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Protein
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Fitness for 5 genes in
Escherichia coli BW25113
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500 nt
ydaH and abgB are separated by 36 nucleotides
abgB and ydaJ overlap by 1 nucleotides
ydaJ and abgR are separated by 175 nucleotides
abgR and isrA are separated by 2 nucleotides
b1336: ydaH - putative pump protein (transport) (VIMSS), at 1,398,271 to 1,399,797
ydaH
b1337: abgB - predicted peptidase, aminobenzoyl-glutamate utilization protein (NCBI), at 1,399,834 to 1,401,279
abgB
b1338: ydaJ - putative aminohydrolase (EC 3.5.1.14) (VIMSS), at 1,401,279 to 1,402,589
ydaJ
b1339: abgR - predicted DNA-binding transcriptional regulator (NCBI), at 1,402,765 to 1,403,673
abgR
b4426: isrA - unknown RNA (NCBI), at 1,403,676 to 1,403,833
isrA
Group
Condition
b1336
b1337
b1338
b1339
b4426
ydaH
abgB
ydaJ
abgR
isrA
predation
6 hr rep1_HD100_10fold_dilution_grewON
+0.8
-2.6
+0.0
+0.3
-6.7
carbon source
carbon source D-Glucose 0.25mM
+0.5
-2.9
-1.2
+0.1
+0.2
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
-1.3
-0.5
-0.9
-0.8
+0.8
carbon source
carbon source D-Mannose 5mM
+0.2
+0.2
-0.5
+0.7
-3.2
stress
Trimethoprim 0.005 mM
-0.3
-1.9
+0.4
-0.3
-0.4
phage
N4_phage 0.009375 MOI TC2
+0.2
-1.9
-0.4
-1.4
+1.0
stress
LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
+0.1
+0.5
-0.7
+0.2
-2.6
attachment_assay_attached_sample
Time0_Wash=PBS; Attachment_time=2hrs; Attachment_surface=PDMS; Sample_type=attached; Outgrowth=LB
-0.4
+0.6
-1.0
-0.4
-1.1
stress
Chloramphenicol 0.005 mg/ml
+0.4
+0.6
-0.7
-0.2
-1.8
carbon source
carbon source D-Mannose 0.5mM
+0.6
-1.5
-0.2
-0.4
+0.4
carbon source
carbon source D-Glucose 0.25mM
+0.7
+0.6
-0.0
+0.2
-2.6
phage
T7_phage 0.00015625 MOI TC4
-1.1
+0.2
-0.9
+0.8
+0.1
motility
outer cut, LB soft agar motility assay
+0.3
+1.7
+0.0
-0.3
-2.1
phage
Δdbn_10
-0.0
-1.8
+0.4
+0.7
+0.7
phage
Ste41
+0.0
+0.1
-1.7
+0.1
+1.6
stress
LB Anaerobic with Cefoxitin 0.000003125 mM
-0.5
-0.4
-0.3
-0.3
+1.7
plasmid transformation
vcDART vector transformation; liquid outgrowth assay
+0.6
-0.4
-0.5
-0.5
+1.3
phage
T7_phage 0.0015625 MOI TC2
-0.8
+1.6
-0.2
+0.6
-0.5
stress
M9 with Methotrexate 1.98 mM 24 hr
+0.1
-0.2
-1.0
-0.1
+1.8
plasmid transformation
mariner vector transformation; liquid outgrowth assay
+1.1
-0.2
-1.0
+0.2
+1.0
nitrogen source
Gly-Glu (N)
-0.2
+0.2
+1.9
-0.2
-0.5
stress
Cisplatin 0.1 mg/ml
-0.3
+1.2
-0.1
-0.4
+1.0
carbon source
carbon source D-Glucose 5mM
+0.9
+0.8
+0.1
+0.9
-1.1
control
PBS_cage_1_mouse_2_Cecum_40 hr
+0.8
-0.7
-0.4
+0.3
+1.9
stress
LB Anaerobic with Ciprofloxacin Hydrochloride 0.0000000390625 mM
-0.5
+0.7
-0.2
+0.8
+1.5
nitrogen source
Gly-Glu (N)
+0.2
+0.3
+2.0
+0.3
-0.5
stress
LB Anaerobic with Benzethonium chloride 0.00003125 mM
+0.1
+0.9
+0.3
+0.7
+1.1
control
PBS_cage_1_mice _Feces_16 hr
+0.5
+0.8
+0.2
+0.2
+1.4
control
PBS_cage_1_mice _Feces_22 hr
+0.5
+1.0
+0.3
+0.2
+1.1
carbon source
carbon source D-Glucose 20mM
+0.5
+0.6
+0.3
+0.2
+1.6
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