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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ddpX and b1489 are separated by 257 nucleotides
b1489 and yddV are separated by 24 nucleotides
yddV and yddW are separated by 363 nucleotides
yddW and gadC are separated by 130 nucleotides
b1488: ddpX - D-ala-D-ala dipeptidase, Zn-dependent (NCBI), at 1,560,519 to 1,561,100
ddpX
b1489: b1489 - putative enzyme (VIMSS), at 1,561,358 to 1,563,757
b1489
b1490: yddV - predicted diguanylate cyclase (RefSeq), at 1,563,782 to 1,565,164
yddV
b1491: yddW - predicted liprotein (NCBI), at 1,565,528 to 1,566,847
yddW
b1492: gadC - predicted glutamate:gamma-aminobutyric acid antiporter (NCBI), at 1,566,978 to 1,568,513
gadC
Group
Condition
b1488
b1489
b1490
b1491
b1492
ddpX
b1489
yddV
yddW
gadC
carbon source
carbon source D-Mannose 1mM
-0.2
-1.2
-2.0
-0.1
-0.1
carbon source
Ying_sugar7 rep C; time point 3
-0.9
-1.0
-0.7
-0.2
-0.0
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-0.2
-0.3
-1.1
-0.6
-0.6
carbon source
carbon source D-Mannose 1mM
+0.1
-0.4
-1.1
-1.2
-0.3
carbon source
carbon source D-Mannose 5mM
+0.3
-1.4
-1.4
-0.2
+0.3
carbon source
Ying_Nucleobase14 rep C; time point 3
-0.6
-1.1
-0.6
-0.1
+0.0
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.6
+0.3
-1.0
-1.1
-0.0
carbon source
LMW_DNA_from_Pseudomonas_EB271-B4-1 12 ppm (C)
-0.4
-1.0
-0.3
-0.5
-0.2
carbon source
Ying_Nucleobase14 rep B; time point 2
-0.4
-1.0
-1.0
+0.1
-0.1
carbon source
Ying_Nucleobase14 rep C; time point 2
-0.8
-0.7
-0.6
-0.1
-0.1
carbon source
LMW_DNA_from_Lysinibacillus_FW301-JW8 7.7 ppm (C)
-0.6
-1.0
-0.4
-0.1
-0.2
carbon source
Ying_Nucleobase14 rep B; time point 3
-0.2
-1.1
-0.8
-0.3
+0.1
carbon source
carbon source D-Glucose 0.25mM
+0.4
+0.1
-2.4
-0.3
-0.0
motility
inner cut, LB soft agar motility assay
-1.0
+0.1
-0.5
-0.5
-0.3
carbon source
Ying_sugar7 rep B; time point 3
-0.8
-0.8
-0.5
-0.1
+0.2
control
PBS_cage_1_mice _Feces_22 hr
+0.5
+0.1
-1.2
-0.9
-0.5
gfp_control
GFP_control
-2.3
+0.3
+0.0
+0.2
+0.0
carbon source
carbon source D-Mannose 0.5mM
+0.3
-1.6
-0.3
-0.2
+0.2
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.6
-0.7
+0.1
-1.3
-0.1
stress
Cisplatin 0.1 mg/ml
-1.2
+0.2
-0.3
+0.6
-0.8
control
PBS_cage_1_mice _Feces_40 hr
+0.5
-0.1
-0.8
-0.6
-0.5
mzaabcde
mzaABCDE
-1.8
+0.0
-0.0
+0.3
+0.2
control
PBS_cage_1_mice _Feces_16 hr
+0.4
-0.1
-0.5
-1.0
-0.2
phage
T7_phage 0.0015625 MOI TC2
-0.7
-1.4
+0.6
+0.3
-0.0
drt_type_3
DRT_type_3
-1.5
+0.5
+0.2
+0.1
+0.1
phage
T7_phage 0.00015625 MOI TC3
-0.9
-0.3
+0.4
+0.4
+0.9
stress
LB Anaerobic with Cefoxitin 0.000003125 mM
-0.4
-0.2
-0.0
+1.5
-0.2
stress
LB Anaerobic with Cefoxitin 0.000003125 mM
+0.1
-0.4
+0.2
+1.5
-0.0
stress
Trimethoprim 0.005 mM
+0.7
-0.1
+1.3
-0.4
-0.1
phage
T7_phage 0.00015625 MOI TC4
+0.4
-0.3
+1.2
+1.0
+0.2
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