Fitness for 5 genes in Escherichia coli BW25113

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500 ntydcI and ydcJ are separated by 216 nucleotidesydcJ and mdoD are separated by 224 nucleotidesmdoD and ydcH are separated by 139 nucleotidesydcH and rimL are separated by 62 nucleotides b1422: ydcI - putative transcriptional regulator LYSR-type (VIMSS), at 1,492,172 to 1,493,095 ydcI b1423: ydcJ - hypothetical protein (NCBI), at 1,493,312 to 1,494,655 ydcJ b1424: mdoD - glucan biosynthesis protein, periplasmic (RefSeq), at 1,494,880 to 1,496,535 mdoD b1426: ydcH - orf, hypothetical protein (VIMSS), at 1,496,675 to 1,496,899 ydcH b1427: rimL - ribosomal-protein-L7/L12-serine acetyltransferase (NCBI), at 1,496,962 to 1,497,501 rimL
Group Condition b1422 b1423 b1424 b1426 b1427
ydcI ydcJ mdoD ydcH rimL
carbon source carbon source D-Mannose 1mM +0.0 +0.0 -0.1 -6.2 -0.2
phage T7_phage 0.0015625 MOI TC2 -2.0 -0.2 +0.4 -0.1 -3.9
stress LB Aerobic with Phenazine ethosulfate 0.0000625 mM -0.3 -0.6 -0.6 -3.9 +0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay -5.8 +0.3 +0.6 +0.8 +0.1
stress LB Aerobic with Irgasan 0.0000003125 mM +0.2 -0.4 +0.1 -1.8 -1.5
carbon source D-Glucosamine Hydrochloride (C) -0.1 -0.4 -0.3 -3.1 +0.5
carbon source acetate (C) +1.2 -0.6 -0.6 -3.7 +0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay -3.9 +0.3 +0.8 -0.5 +0.5
plasmid transformation mariner vector transformation; liquid outgrowth assay -3.7 +0.8 +0.6 -0.3 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay -4.8 +0.3 +1.1 -0.5 +1.9
carbon source Ying_sugar7 rep C; time point 3 +0.9 -0.8 -0.5 -1.8 +0.5
plasmid transformation vcDART vector transformation; liquid outgrowth assay -2.8 +0.0 +0.2 +0.7 +0.1
carbon source acetate (C) +1.2 -0.6 -0.7 -1.9 +0.6
protonated/deuterated deuterated glucose in H2O +0.6 -0.4 +0.1 -2.4 +0.8
carbon source succinate (C) +1.7 -0.2 -0.9 -1.8 +0.6
carbon source a-Ketoglutaric (C) +3.5 -0.7 -0.6 -2.9 +0.3
plasmid transformation mariner vector transformation; liquid outgrowth assay -2.6 +0.4 +0.5 +0.6 +0.8
carbon source D-Lactate (C) +1.8 -0.6 -0.5 -1.3 +0.3
carbon source a-Ketoglutaric (C) +3.5 -0.9 -0.8 -2.1 +0.2
carbon source L-Malic (C) +2.4 -0.2 -1.3 -1.2 +0.3
carbon source L-Malic (C) +2.5 -0.2 -0.9 -1.5 +0.1
stress LB Aerobic with Ramoplanin 0.000125 mM +0.1 -0.7 +2.1 -0.5 -0.7
control PBS_cage_2_mouse_2_Cecum_40 hr -0.3 -0.6 -0.4 +2.6 -1.0
control PBS_cage_1_mouse_1_Cecum_40 hr -0.5 -0.9 -0.7 +1.4 +1.0
phage T7_phage 0.00015625 MOI TC4 -1.3 +0.8 +0.2 +1.7 -0.3
phage T7_phage 0.0015625 MOI TC1 -0.6 +0.6 +0.4 +1.8 -0.5
carbon source Ying_OrganicAcid7 rep B; time point 3 +2.7 +0.2 -0.2 -0.6 +0.3
stress LB Aerobic with Ramoplanin 0.000125 mM +0.5 -1.1 +2.5 +0.5 +0.2
control PBS_cage_2_mice _Feces_16 hr -0.1 +0.5 -0.6 +3.0 +0.3
stress Bacitracin 1 mg/ml -0.2 +0.3 +3.4 +0.8 +0.0
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