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Fitness for 5 genes in
Escherichia coli BW25113
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
yneF and yneG are separated by 111 nucleotides
yneG and yneH overlap by 1 nucleotides
yneH and b1525 are separated by 63 nucleotides
b1525 and yneJ are separated by 100 nucleotides
b1522: yneF - predicted diguanylate cyclase (NCBI), at 1,608,931 to 1,609,878
yneF
b1523: yneG - hypothetical protein (NCBI), at 1,609,990 to 1,610,349
yneG
b1524: yneH - predicted glutaminase (NCBI), at 1,610,349 to 1,611,275
yneH
b1525: b1525 - putative aldehyde dehydrogenase (VIMSS), at 1,611,339 to 1,612,727
b1525
b1526: yneJ - predicted DNA-binding transcriptional regulator (NCBI), at 1,612,828 to 1,613,709
yneJ
Group
Condition
b1522
b1523
b1524
b1525
b1526
yneF
yneG
yneH
b1525
yneJ
predation
6 hr rep1_HD100_10fold_dilution_grewON
-1.5
-5.0
-5.1
-0.3
-0.8
phage
T7_phage 0.00015625 MOI TC3
-0.8
-3.5
-2.9
-0.8
-0.7
stress
octanol 0.08 vol%
-0.2
-1.3
-5.5
-0.2
-0.6
stress
Trimethoprim 0.005 mM
-2.6
-0.2
-0.6
-1.8
-0.8
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
+0.0
-0.6
-5.0
+0.1
-0.2
phage
Papa dilution 10-1 cacl2 maltose
-1.2
-0.4
-2.8
-0.2
-0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-0.2
-2.6
-1.8
+0.5
-1.1
phage
Phage_N4_cage_3_mouse_3_Cecum_40 hr
-0.4
+0.3
-6.0
+0.9
+0.2
predation
6 hr rep2_HD100_10fold_dilution_grewON
-0.3
-0.0
-5.3
+0.6
+0.1
carbon source
carbon source D-Mannose 0.5mM
-0.5
+0.9
-4.5
-0.3
-0.6
phage
T7_phage 0.0015625 MOI TC2
-1.5
+0.6
-2.4
-0.4
-0.9
phage
R2_N4_T2_phage10
-0.4
-2.0
-2.9
-0.0
+0.9
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.2
+1.8
-4.6
-1.1
-0.4
nophagecontrol
R1_N4_T1_phage0
+0.3
+0.1
-4.0
-0.1
-0.4
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.3
-2.9
-1.4
+0.1
-0.1
carbon source
carbon source D-Glucose 5mM
-0.0
-4.1
+0.3
-0.3
+0.2
control
PBS_cage_1_mouse_2_Cecum_40 hr
-0.2
+0.3
-4.4
+0.1
+0.3
carbon source
carbon source D-Glucose 0.25mM
+0.4
+0.2
-4.7
+0.4
+0.3
phage
T7_phage 0.00015625 MOI TC4
-1.0
-0.0
-3.4
+1.3
+0.4
phage
RB68
+0.0
+0.9
-2.8
+0.0
-0.8
phage
Ste41
-1.1
-3.0
+2.1
-0.6
+0.1
phage
Bas60
+0.5
+0.1
-3.1
+0.2
+0.6
motility
inner cut, LB soft agar motility assay
-0.8
+0.7
-2.6
+0.8
+0.4
phage
R1_N4_T2_phage8
-0.4
+0.3
-2.3
+0.3
+1.2
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.3
-2.3
+1.7
-0.2
+0.4
mzaabcde
mzaABCDE
+0.4
-0.9
+1.7
+0.6
-1.0
retron_ec_48_control
retron_Ec_48_control
+0.7
-1.1
+1.6
+0.4
-0.8
gfp_control
GFP_control
+0.5
-0.9
+1.8
+0.8
-1.2
gfp_control
GFP_control
+0.3
-0.7
+1.9
+0.6
-1.0
mzaabcde
mzaABCDE
+0.9
-0.8
+1.9
+0.8
-0.9
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