Fitness for 5 genes in Escherichia coli BW25113

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500 ntsokB and trg are separated by 295 nucleotidestrg and ydcI are separated by 37 nucleotidesydcI and ydcJ are separated by 216 nucleotidesydcJ and mdoD are separated by 224 nucleotides b4429: sokB - Antisense sRNA blocking mokB, and hence hokB, translation (RefSeq), at 1,490,143 to 1,490,198 sokB b1421: trg - methyl-accepting chemotaxis protein III, ribose and galactose sensor receptor (NCBI), at 1,490,494 to 1,492,134 trg b1422: ydcI - putative transcriptional regulator LYSR-type (VIMSS), at 1,492,172 to 1,493,095 ydcI b1423: ydcJ - hypothetical protein (NCBI), at 1,493,312 to 1,494,655 ydcJ b1424: mdoD - glucan biosynthesis protein, periplasmic (RefSeq), at 1,494,880 to 1,496,535 mdoD
Group Condition b4429 b1421 b1422 b1423 b1424
sokB trg ydcI ydcJ mdoD
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. -0.1 -5.8 +0.3 +0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. -0.9 -2.8 +0.0 +0.2
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. -0.4 -3.9 +0.3 +0.8
stress Trimethoprim 0.005 mM N.D. -0.2 -0.8 -1.3 -0.9
phage RB68 N.D. -0.5 -1.0 -0.2 -1.0
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. -0.2 -3.7 +0.8 +0.6
plasmid transformation vcDART vector transformation; liquid outgrowth assay N.D. +1.0 -4.8 +0.3 +1.1
phage T7_phage 0.0015625 MOI TC2 N.D. -0.4 -2.0 -0.2 +0.4
phage Δdbn_10 N.D. -0.7 -0.1 -2.2 +0.7
plasmid transformation mariner vector transformation; liquid outgrowth assay N.D. +0.1 -2.6 +0.4 +0.5
carbon source Uridine 0.4095 mM (C) N.D. -0.2 -1.9 +0.6 -0.1
carbon source acetate (C) N.D. +0.2 +1.2 -0.6 -0.7
carbon source casaminos (C) N.D. +0.3 +1.4 -0.6 -0.6
carbon source acetate (C) N.D. +0.6 +1.2 -0.6 -0.6
carbon source D-Lactate (C) N.D. +0.1 +1.8 -0.6 -0.5
carbon source succinate (C) N.D. +0.6 +1.7 -0.2 -0.9
stress LB Aerobic with Novobiocin 0.0000625 mM N.D. -0.1 +0.2 -0.7 +2.0
carbon source Ying_OrganicAcid7 rep A; time point 3 N.D. +0.1 +2.1 -0.6 -0.1
carbon source Acetate (C) N.D. +0.8 +1.6 -0.3 -0.5
carbon source succinate (C) N.D. +0.6 +1.9 -0.1 -0.8
carbon source L-Malic (C) N.D. +0.8 +2.4 -0.2 -1.3
carbon source L-Malic (C) N.D. +0.5 +2.5 -0.2 -0.9
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. +0.5 +0.1 -0.7 +2.1
carbon source Ying_OrganicAcid7 rep C; time point 3 N.D. -0.3 +2.3 +0.2 -0.2
carbon source Acetate (C) N.D. +0.9 +1.6 -0.3 -0.0
stress LB Aerobic with Ramoplanin 0.000125 mM N.D. +0.2 +0.5 -1.1 +2.5
carbon source a-Ketoglutaric (C) N.D. +0.5 +3.5 -0.9 -0.8
carbon source Ying_OrganicAcid7 rep B; time point 3 N.D. +0.2 +2.7 +0.2 -0.2
carbon source a-Ketoglutaric (C) N.D. +0.8 +3.5 -0.7 -0.6
stress Bacitracin 1 mg/ml N.D. +0.2 -0.2 +0.3 +3.4
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