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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ynfA and ynfB are separated by 134 nucleotides
ynfB and speG are separated by 34 nucleotides
speG and ynfC are separated by 2 nucleotides
ynfC and b1586 are separated by 107 nucleotides
b1582: ynfA - hypothetical protein (NCBI), at 1,653,371 to 1,653,697
ynfA
b1583: ynfB - hypothetical protein (NCBI), at 1,653,832 to 1,654,173
ynfB
b1584: speG - spermidine N1-acetyltransferase (NCBI), at 1,654,208 to 1,654,768
speG
b1585: ynfC - orf, hypothetical protein (VIMSS), at 1,654,771 to 1,655,481
ynfC
b1586: b1586 - orf, hypothetical protein (VIMSS), at 1,655,589 to 1,655,894
b1586
Group
Condition
b1582
b1583
b1584
b1585
b1586
ynfA
ynfB
speG
ynfC
b1586
phage
Δdbn_10
-0.4
-4.6
+0.2
-0.4
-0.6
phage
N4_phage 0.00009375 MOI TC4
-0.9
-0.9
-0.4
-0.1
-0.5
stress
LB Anaerobic with Cephalothin 0.0000078125 mM
-3.2
+0.1
+0.2
+0.1
+0.0
phage
N4_phage 0.00009375 MOI TC5
-0.7
-2.2
+0.4
-0.1
-0.0
stress
3-methyl-1-butanol 1.25 vol%
-0.1
-1.6
-1.0
+0.1
+0.3
phage
N4_phage 0.009375 MOI TC2
-2.1
+0.5
-0.3
-0.1
+0.3
stress
Trimethoprim 0.005 mM
+0.7
-0.7
-1.0
-0.2
-0.4
carbon source
carbon source D-Glucose 5mM
-1.6
+0.8
+0.2
-1.1
+0.1
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-1.2
-0.6
+0.6
+0.2
-0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.5
+0.2
-0.2
-0.4
+0.5
phage
Ste41
-1.0
-0.9
+1.0
+0.0
-0.4
phage
N4_phage 0.0009375 MOI TC2
-1.8
-0.1
+0.3
+0.5
-0.1
stress
Chloride 750 mM
-0.9
-0.5
-0.5
-0.1
+0.9
phage
T7_phage 0.0015625 MOI TC2
-0.1
+0.3
+0.7
-0.6
-1.4
carbon source
a-Ketoglutaric (C)
+0.4
+0.3
-1.7
+0.2
-0.3
carbon source
carbon source D-Glucose 0.25mM
-1.4
+0.5
-0.9
+0.7
+0.0
phage
N4_phage 0.0009375 MOI TC3
+0.6
-1.6
+0.3
-0.2
-0.0
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-1.7
-0.4
+0.3
+0.0
+1.0
control
NoBALOControl
+0.3
-1.8
+0.4
-0.0
+0.4
phage
Lambda_ci1857
+0.4
+0.5
-0.7
+0.2
-1.0
stress
Cephalothin 0.01 mg/ml
-1.8
+0.3
+0.3
+0.2
+0.8
phage
T7_phage 0.00015625 MOI TC4
-0.4
-1.0
+1.2
+0.1
-0.3
stress
M9 Anaerobic 5-Fluorodihydropyrimidine-2,4-dione 5000 uM
+0.5
+0.7
-1.3
+0.1
-0.1
stress
LB Aerobic with Doxycycline hyclate 0.0000015625 mM
-0.6
-0.9
+1.1
+0.4
+0.1
phage
R2_N4_T2_phage10
+0.8
-1.1
-0.2
+0.4
+0.4
stress
LB Aerobic with Tetracycline 0.0000003125 mM
+0.1
-0.8
+0.8
+0.4
+0.7
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-0.4
+1.8
-1.0
+0.6
+0.4
stress
1-ethyl-3-methylimidazolium chloride 80 mM
+0.2
+0.8
+1.7
-0.0
+0.2
carbon source
Ying_Others16 rep A; time point 2
-0.2
+1.4
+4.8
+0.4
+0.2
carbon source
Ying_Others16 rep B; time point 2
+1.0
+1.6
+4.4
+0.2
+0.1
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