Fitness for 5 genes in Escherichia coli BW25113

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500 ntlsrD and lsrB are separated by 11 nucleotideslsrB and lsrF are separated by 26 nucleotideslsrF and lsrG are separated by 23 nucleotideslsrG and tam are separated by 56 nucleotides b1515: lsrD - AI2 transporter (NCBI), at 1,602,071 to 1,603,063 lsrD b1516: lsrB - AI2 transporter (NCBI), at 1,603,075 to 1,604,097 lsrB b1517: lsrF - hypothetical protein (NCBI), at 1,604,124 to 1,604,999 lsrF b1518: lsrG - autoinducer-2 (AI-2) modifying protein LsrG (NCBI), at 1,605,023 to 1,605,313 lsrG b1519: tam - trans-aconitate 2-methyltransferase (NCBI), at 1,605,370 to 1,606,128 tam
Group Condition b1515 b1516 b1517 b1518 b1519
lsrD lsrB lsrF lsrG tam
phage T7_phage 0.00015625 MOI TC3 -2.4 +0.0 -1.1 +0.3 -2.9
phage T7_phage 0.0015625 MOI TC2 -0.5 -1.5 -0.3 -2.9 +0.0
phage R1_N4_T2_phage8 -0.2 -0.7 +0.2 -3.6 -0.4
carbon source carbon source D-Mannose 1mM +0.0 +0.4 -0.3 -5.1 +0.5
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.1 -0.5 -0.1 -3.1 -0.2
phage Ste41 -0.6 -1.0 +0.1 -0.3 -1.1
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.2 +0.2 -0.4 -2.3 -0.2
control PBS_cage_1_mouse_2_Cecum_40 hr -0.6 +0.0 +0.3 -2.5 +0.3
stress 3-methyl-1-butanol 1.25 vol% +0.3 +0.3 -0.0 -3.3 +0.4
stress Cisplatin 0.1 mg/ml -0.3 +1.0 -0.2 -2.5 -0.2
phage Δdbn_10 -0.8 +0.7 -0.3 -2.2 +0.5
phage N4_phage 0.009375 MOI TC1 +0.0 +0.6 -0.2 -0.9 -1.6
attachment_assay_attached_sample Time0_Wash=None; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB -0.1 -0.5 +0.5 -1.0 -1.0
stress LB Anaerobic with Phenazine ethosulfate 0.000125 mM -0.3 +0.8 -0.2 -2.4 +0.1
control PBS_cage_1_mice _Feces_16 hr -0.4 -1.6 +0.1 -1.1 +1.0
lb LB, Aerobic -0.2 +0.2 -0.3 -2.2 +0.5
nophagecontrol R1_N4_T1_phage0 -0.3 +0.2 +0.1 -2.1 +0.2
carbon source acetate (C) -0.5 +0.2 -1.3 +0.4 -0.5
stress LB Aerobic with Zinc Pyrithione 0.0000025 mM +0.3 +0.0 -0.1 -2.1 +0.5
stress Ciprofloxacin Hydrochloride 0.0024 mM +0.4 -1.2 -0.2 -0.9 +0.6
stress Trimethoprim 0.005 mM +0.3 -1.9 +0.4 +1.7 -1.7
carbon source carbon source D-Glucose 0.25mM +0.9 +0.5 -0.1 +0.1 -2.2
control PBS_cage_1_mice _Feces_22 hr -0.2 +0.1 +0.3 -1.6 +1.1
phage N4_phage 0.009375 MOI TC2 +0.4 -0.1 +0.4 -1.9 +0.9
phage N4_phage 0.0009375 MOI TC2 -0.8 -0.9 +0.2 +0.6 +0.5
phage T7_phage 0.00015625 MOI TC4 -0.7 -1.6 +1.3 +0.8 -0.1
plasmid transformation vcDART vector transformation; liquid outgrowth assay +0.9 -0.6 -0.8 +0.5 +0.3
predation 6 hr rep2_HD100_10fold_dilution_grewON +0.6 +0.2 -0.1 +1.4 -1.3
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM +0.4 +0.6 -0.1 +1.7 +0.4
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.2 +1.0 -0.1 +1.1 +0.8
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