Fitness for 5 genes in Escherichia coli BW25113

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500 ntddpX and b1489 are separated by 257 nucleotidesb1489 and yddV are separated by 24 nucleotidesyddV and yddW are separated by 363 nucleotidesyddW and gadC are separated by 130 nucleotides b1488: ddpX - D-ala-D-ala dipeptidase, Zn-dependent (NCBI), at 1,560,519 to 1,561,100 ddpX b1489: b1489 - putative enzyme (VIMSS), at 1,561,358 to 1,563,757 b1489 b1490: yddV - predicted diguanylate cyclase (RefSeq), at 1,563,782 to 1,565,164 yddV b1491: yddW - predicted liprotein (NCBI), at 1,565,528 to 1,566,847 yddW b1492: gadC - predicted glutamate:gamma-aminobutyric acid antiporter (NCBI), at 1,566,978 to 1,568,513 gadC
Group Condition b1488 b1489 b1490 b1491 b1492
ddpX b1489 yddV yddW gadC
carbon source carbon source D-Mannose 1mM -0.2 -1.2 -2.0 -0.1 -0.1
carbon source Ying_sugar7 rep C; time point 3 -0.9 -1.0 -0.7 -0.2 -0.0
plasmid transformation mariner vector transformation; liquid outgrowth assay -0.2 -0.3 -1.1 -0.6 -0.6
carbon source carbon source D-Mannose 1mM +0.1 -0.4 -1.1 -1.2 -0.3
carbon source carbon source D-Mannose 5mM +0.3 -1.4 -1.4 -0.2 +0.3
carbon source Ying_Nucleobase14 rep C; time point 3 -0.6 -1.1 -0.6 -0.1 +0.0
control PBS_cage_1_mouse_1_Cecum_40 hr -0.6 +0.3 -1.0 -1.1 -0.0
carbon source LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C) -0.4 -1.0 -0.3 -0.5 -0.2
carbon source Ying_Nucleobase14 rep B; time point 2 -0.4 -1.0 -1.0 +0.1 -0.1
carbon source Ying_Nucleobase14 rep C; time point 2 -0.8 -0.7 -0.6 -0.1 -0.1
carbon source LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C) -0.6 -1.0 -0.4 -0.1 -0.2
carbon source Ying_Nucleobase14 rep B; time point 3 -0.2 -1.1 -0.8 -0.3 +0.1
carbon source carbon source D-Glucose 0.25mM +0.4 +0.1 -2.4 -0.3 -0.0
motility inner cut, LB soft agar motility assay -1.0 +0.1 -0.5 -0.5 -0.3
carbon source Ying_sugar7 rep B; time point 3 -0.8 -0.8 -0.5 -0.1 +0.2
control PBS_cage_1_mice _Feces_22 hr +0.5 +0.1 -1.2 -0.9 -0.5
gfp_control GFP_control -2.3 +0.3 +0.0 +0.2 +0.0
carbon source carbon source D-Mannose 0.5mM +0.3 -1.6 -0.3 -0.2 +0.2
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +0.6 -0.7 +0.1 -1.3 -0.1
stress Cisplatin 0.1 mg/ml -1.2 +0.2 -0.3 +0.6 -0.8
control PBS_cage_1_mice _Feces_40 hr +0.5 -0.1 -0.8 -0.6 -0.5
mzaabcde mzaABCDE -1.8 +0.0 -0.0 +0.3 +0.2
control PBS_cage_1_mice _Feces_16 hr +0.4 -0.1 -0.5 -1.0 -0.2
phage T7_phage 0.0015625 MOI TC2 -0.7 -1.4 +0.6 +0.3 -0.0
drt_type_3 DRT_type_3 -1.5 +0.5 +0.2 +0.1 +0.1
phage T7_phage 0.00015625 MOI TC3 -0.9 -0.3 +0.4 +0.4 +0.9
stress LB Anaerobic with Cefoxitin 0.000003125 mM -0.4 -0.2 -0.0 +1.5 -0.2
stress LB Anaerobic with Cefoxitin 0.000003125 mM +0.1 -0.4 +0.2 +1.5 -0.0
stress Trimethoprim 0.005 mM +0.7 -0.1 +1.3 -0.4 -0.1
phage T7_phage 0.00015625 MOI TC4 +0.4 -0.3 +1.2 +1.0 +0.2
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