Fitness for 5 genes in Escherichia coli BW25113

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500 ntydaO and intR are separated by 51 nucleotidesintR and ydaQ are separated by 1 nucleotidesydaQ and ydaC are separated by 78 nucleotidesydaC and lar overlap by 8 nucleotides b1344: ydaO - predicted C32 tRNA thiolase (NCBI), at 1,409,037 to 1,409,972 ydaO b1345: intR - Rac prophage; integrase (NCBI), at 1,410,024 to 1,411,259 intR b1346: ydaQ - orf, hypothetical protein (VIMSS), at 1,411,261 to 1,411,476 ydaQ b1347: ydaC - Rac prophage; predicted protein (NCBI), at 1,411,555 to 1,411,764 ydaC b1348: lar - Rac prophage; restriction alleviation protein (NCBI), at 1,411,757 to 1,411,951 lar
Group Condition b1344 b1345 b1346 b1347 b1348
ydaO intR ydaQ ydaC lar
control PBS_cage_1_mouse_1_Cecum_40 hr -0.3 +0.0 -5.1 +0.3 -5.8
motility inner cut, LB soft agar motility assay +1.0 +0.3 -1.8 -6.0 -0.9
msc 3hr_MSC_rep1_lowinoculum -1.0 -0.0 -5.3 -0.1 -0.6
carbon source carbon source D-Mannose 0.5mM +0.5 -0.3 -5.8 -1.6 +0.7
phage R2_N4_T1_phage10 -0.4 -0.2 -4.0 -0.2 -1.2
msc 3hr_mediaonly_rep2_lowinoculum +0.6 -0.2 -4.7 +0.1 -1.7
stress LB Anaerobic with Nitrofurantoin 0.000015625 mM -0.8 -0.2 -2.6 -0.3 -1.4
stress LB Aerobic with Tetracycline 0.0000003125 mM -0.6 +0.0 -3.3 -0.6 -0.7
stress LB Anaerobic with Ticarcillin 0.00003125 mM -0.4 -0.2 -2.7 -0.5 -1.1
stress LB Aerobic with Hygromycin B 0.000015625 mM -0.8 +0.2 -2.5 -0.6 -1.2
motility outer cut, LB soft agar motility assay -0.6 -0.3 -1.0 -2.3 -0.6
stress LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM +0.2 -0.1 -2.9 -0.3 -1.6
lb LB, Aerobic +0.2 +0.1 -3.3 -0.6 -0.5
predation 18 hr rep1_Tiberius_10fold_dilution +0.4 +0.1 -2.7 -0.6 -1.0
phage R2_N4_T2_phage10 +0.6 -0.3 -4.1 -0.2 +0.0
nophagecontrol R1_N4_T1_phage0 +0.5 -0.7 -3.1 -0.4 +0.0
stress Ciprofloxacin Hydrochloride 0.0003 mM -0.0 -0.2 -2.6 +1.2 -1.9
carbon source Glycolic Acid (C) +0.7 -0.5 -1.2 -2.2 +0.2
carbon source carbon source D-Glucose 0.25mM +1.0 -0.2 -2.2 -1.3 +0.7
stress Cisplatin 0.1 mg/ml -0.9 -0.4 +1.0 -1.2 +1.1
phage Ste41 +0.8 +0.2 -1.5 +1.6 -1.2
phage N4_phage 0.009375 MOI TC1 +0.9 +0.2 -2.2 +1.4 +0.5
phage Δdbn_10 -0.7 +0.2 +1.5 +1.6 -1.6
phage T7_phage 0.00015625 MOI TC3 +0.7 +0.5 -3.3 +0.5 +2.6
stress octanol 0.08 vol% +1.5 -0.2 +0.1 +1.9 -2.1
phage Ste41 -0.5 +0.7 +1.7 +1.5 -0.4
phage N4_phage 0.009375 MOI TC2 +0.8 -0.7 -0.7 +3.1 +1.1
stress Trimethoprim 0.005 mM -0.3 -0.0 +1.0 +3.2 +0.6
micoeukaryotes Tetrahymena sp., Mixed culture-1 +0.8 -0.1 +1.9 +1.2 +1.0
phage T7_phage 0.00015625 MOI TC4 +1.0 +0.6 +1.9 +1.2 +0.9
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