Fitness for 5 genes in Escherichia coli BW25113

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500 ntb1497 and b1498 are separated by 51 nucleotidesb1498 and ydeO are separated by 401 nucleotidesydeO and yneN are separated by 74 nucleotidesyneN and YdeP are separated by 247 nucleotides b1497: b1497 - putative enzyme (VIMSS), at 1,577,657 to 1,578,814 b1497 b1498: b1498 - putative sulfatase (VIMSS), at 1,578,866 to 1,580,548 b1498 b1499: ydeO - predicted DNA-binding transcriptional acfivator (NCBI), at 1,580,950 to 1,581,711 ydeO b1500: yneN - Two component system connector membrane protein, EvgSA to PhoQP (RefSeq), at 1,581,786 to 1,581,983 yneN b1501: YdeP - putative oxidoreductase, major subunit, acid resistance protein (Katherine Huang), at 1,582,231 to 1,584,510 YdeP
Group Condition b1497 b1498 b1499 b1500 b1501
b1497 b1498 ydeO yneN YdeP
motility outer cut, LB soft agar motility assay -2.4 -0.3 -4.3 N.D. +0.1
M9 Glucose 2% anaerobic -2.9 -0.9 -2.7 N.D. -0.1
M9 Glucose 2% aerobic -2.8 -0.9 -2.5 N.D. -0.1
motility inner cut, LB soft agar motility assay -1.1 -0.2 -4.8 N.D. -0.1
M9 Glucose 2% anaerobic -3.0 -0.7 -2.1 N.D. -0.1
stress Benzalkonium Chloride 0.004 mg/ml -2.7 -0.7 -2.5 N.D. +0.0
M9 Glucose 2% aerobic -2.7 -0.7 -2.3 N.D. -0.1
M9 Glucose 2%, 20 mM NO3- anaerobic -2.3 -0.8 -2.0 N.D. -0.1
motility outer cut, LB soft agar motility assay -1.4 +0.1 -3.7 N.D. +0.1
stress 1-ethyl-3-methylimidazolium chloride 40 mM -1.6 -0.2 -2.6 N.D. -0.1
stress 1-ethyl-3-methylimidazolium chloride 80 mM -1.5 -0.4 -2.0 N.D. -0.0
stress Nalidixic 0.006 mg/ml -1.3 -0.3 -2.0 N.D. -0.0
stress 1-ethyl-3-methylimidazolium acetate 30 mM -1.3 -0.3 -1.7 N.D. -0.1
motility inner cut, LB soft agar motility assay -2.5 -0.6 -0.5 N.D. +0.3
stress D-Cycloserine 0.1 mg/ml -1.1 -0.2 -2.0 N.D. +0.1
motility inner cut, LB soft agar motility assay -1.4 -0.2 -1.9 N.D. +0.2
stress Cisplatin 0.1 mg/ml -1.8 +0.1 -1.4 N.D. +0.2
motility inner cut, LB soft agar motility assay -1.7 -0.6 -0.8 N.D. +0.3
stress Phosphomycin 0.001 mg/ml -2.0 +0.4 -1.2 N.D. +0.4
plasmid transformation mariner vector transformation; liquid outgrowth assay +1.2 -0.8 +1.2 N.D. -0.5
phage Ste41 +1.3 +0.6 +1.3 N.D. +0.1
phage T7_phage 0.0015625 MOI TC2 +1.8 +0.4 +2.0 N.D. -0.6
stress M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM +1.8 +0.3 +1.8 N.D. -0.1
phage Ste41 +1.5 +0.7 +1.5 N.D. +0.1
stress M9 Aerobic 5'-Deoxy-5-fluorocytidine 500 uM +1.7 +0.8 +1.7 N.D. -0.1
phage N4_phage 0.09375 MOI TC1 +2.0 +0.7 +2.0 N.D. -0.2
stress M9 with Methotrexate 1.98 mM 24 hr +2.3 +0.4 +2.1 N.D. +0.1
phage Papa dilution 10-1 cacl2 maltose +3.0 +0.9 +3.1 N.D. -0.4
stress Trimethoprim 0.005 mM +3.0 +1.6 +3.3 N.D. -1.1
phage N4_phage 0.009375 MOI TC2 +3.1 +0.9 +3.2 N.D. +0.3
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