Fitness for 5 genes in Escherichia coli BW25113

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500 ntydfA and ydfB are separated by 1 nucleotidesydfB and ydfC are separated by 29 nucleotidesydfC and dicF are separated by 340 nucleotidesdicF and dicB are separated by 174 nucleotides b1571: ydfA - Qin prophage; predicted protein (NCBI), at 1,646,532 to 1,646,687 ydfA b1572: ydfB - orf, hypothetical protein (VIMSS), at 1,646,689 to 1,646,817 ydfB b1573: ydfC - Qin prophage; predicted protein (RefSeq), at 1,646,847 to 1,647,065 ydfC b1574: dicF - antisense RNA DicF (NCBI), at 1,647,406 to 1,647,458 dicF b1575: dicB - Qin prophage; cell division inhibition protein (NCBI), at 1,647,633 to 1,647,821 dicB
Group Condition b1571 b1572 b1573 b1574 b1575
ydfA ydfB ydfC dicF dicB
predation 6 hr rep2_HD100_10fold_dilution_grewON N.D. +0.6 -2.2 N.D. -5.0
stress Ciprofloxacin Hydrochloride 0.0024 mM N.D. -0.0 -3.0 N.D. -3.5
stress LB Anaerobic with Oxytetracycline 0.00000015625 mM N.D. -0.6 -2.0 N.D. -3.2
motility inner cut, LB soft agar motility assay N.D. -6.1 +0.0 N.D. +0.8
lb LB, Aerobic N.D. +0.1 -3.1 N.D. -2.3
stress LB Anaerobic with Oxytetracycline 0.0000003125 mM N.D. +0.1 -1.5 N.D. -3.2
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. +0.4 -2.8 N.D. -1.9
stress LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM N.D. -1.0 -1.4 N.D. -1.5
motility outer cut, LB soft agar motility assay N.D. -2.5 -1.0 N.D. -0.4
stress LB Anaerobic with Oxytetracycline 0.00000015625 mM N.D. -0.3 -0.3 N.D. -3.3
stress LB Anaerobic with Sulfamonomethoxine 0.00003125 mM N.D. -0.7 -1.0 N.D. -2.1
nophagecontrol T4_T1_phage0 N.D. +0.1 -2.4 N.D. -1.4
stress LB Aerobic with Spiramycin 0.000125 mM N.D. -0.5 +0.2 N.D. -3.4
stress LB Aerobic with Hygromycin B 0.000015625 mM N.D. +0.8 -0.7 N.D. -3.4
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM N.D. +0.6 -0.6 N.D. -3.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. -0.7 -2.7 N.D. +0.4
control PBS_cage_2_mouse_2_Cecum_40 hr N.D. +0.8 +1.8 N.D. -5.5
stress LB Anaerobic with Spiramycin 0.000125 mM N.D. -0.1 +0.6 N.D. -3.3
msc 3hr_mediaonly_rep2_lowinoculum N.D. +0.7 -0.5 N.D. -2.9
control PBS_cage_1_mice _Feces_16 hr N.D. +0.1 +0.6 N.D. -3.2
phage RB68 N.D. -1.2 +1.0 N.D. -2.3
vitamin M9 with Pyridoxine HCl 20 nM and 4-Deoxypyridoxine 0.25 uM N.D. +0.3 +0.6 N.D. -3.0
stress LB Anaerobic with Norfloxacin 0.0000000390625 mM N.D. -0.1 +1.1 N.D. -3.2
nophagecontrol R1_N4_T1_phage0 N.D. -0.3 +1.2 N.D. -2.6
stress LB Aerobic with Benzethonium chloride 0.000015625 mM N.D. +1.2 -1.2 N.D. -1.4
phage Papa dilution 10-1 cacl2 maltose N.D. -1.3 -1.5 N.D. +1.5
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM N.D. +1.3 -1.3 N.D. -1.3
stress LB Aerobic with 8-hydroxy-nitroquinoline 0.00000125 mM N.D. +1.5 -1.7 N.D. -0.8
phage R2_N4_T1_phage10 N.D. -0.4 -2.1 N.D. +2.6
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr N.D. +1.8 +1.2 N.D. -1.1
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