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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
fnrS and dbpA are separated by 260 nucleotides
dbpA and ydaO are separated by 128 nucleotides
ydaO and intR are separated by 51 nucleotides
intR and ydaQ are separated by 1 nucleotides
b4699: fnrS - FNR-activated anaerobic sRNA antisense regulator down regulates cydDC, metE, sodA, sodB, Hfq-dependent; mediates negative FNR regulation (RefSeq), at 1,407,153 to 1,407,274
fnrS
b1343: dbpA - ATP-dependent RNA helicase, specific for 23S rRNA (NCBI), at 1,407,535 to 1,408,908
dbpA
b1344: ydaO - predicted C32 tRNA thiolase (NCBI), at 1,409,037 to 1,409,972
ydaO
b1345: intR - Rac prophage; integrase (NCBI), at 1,410,024 to 1,411,259
intR
b1346: ydaQ - orf, hypothetical protein (VIMSS), at 1,411,261 to 1,411,476
ydaQ
Group
Condition
b4699
b1343
b1344
b1345
b1346
fnrS
dbpA
ydaO
intR
ydaQ
phage
Phage_N4_cage_3_mouse_4_Cecum_40 hr
-5.4
-0.4
+0.7
-0.4
-2.9
msc
3hr_MSC_rep1_lowinoculum
-0.9
+0.4
-1.0
-0.0
-5.3
carbon source
carbon source D-Glucose 1mM
-5.0
-0.2
-0.7
-0.2
-0.3
carbon source
carbon source D-Mannose 1mM
-4.8
+0.0
+0.2
+0.2
-1.6
stress
LB Aerobic with Ramoplanin 0.000125 mM
-2.9
+0.6
-2.6
+0.2
-1.0
sir2-hera
SIR2-HerA
-5.1
-0.0
+0.5
+0.1
-1.1
motility
outer cut, LB soft agar motility assay
-2.9
-0.3
-0.6
-0.3
-1.0
phage
Ste41
-3.2
-1.1
+0.8
+0.2
-1.5
stress
LB Anaerobic with 8-hydroxy-nitroquinoline 0.0000025 mM
-2.4
+0.2
-0.5
-0.3
-1.8
carbon source
carbon source D-Glucose 1mM
-4.9
-0.0
+0.2
-0.2
+0.3
msc
3hr_mediaonly_rep2_lowinoculum
-0.4
+0.2
+0.6
-0.2
-4.7
carbon source
carbon source D-Mannose 0.5mM
-4.7
-0.5
-0.5
+0.6
+0.7
stress
LB Anaerobic with Nitrofurantoin 0.00003125 mM
-2.2
+0.5
-0.6
-0.1
-2.1
carbon source
carbon source D-Mannose 0.5mM
+1.3
+0.3
+0.5
-0.3
-5.8
stress
LB Anaerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
-2.5
+0.6
-0.5
-0.2
-1.4
carbon source
carbon source D-Glucose 0.25mM
-4.8
+0.6
+1.3
+0.1
-0.4
carbon source
carbon source D-Glucose 0.25mM
+0.1
-1.9
+1.0
-0.2
-2.2
control
PBS_cage_1_mouse_1_Cecum_40 hr
+2.9
-0.4
-0.3
+0.0
-5.1
stress
octanol 0.08 vol%
-3.0
-0.9
+1.5
-0.2
+0.1
phage
N4_phage 0.00009375 MOI TC4
-2.8
+0.5
-0.9
-0.1
+1.0
phage
R2_N4_T2_phage10
+1.2
+0.2
+0.6
-0.3
-4.1
phage
Δdbn_10
-3.1
-0.1
-0.7
+0.2
+1.5
phage
R2_N4_T1_phage10
+2.2
+0.2
-0.4
-0.2
-4.0
phage
T7_phage 0.0015625 MOI TC2
-2.6
-0.9
+0.8
+0.7
-0.1
stress
LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM
+1.6
-0.2
-1.2
-0.4
-1.7
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-3.6
+1.8
-0.6
-0.3
+2.1
predation
6 hr rep2_HD100_10fold_dilution_grewON
+1.3
-2.4
-0.2
-0.1
+1.0
phage
T7_phage 0.00015625 MOI TC3
+0.8
+1.4
+0.7
+0.5
-3.3
phage
T7_phage 0.00015625 MOI TC4
-1.4
-0.7
+1.0
+0.6
+1.9
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+3.9
+1.3
-1.4
-0.2
-0.7
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