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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ydcI and ydcJ are separated by 216 nucleotides
ydcJ and mdoD are separated by 224 nucleotides
mdoD and ydcH are separated by 139 nucleotides
ydcH and rimL are separated by 62 nucleotides
b1422: ydcI - putative transcriptional regulator LYSR-type (VIMSS), at 1,492,172 to 1,493,095
ydcI
b1423: ydcJ - hypothetical protein (NCBI), at 1,493,312 to 1,494,655
ydcJ
b1424: mdoD - glucan biosynthesis protein, periplasmic (RefSeq), at 1,494,880 to 1,496,535
mdoD
b1426: ydcH - orf, hypothetical protein (VIMSS), at 1,496,675 to 1,496,899
ydcH
b1427: rimL - ribosomal-protein-L7/L12-serine acetyltransferase (NCBI), at 1,496,962 to 1,497,501
rimL
Group
Condition
b1422
b1423
b1424
b1426
b1427
ydcI
ydcJ
mdoD
ydcH
rimL
carbon source
carbon source D-Mannose 1mM
+0.0
+0.0
-0.1
-6.2
-0.2
phage
T7_phage 0.0015625 MOI TC2
-2.0
-0.2
+0.4
-0.1
-3.9
stress
LB Aerobic with Phenazine ethosulfate 0.0000625 mM
-0.3
-0.6
-0.6
-3.9
+0.5
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-5.8
+0.3
+0.6
+0.8
+0.1
stress
LB Aerobic with Irgasan 0.0000003125 mM
+0.2
-0.4
+0.1
-1.8
-1.5
carbon source
D-Glucosamine Hydrochloride (C)
-0.1
-0.4
-0.3
-3.1
+0.5
carbon source
acetate (C)
+1.2
-0.6
-0.6
-3.7
+0.6
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-3.9
+0.3
+0.8
-0.5
+0.5
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-3.7
+0.8
+0.6
-0.3
+0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-4.8
+0.3
+1.1
-0.5
+1.9
carbon source
Ying_sugar7 rep C; time point 3
+0.9
-0.8
-0.5
-1.8
+0.5
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-2.8
+0.0
+0.2
+0.7
+0.1
carbon source
acetate (C)
+1.2
-0.6
-0.7
-1.9
+0.6
protonated/deuterated
deuterated glucose in H2O
+0.6
-0.4
+0.1
-2.4
+0.8
carbon source
succinate (C)
+1.7
-0.2
-0.9
-1.8
+0.6
carbon source
a-Ketoglutaric (C)
+3.5
-0.7
-0.6
-2.9
+0.3
plasmid transformation
mariner vector transformation; liquid outgrowth assay
-2.6
+0.4
+0.5
+0.6
+0.8
carbon source
D-Lactate (C)
+1.8
-0.6
-0.5
-1.3
+0.3
carbon source
a-Ketoglutaric (C)
+3.5
-0.9
-0.8
-2.1
+0.2
carbon source
L-Malic (C)
+2.4
-0.2
-1.3
-1.2
+0.3
carbon source
L-Malic (C)
+2.5
-0.2
-0.9
-1.5
+0.1
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.1
-0.7
+2.1
-0.5
-0.7
control
PBS_cage_2_mouse_2_Cecum_40 hr
-0.3
-0.6
-0.4
+2.6
-1.0
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.5
-0.9
-0.7
+1.4
+1.0
phage
T7_phage 0.00015625 MOI TC4
-1.3
+0.8
+0.2
+1.7
-0.3
phage
T7_phage 0.0015625 MOI TC1
-0.6
+0.6
+0.4
+1.8
-0.5
carbon source
Ying_OrganicAcid7 rep B; time point 3
+2.7
+0.2
-0.2
-0.6
+0.3
stress
LB Aerobic with Ramoplanin 0.000125 mM
+0.5
-1.1
+2.5
+0.5
+0.2
control
PBS_cage_2_mice _Feces_16 hr
-0.1
+0.5
-0.6
+3.0
+0.3
stress
Bacitracin 1 mg/ml
-0.2
+0.3
+3.4
+0.8
+0.0
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