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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ydaO and intR are separated by 51 nucleotides
intR and ydaQ are separated by 1 nucleotides
ydaQ and ydaC are separated by 78 nucleotides
ydaC and lar overlap by 8 nucleotides
b1344: ydaO - predicted C32 tRNA thiolase (NCBI), at 1,409,037 to 1,409,972
ydaO
b1345: intR - Rac prophage; integrase (NCBI), at 1,410,024 to 1,411,259
intR
b1346: ydaQ - orf, hypothetical protein (VIMSS), at 1,411,261 to 1,411,476
ydaQ
b1347: ydaC - Rac prophage; predicted protein (NCBI), at 1,411,555 to 1,411,764
ydaC
b1348: lar - Rac prophage; restriction alleviation protein (NCBI), at 1,411,757 to 1,411,951
lar
Group
Condition
b1344
b1345
b1346
b1347
b1348
ydaO
intR
ydaQ
ydaC
lar
control
PBS_cage_1_mouse_1_Cecum_40 hr
-0.3
+0.0
-5.1
+0.3
-5.8
motility
inner cut, LB soft agar motility assay
+1.0
+0.3
-1.8
-6.0
-0.9
msc
3hr_MSC_rep1_lowinoculum
-1.0
-0.0
-5.3
-0.1
-0.6
carbon source
carbon source D-Mannose 0.5mM
+0.5
-0.3
-5.8
-1.6
+0.7
phage
R2_N4_T1_phage10
-0.4
-0.2
-4.0
-0.2
-1.2
msc
3hr_mediaonly_rep2_lowinoculum
+0.6
-0.2
-4.7
+0.1
-1.7
stress
LB Anaerobic with Nitrofurantoin 0.000015625 mM
-0.8
-0.2
-2.6
-0.3
-1.4
stress
LB Aerobic with Tetracycline 0.0000003125 mM
-0.6
+0.0
-3.3
-0.6
-0.7
stress
LB Anaerobic with Ticarcillin 0.00003125 mM
-0.4
-0.2
-2.7
-0.5
-1.1
stress
LB Aerobic with Hygromycin B 0.000015625 mM
-0.8
+0.2
-2.5
-0.6
-1.2
motility
outer cut, LB soft agar motility assay
-0.6
-0.3
-1.0
-2.3
-0.6
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.2
-0.1
-2.9
-0.3
-1.6
lb
LB, Aerobic
+0.2
+0.1
-3.3
-0.6
-0.5
predation
18 hr rep1_Tiberius_10fold_dilution
+0.4
+0.1
-2.7
-0.6
-1.0
phage
R2_N4_T2_phage10
+0.6
-0.3
-4.1
-0.2
+0.0
nophagecontrol
R1_N4_T1_phage0
+0.5
-0.7
-3.1
-0.4
+0.0
stress
Ciprofloxacin Hydrochloride 0.0003 mM
-0.0
-0.2
-2.6
+1.2
-1.9
carbon source
Glycolic Acid (C)
+0.7
-0.5
-1.2
-2.2
+0.2
carbon source
carbon source D-Glucose 0.25mM
+1.0
-0.2
-2.2
-1.3
+0.7
stress
Cisplatin 0.1 mg/ml
-0.9
-0.4
+1.0
-1.2
+1.1
phage
Ste41
+0.8
+0.2
-1.5
+1.6
-1.2
phage
N4_phage 0.009375 MOI TC1
+0.9
+0.2
-2.2
+1.4
+0.5
phage
Δdbn_10
-0.7
+0.2
+1.5
+1.6
-1.6
phage
T7_phage 0.00015625 MOI TC3
+0.7
+0.5
-3.3
+0.5
+2.6
stress
octanol 0.08 vol%
+1.5
-0.2
+0.1
+1.9
-2.1
phage
Ste41
-0.5
+0.7
+1.7
+1.5
-0.4
phage
N4_phage 0.009375 MOI TC2
+0.8
-0.7
-0.7
+3.1
+1.1
stress
Trimethoprim 0.005 mM
-0.3
-0.0
+1.0
+3.2
+0.6
micoeukaryotes
Tetrahymena sp., Mixed culture-1
+0.8
-0.1
+1.9
+1.2
+1.0
phage
T7_phage 0.00015625 MOI TC4
+1.0
+0.6
+1.9
+1.2
+0.9
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