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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
osmC and ddpF are separated by 55 nucleotides
ddpF and ddpD overlap by 8 nucleotides
ddpD and ddpC overlap by 4 nucleotides
ddpC and ddpB overlap by 4 nucleotides
b1482: osmC - osmotically inducible, stress-inducible membrane protein (NCBI), at 1,554,649 to 1,555,080
osmC
b1483: ddpF - D-ala-D-ala transporter subunit (NCBI), at 1,555,136 to 1,556,062
ddpF
b1484: ddpD - D-ala-D-ala transporter subunit (NCBI), at 1,556,055 to 1,557,041
ddpD
b1485: ddpC - D-ala-D-ala transporter subunit (NCBI), at 1,557,038 to 1,557,934
ddpC
b1486: ddpB - D-ala-D-ala transporter subunit (NCBI), at 1,557,931 to 1,558,953
ddpB
Group
Condition
b1482
b1483
b1484
b1485
b1486
osmC
ddpF
ddpD
ddpC
ddpB
phage
T7_phage 0.0015625 MOI TC2
-2.1
-0.2
+0.0
-4.5
-2.7
motility
outer cut, LB soft agar motility assay
+0.0
+0.1
-0.4
+0.3
-6.0
nophagecontrol
R1_N4_T1_phage0
-5.0
-0.7
+0.3
-0.4
+0.3
stress
LB Anaerobic with Norfloxacin 0.0000000390625 mM
-4.0
-0.1
+0.2
-0.5
-0.3
phage
N4_phage 0.00009375 MOI TC5
-2.6
-0.1
-0.2
-0.2
-1.4
stress
LB Anaerobic with Furaltadone 0.000015625 mM
-2.9
-0.4
+0.2
-0.9
+0.2
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-2.5
-0.0
-0.1
-0.8
+0.0
motility
outer cut, LB soft agar motility assay
+0.2
+0.1
-0.4
-1.5
-1.6
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
-2.5
+0.1
-0.6
+0.0
-0.3
predation
6 hr rep2_HD100_10fold_dilution_grewON
-2.6
-0.2
-0.1
-1.0
+0.8
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-2.0
-0.1
+0.3
-0.7
-0.7
stress
LB Aerobic with Spiramycin 0.000125 mM
-3.2
+0.0
-0.1
+0.1
+0.3
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-2.0
+0.0
+0.2
-0.3
-0.8
phage
N4_phage 0.0009375 MOI TC2
-1.3
+0.2
-0.2
-1.1
-0.6
phage
N4_phage 0.00009375 MOI TC3
-1.7
+0.2
-0.0
-1.1
-0.1
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
-1.4
-0.3
+0.6
-1.7
+0.1
stress
LB Anaerobic with Oxytetracycline 0.00000015625 mM
-2.7
+0.0
+0.4
-0.1
-0.1
mzaabcde
mzaABCDE
-1.9
+0.0
+0.2
+0.3
-1.2
phage
N4_phage 0.00009375 MOI TC4
-1.1
+0.2
-0.5
-1.6
+0.6
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-2.4
-0.1
-0.2
-0.3
+0.5
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-1.7
-0.2
+0.8
-0.7
-0.4
phage
T7_phage 0.00015625 MOI TC3
+0.9
-0.0
-0.5
-0.9
-1.6
stress
LB Anaerobic with Phenazine ethosulfate 0.000125 mM
-2.4
+0.4
+0.4
-0.3
+0.1
stress
LB Aerobic with Trimethoprim 0.00000125 mM
-2.4
-0.2
+0.2
-0.0
+0.6
sir2-hera
SIR2-HerA
-0.8
+0.0
+0.4
+0.5
-1.6
phage
Lambda_ci1857
-1.1
+0.2
+0.3
+0.4
-1.3
stress
LB Anaerobic with Spiramycin 0.000125 mM
-2.1
+0.2
+0.3
-0.4
+0.5
phage
Δdbn_10
-2.1
-0.1
-0.4
+0.6
+0.6
stress
LB Anaerobic with Levofloxacin 0.0000000625 mM
-1.5
+0.1
+0.4
-0.7
+0.6
stress
Chlorite 1.25 mM
+1.1
+0.1
-0.3
-0.1
-1.5
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