1 -1.6 -1.0 +0.1 +0.3
phage N4_phage 0.009375 MOI TC2 -2.1 +0.5 -0.3 -0.1 +0.3
stress Trimethoprim 0.005 mM +0.7 -0.7 -1.0 -0.2 -0.4
carbon source carbon source D-Glucose 5mM -1.6 +0.8 +0.2 -1.1 +0.1
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -1.2 -0.6 +0.6 +0.2 -0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay -1.5 +0.2 -0.2 -0.4 +0.5
phage Ste41 -1.0 -0.9 +1.0 +0.0 -0.4
phage N4_phage 0.0009375 MOI TC2 -1.8 -0.1 +0.3 +0.5 -0.1
stress Chloride 750 mM -0.9 -0.5 -0.5 -0.1 +0.9
phage T7_phage 0.0015625 MOI TC2 -0.1 +0.3 +0.7 -0.6 -1.4
carbon source a-Ketoglutaric (C) +0.4 +0.3 -1.7 +0.2 -0.3
carbon source carbon source D-Glucose 0.25mM -1.4 +0.5 -0.9 +0.7 +0.0
phage N4_phage 0.0009375 MOI TC3 +0.6 -1.6 +0.3 -0.2 -0.0
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -1.7 -0.4 +0.3 +0.0 +1.0
control NoBALOControl +0.3 -1.8 +0.4 -0.0 +0.4
phage Lambda_ci1857 +0.4 +0.5 -0.7 +0.2 -1.0
stress Cephalothin 0.01 mg/ml -1.8 +0.3 +0.3 +0.2 +0.8
phage T7_phage 0.00015625 MOI TC4 -0.4 -1.0 +1.2 +0.1 -0.3
stress M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM +0.5 +0.7 -1.3 +0.1 -0.1
stress LB Aerobic with Doxycycline hyclate 0.0000015625 mM -0.6 -0.9 +1.1 +0.4 +0.1
phage R2_N4_T2_phage10 +0.8 -1.1 -0.2 +0.4 +0.4
stress LB Aerobic with Tetracycline 0.0000003125 mM +0.1 -0.8 +0.8 +0.4 +0.7
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -0.4 +1.8 -1.0 +0.6 +0.4
stress 1-ethyl-3-methylimidazolium chloride 80 mM +0.2 +0.8 +1.7 -0.0 +0.2
carbon source Ying_Others16 rep A; time point 2 -0.2 +1.4 +4.8 +0.4 +0.2
carbon source Ying_Others16 rep B; time point 2 +1.0 +1.6 +4.4 +0.2 +0.1
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