Fitness for 5 genes in Escherichia coli BW25113

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500 ntfnrS and dbpA are separated by 260 nucleotidesdbpA and ydaO are separated by 128 nucleotidesydaO and intR are separated by 51 nucleotidesintR and ydaQ are separated by 1 nucleotides b4699: fnrS - FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq), at 1,407,153 to 1,407,274 fnrS b1343: dbpA - ATP-dependent RNA helicase, specific for 23S rRNA (NCBI), at 1,407,535 to 1,408,908 dbpA b1344: ydaO - predicted C32 tRNA thiolase (NCBI), at 1,409,037 to 1,409,972 ydaO b1345: intR - Rac prophage; integrase (NCBI), at 1,410,024 to 1,411,259 intR b1346: ydaQ - orf, hypothetical protein (VIMSS), at 1,411,261 to 1,411,476 ydaQ
Group Condition b4699 b1343 b1344 b1345 b1346
fnrS dbpA ydaO intR ydaQ
phage Phage_N4_cage_3_mouse_4_Cecum_40 hr -5.4 -0.4 +0.7 -0.4 -2.9
msc 3hr_MSC_rep1_lowinoculum -0.9 +0.4 -1.0 -0.0 -5.3
carbon source carbon source D-Glucose 1mM -5.0 -0.2 -0.7 -0.2 -0.3
carbon source carbon source D-Mannose 1mM -4.8 +0.0 +0.2 +0.2 -1.6
stress LB Aerobic with Ramoplanin 0.000125 mM -2.9 +0.6 -2.6 +0.2 -1.0
sir2-hera SIR2-HerA -5.1 -0.0 +0.5 +0.1 -1.1
motility outer cut, LB soft agar motility assay -2.9 -0.3 -0.6 -0.3 -1.0
phage Ste41 -3.2 -1.1 +0.8 +0.2 -1.5
stress LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM -2.4 +0.2 -0.5 -0.3 -1.8
carbon source carbon source D-Glucose 1mM -4.9 -0.0 +0.2 -0.2 +0.3
msc 3hr_mediaonly_rep2_lowinoculum -0.4 +0.2 +0.6 -0.2 -4.7
carbon source carbon source D-Mannose 0.5mM -4.7 -0.5 -0.5 +0.6 +0.7
stress LB Anaerobic with Nitrofurantoin 0.00003125 mM -2.2 +0.5 -0.6 -0.1 -2.1
carbon source carbon source D-Mannose 0.5mM +1.3 +0.3 +0.5 -0.3 -5.8
stress LB Anaerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM -2.5 +0.6 -0.5 -0.2 -1.4
carbon source carbon source D-Glucose 0.25mM -4.8 +0.6 +1.3 +0.1 -0.4
carbon source carbon source D-Glucose 0.25mM +0.1 -1.9 +1.0 -0.2 -2.2
control PBS_cage_1_mouse_1_Cecum_40 hr +2.9 -0.4 -0.3 +0.0 -5.1
stress octanol 0.08 vol% -3.0 -0.9 +1.5 -0.2 +0.1
phage N4_phage 0.00009375 MOI TC4 -2.8 +0.5 -0.9 -0.1 +1.0
phage R2_N4_T2_phage10 +1.2 +0.2 +0.6 -0.3 -4.1
phage Δdbn_10 -3.1 -0.1 -0.7 +0.2 +1.5
phage R2_N4_T1_phage10 +2.2 +0.2 -0.4 -0.2 -4.0
phage T7_phage 0.0015625 MOI TC2 -2.6 -0.9 +0.8 +0.7 -0.1
stress LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM +1.6 -0.2 -1.2 -0.4 -1.7
plasmid transformation vcDART vector transformation; liquid outgrowth assay -3.6 +1.8 -0.6 -0.3 +2.1
predation 6 hr rep2_HD100_10fold_dilution_grewON +1.3 -2.4 -0.2 -0.1 +1.0
phage T7_phage 0.00015625 MOI TC3 +0.8 +1.4 +0.7 +0.5 -3.3
phage T7_phage 0.00015625 MOI TC4 -1.4 -0.7 +1.0 +0.6 +1.9
stress LB Anaerobic with Benzethonium chloride 0.00003125 mM +3.9 +1.3 -1.4 -0.2 -0.7
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