Fitness for 5 genes in Escherichia coli BW25113

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500 ntydbC and ydbD are separated by 62 nucleotidesydbD and b1408 are separated by 170 nucleotidesb1408 and ynbB overlap by 1 nucleotidesynbB and ynbC are separated by 15 nucleotides b1406: ydbC - predicted oxidoreductase, NAD(P)-binding (NCBI), at 1,472,245 to 1,473,105 ydbC b1407: ydbD - orf, hypothetical protein (VIMSS), at 1,473,168 to 1,475,474 ydbD b1408: b1408 - probable enzyme (VIMSS), at 1,475,645 to 1,476,250 b1408 b1409: ynbB - predicted CDP-diglyceride synthase (NCBI), at 1,476,250 to 1,477,146 ynbB b1410: ynbC - predicted hydrolase (NCBI), at 1,477,162 to 1,478,919 ynbC
Group Condition b1406 b1407 b1408 b1409 b1410
ydbC ydbD b1408 ynbB ynbC
M9 Glucose 2% anaerobic -0.0 -1.3 -0.2 -2.3 -0.1
M9 Glucose 2%, 20 mM NO3- anaerobic -0.0 -1.2 -0.2 -2.6 +0.1
M9 Glucose 2% aerobic -0.1 -1.2 -0.2 -2.6 +0.1
motility outer cut, LB soft agar motility assay +0.1 -1.1 -0.7 -1.5 -0.4
M9 Glucose 2% aerobic -0.2 -1.1 -0.2 -2.0 +0.1
stress 1-ethyl-3-methylimidazolium chloride 80 mM -0.4 -0.8 -0.6 -1.3 +0.1
M9 Glucose 2% anaerobic -0.0 -1.0 -0.1 -1.5 +0.0
stress Benzalkonium Chloride 0.004 mg/ml -0.4 -0.7 -0.6 -1.3 +0.4
M9 Acetate 0.5% aerobic +0.2 -1.0 -0.2 -1.5 -0.1
motility outer cut, LB soft agar motility assay -0.1 -0.9 +0.2 -1.4 +0.1
motility inner cut, LB soft agar motility assay +0.2 -0.6 +0.3 -1.5 +0.1
micoeukaryotes Tetrahymena sp., Mixed culture-1 -0.5 +0.2 +0.4 +0.6 -1.1
motility inner cut, LB soft agar motility assay +1.0 -0.8 -0.3 -0.6 +0.4
phage N4_phage 0.00009375 MOI TC4 -1.0 +0.5 -0.3 +0.9 -0.2
plasmid transformation mariner vector transformation; liquid outgrowth assay +0.0 +0.6 -1.4 +1.2 -0.5
carbon source carbon source D-Glucose 0.25mM -1.1 -0.7 +1.3 +1.1 +0.0
phage N4_phage 0.0009375 MOI TC2 -0.3 +0.5 -0.6 +1.0 +0.2
phage Ste41 -0.6 +0.8 +0.5 +1.4 -0.8
phage Δdbn_10 +0.0 +0.5 +0.3 +1.2 -0.6
control PBS_cage_1_mouse_1_Cecum_40 hr -0.4 -0.0 +0.7 +1.0 +0.5
stress Ciprofloxacin Hydrochloride 0.000156 mM -0.4 +0.6 +0.3 +1.5 -0.1
phage Ste41 +0.6 +1.1 -0.5 +1.3 -0.4
phage N4_phage 0.09375 MOI TC1 +0.2 +0.8 -0.2 +1.9 -0.5
phage T7_phage 0.0015625 MOI TC2 +0.1 +0.4 -0.3 +1.4 +0.7
stress Ciprofloxacin Hydrochloride 0.0003 mM +0.4 +1.0 +0.4 +1.0 -0.3
phage RB68 +0.2 +0.6 +0.7 +1.2 -0.1
stress M9 with Methotrexate 1.98 mM 24 hr +0.2 +0.8 +0.0 +2.0 +0.1
phage Papa dilution 10-1 cacl2 maltose -0.3 +1.4 +0.6 +2.9 -0.2
phage N4_phage 0.009375 MOI TC2 -0.1 +1.6 +0.8 +2.8 -0.2
stress Trimethoprim 0.005 mM -0.0 +2.1 +1.0 +3.1 -0.2
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