Fitness for 5 genes in Escherichia coli BW25113

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500 ntinsD and intQ are separated by 13 nucleotidesintQ and rspB are separated by 187 nucleotidesrspB and rspA are separated by 11 nucleotidesrspA and ynfA are separated by 205 nucleotides b1578: insD - Qin prophage; IS2 insertion element transposase InsAB', C-ter fragment (pseudogene) (RefSeq), at 1,648,869 to 1,649,561 insD b1579: intQ - Qin prophage; predicted defective integrase (RefSeq), at 1,649,575 to 1,650,732 intQ b1580: rspB - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI), at 1,650,920 to 1,651,939 rspB b1581: rspA - predicted dehydratase (NCBI), at 1,651,951 to 1,653,165 rspA b1582: ynfA - hypothetical protein (NCBI), at 1,653,371 to 1,653,697 ynfA
Group Condition b1578 b1579 b1580 b1581 b1582
insD intQ rspB rspA ynfA
carbon source carbon source D-Glucose 0.25mM N.D. -2.0 -1.5 -0.4 -1.4
stress LB Anaerobic with Cephalothin 0.0000078125 mM N.D. -0.1 -0.2 +0.3 -3.2
carbon source carbon source D-Glucose 5mM N.D. -0.3 -1.3 +0.1 -1.6
M9 Glucose 2% aerobic N.D. -2.1 -0.4 -0.0 -0.4
carbon source carbon source D-Mannose 0.5mM N.D. -2.3 -0.6 -0.2 +0.6
nophagecontrol R1_N4_T1_phage0 N.D. -0.8 -0.8 -0.6 -0.3
M9 Acetate 0.5% aerobic N.D. -2.5 -0.5 +0.4 +0.2
M9 Acetate 0.5% aerobic N.D. -2.3 -0.3 +0.6 -0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +0.1 -0.5 -0.9 -1.1
M9 Glucose 2% aerobic N.D. -2.1 -0.3 -0.1 +0.1
M9 Glucose 2% anaerobic N.D. -1.9 -0.2 +0.2 -0.3
phage N4_phage 0.00009375 MOI TC4 N.D. -0.1 +0.7 -1.5 -0.9
predation 6 hr rep1_HD100_10fold_dilution_grewON N.D. -0.8 -0.2 -1.4 +0.8
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. -0.3 -0.3 +0.5 -1.5
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. -0.1 -0.2 +0.6 -1.7
stress Cephalothin 0.01 mg/ml N.D. +0.5 +0.3 -0.3 -1.8
carbon source carbon source D-Mannose 1mM N.D. -1.5 +0.4 +0.3 -0.3
stress LB Anaerobic with Ramoplanin 0.0000625 mM N.D. -1.0 -0.6 +0.7 +0.2
phage R2_N4_T2_phage10 N.D. -0.8 +0.3 -0.7 +0.8
phage N4_phage 0.009375 MOI TC2 N.D. +1.7 -0.0 +0.4 -2.1
stress LB Anaerobic with Spiramycin 0.000125 mM N.D. -0.8 -0.7 +0.5 +1.1
control PBS_cage_1_mouse_1_Cecum_40 hr N.D. -0.7 -0.9 +1.2 +0.5
stress LB Anaerobic with Spiramycin 0.00003125 mM N.D. -0.8 -0.4 +0.6 +0.9
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr N.D. -0.8 -0.4 +1.0 +0.5
stress LB Anaerobic with Spiramycin 0.000125 mM N.D. -0.7 -0.4 +0.4 +1.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +0.2 +0.5 +0.9 -0.9
stress Trimethoprim 0.005 mM N.D. +1.9 +0.4 -2.0 +0.7
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +0.9 +0.6 +0.3 -0.7
stress M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM N.D. +1.0 +0.3 +0.6 +0.6
phage Papa dilution 10-1 cacl2 maltose N.D. +1.4 -0.4 +0.6 +1.3
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