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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
lsrD and lsrB are separated by 11 nucleotides
lsrB and lsrF are separated by 26 nucleotides
lsrF and lsrG are separated by 23 nucleotides
lsrG and tam are separated by 56 nucleotides
b1515: lsrD - AI2 transporter (NCBI), at 1,602,071 to 1,603,063
lsrD
b1516: lsrB - AI2 transporter (NCBI), at 1,603,075 to 1,604,097
lsrB
b1517: lsrF - hypothetical protein (NCBI), at 1,604,124 to 1,604,999
lsrF
b1518: lsrG - autoinducer-2 (AI-2) modifying protein LsrG (NCBI), at 1,605,023 to 1,605,313
lsrG
b1519: tam - trans-aconitate 2-methyltransferase (NCBI), at 1,605,370 to 1,606,128
tam
Group
Condition
b1515
b1516
b1517
b1518
b1519
lsrD
lsrB
lsrF
lsrG
tam
phage
T7_phage 0.00015625 MOI TC3
-2.4
+0.0
-1.1
+0.3
-2.9
phage
T7_phage 0.0015625 MOI TC2
-0.5
-1.5
-0.3
-2.9
+0.0
phage
R1_N4_T2_phage8
-0.2
-0.7
+0.2
-3.6
-0.4
carbon source
carbon source D-Mannose 1mM
+0.0
+0.4
-0.3
-5.1
+0.5
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.1
-0.5
-0.1
-3.1
-0.2
phage
Ste41
-0.6
-1.0
+0.1
-0.3
-1.1
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.2
+0.2
-0.4
-2.3
-0.2
control
PBS_cage_1_mouse_2_Cecum_40 hr
-0.6
+0.0
+0.3
-2.5
+0.3
stress
3-methyl-1-butanol 1.25 vol%
+0.3
+0.3
-0.0
-3.3
+0.4
stress
Cisplatin 0.1 mg/ml
-0.3
+1.0
-0.2
-2.5
-0.2
phage
Δdbn_10
-0.8
+0.7
-0.3
-2.2
+0.5
phage
N4_phage 0.009375 MOI TC1
+0.0
+0.6
-0.2
-0.9
-1.6
attachment_assay_attached_sample
Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-0.1
-0.5
+0.5
-1.0
-1.0
stress
LB Anaerobic with Phenazine ethosulfate 0.000125 mM
-0.3
+0.8
-0.2
-2.4
+0.1
control
PBS_cage_1_mice _Feces_16 hr
-0.4
-1.6
+0.1
-1.1
+1.0
lb
LB, Aerobic
-0.2
+0.2
-0.3
-2.2
+0.5
nophagecontrol
R1_N4_T1_phage0
-0.3
+0.2
+0.1
-2.1
+0.2
carbon source
acetate (C)
-0.5
+0.2
-1.3
+0.4
-0.5
stress
LB Aerobic with Zinc Pyrithione 0.0000025 mM
+0.3
+0.0
-0.1
-2.1
+0.5
stress
Ciprofloxacin Hydrochloride 0.0024 mM
+0.4
-1.2
-0.2
-0.9
+0.6
stress
Trimethoprim 0.005 mM
+0.3
-1.9
+0.4
+1.7
-1.7
carbon source
carbon source D-Glucose 0.25mM
+0.9
+0.5
-0.1
+0.1
-2.2
control
PBS_cage_1_mice _Feces_22 hr
-0.2
+0.1
+0.3
-1.6
+1.1
phage
N4_phage 0.009375 MOI TC2
+0.4
-0.1
+0.4
-1.9
+0.9
phage
N4_phage 0.0009375 MOI TC2
-0.8
-0.9
+0.2
+0.6
+0.5
phage
T7_phage 0.00015625 MOI TC4
-0.7
-1.6
+1.3
+0.8
-0.1
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.9
-0.6
-0.8
+0.5
+0.3
predation
6 hr rep2_HD100_10fold_dilution_grewON
+0.6
+0.2
-0.1
+1.4
-1.3
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
+0.4
+0.6
-0.1
+1.7
+0.4
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.2
+1.0
-0.1
+1.1
+0.8
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