Fitness for 5 genes in Escherichia coli BW25113

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500 ntuxaB and yneF are separated by 226 nucleotidesyneF and yneG are separated by 111 nucleotidesyneG and yneH overlap by 1 nucleotidesyneH and b1525 are separated by 63 nucleotides b1521: uxaB - tagaturonate reductase (NCBI), at 1,607,253 to 1,608,704 uxaB b1522: yneF - predicted diguanylate cyclase (NCBI), at 1,608,931 to 1,609,878 yneF b1523: yneG - hypothetical protein (NCBI), at 1,609,990 to 1,610,349 yneG b1524: yneH - predicted glutaminase (NCBI), at 1,610,349 to 1,611,275 yneH b1525: b1525 - putative aldehyde dehydrogenase (VIMSS), at 1,611,339 to 1,612,727 b1525
Group Condition b1521 b1522 b1523 b1524 b1525
uxaB yneF yneG yneH b1525
predation 6 hr rep1_HD100_10fold_dilution_grewON -0.4 -1.5 -5.0 -5.1 -0.3
phage T7_phage 0.00015625 MOI TC3 -0.6 -0.8 -3.5 -2.9 -0.8
stress octanol 0.08 vol% -0.1 -0.2 -1.3 -5.5 -0.2
stress Trimethoprim 0.005 mM -1.1 -2.6 -0.2 -0.6 -1.8
attachment_assay_attached_sample Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB +0.0 +0.0 -0.6 -5.0 +0.1
phage R2_N4_T2_phage10 -0.1 -0.4 -2.0 -2.9 -0.0
phage T7_phage 0.0015625 MOI TC2 -1.5 -1.5 +0.6 -2.4 -0.4
phage Phage_N4_cage_3_mouse_3_Cecum_40 hr +0.2 -0.4 +0.3 -6.0 +0.9
phage Papa dilution 10-1 cacl2 maltose -0.4 -1.2 -0.4 -2.8 -0.2
predation 6 hr rep2_HD100_10fold_dilution_grewON +0.2 -0.3 -0.0 -5.3 +0.6
control PBS_cage_1_mouse_2_Cecum_40 hr -0.3 -0.2 +0.3 -4.4 +0.1
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.6 +0.2 +1.8 -4.6 -1.1
carbon source carbon source D-Glucose 5mM -0.2 -0.0 -4.1 +0.3 -0.3
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM -0.4 +0.3 -2.9 -1.4 +0.1
nophagecontrol R1_N4_T1_phage0 -0.5 +0.3 +0.1 -4.0 -0.1
carbon source carbon source D-Mannose 0.5mM +0.3 -0.5 +0.9 -4.5 -0.3
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 -0.2 -2.6 -1.8 +0.5
phage T7_phage 0.00015625 MOI TC4 -0.7 -1.0 -0.0 -3.4 +1.3
stress Ciprofloxacin Hydrochloride 0.0003 mM +0.1 -0.3 -0.1 -3.4 +0.2
stress 3-methyl-1-butanol 1.25 vol% -0.5 +0.0 -0.7 -2.6 +0.6
stress LB Anaerobic with Trimethoprim 0.00000125 mM +0.2 +0.2 -0.6 -3.2 +0.3
carbon source carbon source D-Glucose 0.25mM +0.5 +0.4 +0.2 -4.7 +0.4
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM +0.3 +0.2 +0.2 -3.3 -0.3
phage R1_N4_T2_phage8 -0.8 -0.4 +0.3 -2.3 +0.3
phage Ste41 +0.3 -1.1 -3.0 +2.1 -0.6
phage Bas60 +0.2 +0.5 +0.1 -3.1 +0.2
motility inner cut, LB soft agar motility assay -0.0 -0.8 +0.7 -2.6 +0.8
control PBS_cage_1_mouse_1_Cecum_40 hr +0.2 -0.3 -2.3 +1.7 -0.2
gfp_control GFP_control +0.2 +0.5 -0.9 +1.8 +0.8
mzaabcde mzaABCDE -0.0 +0.9 -0.8 +1.9 +0.8
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